Results 41 to 50 of about 734,685 (383)
Real-time polymerase chain reaction in acute retinal necrosis following encephalitis
Indian Journal of Ophthalmology, 2018 Acute retinal necrosis (ARN) following herpes simplex encephalitis (HSE) in an immunocompetent patient is a rare condition. Quantitative real-time polymerase chain reaction (qPCR) has made it possible to identify and quantify viral genome.
Vinita Rao +2 more
doaj +1 more source
Memórias do Instituto Oswaldo Cruz, 2017 We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT ...
F. Naveca +5 more
semanticscholar +1 more source
Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma [PDF]
, 2015 Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy.
Asadi, J. +9 more
core +1 more source
A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test [PDF]
, 2014 A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species.
De Craeye, Stéphane +6 more
core +4 more sources
Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains [PDF]
Archives of Razi Institute, 2019 Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents.
S. Alamian +4 more
doaj +1 more source
Quantification Strategies in Real-time Polymerase Chain Reaction
Polymerase Chain Reaction: Theory and Technology, 2019 The present chapter describes the quantification strategies used in real-time RT-PCR (RT-qPCR), focusing on the main elements that are essential to fulfil the MIQE guidelines.
M. Pfaffl
semanticscholar +1 more source
Detection of medically important Candida species by absolute quantitation real-time polymerase chain reaction [PDF]
, 2014 Background: Chronic obstructive pulmonary disease (COPD) is one of the most important causes of disability and mortality in the world. Although cigarette smoking and environmental pollutants have been recognized as the major causes of COPD, the role of ...
Chong, Pei Pei +3 more
core +2 more sources
Revista Brasileira de Hematologia e Hemoterapia, 2015 Introduction: Minimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia.
Francisco Danilo Ferreira Paula +7 more
doaj +1 more source
Real-time reverse transcription polymerase chain reaction [PDF]
, 2011 RT-qPCR is one of the most widespread and reliable methods of measuring gene expression. First, the extraction of mRNA from a homogeneous cell group is done in order to perform the RT phase (reverse transcription). At this point the reverse transcriptase
Ferreira, Marcus Castro +2 more
core +2 more sources
International Journal of Advanced Medical and Health Research, 2021 Background: The influenza pandemics have caused serious morbidity and mortality around the world. Only a few studies have described the clinical profile of both influenza A and B infections among children and its seasonal variations.
Janani Arul +4 more
doaj +1 more source