Results 41 to 50 of about 644,089 (298)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

Reference gene selection for quantitative real-time polymerase chain reaction analysis [PDF]

open access: yesInvertebrate Survival Journal, 2017
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most serious viral disease in silkworms. To investigate the mechanisms of the immune responses of B.
Z Nie   +7 more
doaj  

Clinical profile of children with influenza a and b infections admitted to a tertiary care hospital in South India

open access: yesInternational Journal of Advanced Medical and Health Research, 2021
Background: The influenza pandemics have caused serious morbidity and mortality around the world. Only a few studies have described the clinical profile of both influenza A and B infections among children and its seasonal variations.
Janani Arul   +4 more
doaj   +1 more source

Problem‐solving test: Real‐time polymerase chain reaction [PDF]

open access: yesBiochemistry and Molecular Biology Education, 2009
AbstractTerms to be familiar with before you start to solve the test: polymerase chain reaction, DNA amplification, electrophoresis, breast cancer, HER2 gene, genomic DNA, in vitro DNA synthesis, template, primer, Taq polymerase, 5′→3′ elongation activity, 5′→3′ exonuclease activity, deoxyribonucleoside triphosphates, DNA structure, proofreading ...
openaire   +2 more sources

Detection of medically important Candida species by absolute quantitation real-time polymerase chain reaction [PDF]

open access: yes, 2014
Background: Chronic obstructive pulmonary disease (COPD) is one of the most important causes of disability and mortality in the world. Although cigarette smoking and environmental pollutants have been recognized as the major causes of COPD, the role of ...
Chong, Pei Pei   +3 more
core   +2 more sources

Characterizing the salivary RNA landscape to identify potential diagnostic, prognostic, and follow‐up biomarkers for breast cancer

open access: yesMolecular Oncology, EarlyView.
This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan   +9 more
wiley   +1 more source

Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]

open access: yes, 2009
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E.   +3 more
core   +2 more sources

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

open access: yesMolecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts   +8 more
wiley   +1 more source

Multiplex Real-Time Polymerase Chain Reaction on Sputum for the Diagnosis of Pneumocystis jirovecii Pneumonia in Children: A Retrospective Study

open access: yesInfection and Drug Resistance, 2021
Juan Jiang,1,2 Xia Wang,3 Jian He,3 Donglei Liao,3 Xiaolu Deng3 1Department of Respiratory Medicine (National Key Clinical Specialty), Xiangya Hospital, Central South University, Changsha, People’s Republic of China; 2Hunan Provincial Clinical Research ...
Jiang J, Wang X, He J, Liao D, Deng X
doaj  

Identification of Exiguobacterium acetylicum by a double real- time polymerase chain reaction assay

open access: yes浙江大学学报. 农业与生命科学版, 2017
Exiguobacterium sp. has been figured out to be the main spoilage bacteria on many kinds of food, and it has the potential to be the main spoiler. Bacteria Exiguobacterium have already been isolated from extreme environment and they have evolved and ...
GAO Cancan   +7 more
doaj   +1 more source

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