Results 91 to 100 of about 993,329 (331)

A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water [PDF]

, 2015
Background: Water and High Purity Water (HPW) distribution systems can be contaminated with human pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in the industrial, pharmaceutical and clinical ...
Barry, Thomas, Minogue, Elizabeth, Reddington, Kate, Smith, Cindy J., Tuite, Nina L   +4 more
core   +3 more sources

Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines

Molecular Oncology, EarlyView.
Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad, Mahmood Hachim, Tom Bertin, Karolina Penderecka, Rifat Hamoudi, Saif Khan, Rui Henrique, Natalie Bergmoser, Manit Arya, Kalle Sipila, Matteo Vietri Rudan, Asif Muneer, Aamir Ahmed   +12 more
wiley   +1 more source

PCR-based assays for detecting Xanthomonas axonopodis pv. allii in onion seed [PDF]

, 2012
Bacterial blight of onion is an emerging disease threatening world onion production, and causing damage to other Allium crops. The causal agent, Xanthomonas axonopodis pv.
Chabirand, Aude, Jouen, Emmanuel, Perret, Marion, Pruvost, Olivier, Robène-Soustrade, Isabelle   +4 more
core  

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

自发性高血压大鼠血液miRNAs表达分析

Zhongguo shiyan zhenduanxue, 2014
目的本试验检测自发性高血压大鼠（SHR）血液中miR-448-5p、miR-128、miR-146a和miR-24-2*四种小分子RNA（miRNA）的表达,探讨高血压疾病与miRNA表达水平相关性,为人类高血压诊断及治疗提供新的靶标。方法应用荧光定量PCR方法对SHR大鼠和正常大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2*表达量进行检测。结果与正常对照组相比,SHR大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2 ...
鞠传静, 王旭渤, 梁斌斌, 李颖, 于冰   +4 more
doaj  

The presence of extracellular microRNAs in the media of cultured Drosophila cells [PDF]

, 2018
While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking.
Gansemans, Yannick, Gijbels, Marijke, Mingels, Lina, Santos, Dulce, Van den Brande, Stijn, Van Nieuwerburgh, Filip, Vanden Broeck, Jozef, Wynant, Niels   +7 more
core   +3 more sources

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction [PDF]

, 2005
Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.
Chen, Y, He, ML, Kung, HF, Li, Z, Lin, MC, Lu, GX, Peng, Y, Qian, WP, Shing, LK, Tan, YQ   +9 more
core   +1 more source

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source

Quantitative real time PCR for distinction between Pneumocystis jirovecii infection/colonization in hospitalized patients

Frontiers in Cellular and Infection Microbiology
BackgroundIdentification of the opportunistic fungus Pneumocystis jirovecii in respiratory specimens presents challenges, particularly in differentiating between colonization and active infection.
Faezeh Rouhi, Mahzad Erami, Sepide Rastgufar, Maryam Jahani, Shima Aboutalebian, Shima Aboutalebian, Sajedeh Soltani, Hamed Fakhim, Hossein Mirhendi, Hossein Mirhendi   +9 more
doaj   +1 more source