Brazilian Journal of Medical and Biological Research, 2009 Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR)
A.C.M.F. Souza +4 more
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The prognostic impact of EGFR, ErbB2 and MET gene amplification in human gastric carcinomas as measured by quantitative Real-Time PCR [PDF]
, 2015 Purpose: Identification of critical genes which play pivotal roles in controlling tumor growth and survival will establish the basis for developing therapeutic targets. In this study, we focused on frequencies of EGFR, ErbB2 and MET gene amplification in
Farazmandfar, T. +4 more
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Frontiers in Cellular and Infection Microbiology, 2022 Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang +7 more
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Investigations into the utility of real-time PCR for the detection, quantitation and characterisation of clinically relevant viruses [PDF]
, 2003 The use of PCR as a tool for the diagnostic virology and viral research laboratories has greatly increased in recent years, however the use of conventional PCR and amplicon detection systems can be a complex and relatively slow process that increases the
Mackay, Ian M.
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Evaluation and comparison of SYBR Green I Real-Time PCR and TaqMan Real-Time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk [PDF]
, 2012 Specific traditional plate count method and real-time PCR systems based on SYBR Green I and TaqMan technologies using a specific primer pair and probe for amplification of iap-gene were used for quantitative assay of Listeria monocytogenes in seven ...
Bassami, M R +6 more
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Differential cytokine responses following Marek's disease virus infection in chicken genotypes differing in resistance to Marek's disease [PDF]
, 2003 The production of cytokine mRNAs, in addition to viral DNA, was quantified by real-time quantitative reverse transcription-PCR (RT-PCR) (cytokines) or PCR (virus) in splenocytes during the course of Marek's disease virus (MDV) infection in four inbred ...
Davison, T F, Kaiser, Pete, Underwood, G
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Development of a droplet digital PCR method for detection of porcine circovirus 4
BMC Veterinary Research, 2023 Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu +4 more
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Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR
BioTechniques, 2022 For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian +6 more
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Physical Simulation-Based Calibration for Quantitative Real-Time PCR
Applied SciencesThe fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
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Quantitative real-time PCR data analysis with R [PDF]
, 2016 This dissertation covers an introduction to the field of qPCR data analysis using the state-of-the-art R programming language. qPCR analyses genomic data based on the DNA replication.
Machado de Souza, Ignacio Nicolás
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