Results 51 to 60 of about 1,064,076 (204)
Quantitative real-time PCR for determination of Transgene in Callus of Jatropha curcas [PDF]
, 2014 Jatropha curcas is an important plant belonging to the family Euphorbiaceae which is a potential candidate for biofuel production. Genetic transformation protocol for J.
Jualang Azlan Gansau +3 more
core +1 more source
Journal of Veterinary Research, 2022 Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease.
Souza Camilla de +6 more
doaj +1 more source
Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes [PDF]
, 2008 Background Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was
Mihaela Skulj +6 more
core +2 more sources
BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
BMC Microbiology, 2012 Background Bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. Based on our analysis of a diverse collection of 16 S rRNA
Liu Cindy M +6 more
doaj +1 more source
Serum CCAT2 as a biomarker for adjuvant diagnosis and prognostic prediction of cervical cancer
Journal of Ovarian Research, 2022 Growing evidence indicates that lncRNA colon cancer-associated transcript 2 (CCAT2) is associated with cancers. However, the clinical value of CCAT2 in cervical cancer (CC) remains unclear.
Xiaoli Cao +5 more
doaj +1 more source
Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species [PDF]
, 2016 This study was supported by the National Natural Science Foundation of China, 31471823, the Earmarked Fund for China Agriculture Research System, CARS-35-07, and the Innovation Project of Chinese Academy of Agricultural Science.Studies on grasshopper ...
Cao, Guangchun +9 more
core +2 more sources
Rapid titer determination using quantitative real-time PCR [PDF]
Gene Therapy, 1999 Quantitative real-time PCR was utilized to evaluate retroviral vector titer. RNA was prepared from vector supernatant and run in a one-step RT-PCR reaction combining reverse transcription (RT) and amplification in one tube. Sample analysis was performed in the ABI Prism 7700 Sequence Detector.
N, Sanburn, K, Cornetta
openaire +2 more sources
BMC Genomics, 2022 Background Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s).
Jeongah Song +3 more
doaj +1 more source
Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]
, 2019 Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An +2 more
core +1 more source