Results 121 to 130 of about 574,812 (244)

Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening

Advanced Healthcare Materials, EarlyView.
Models of the human skin must combine the relevant biological contents and suitable biomaterials with the correct spatial organization. Performing compound screening on such in vitro models also requires fast and reproducible production methods of the models.
Elisa Lenzi, Nadia Vertti‐Quintero, Julien Husson, Anne‐Laure Bulteau, Carine Nizard, Karl Pays, Sébastien Sart, Charles N. Baroud   +7 more
wiley   +1 more source

Comparison of Three RT-PCR Based Methods for Relative Quantification of mRNA

Food Technology and Biotechnology, 2005
Comparison of three RT-PCR based methods: semi-quantitative, competitive and real--time RT-PCR for relative quantification of mRNA is presented. Aminopeptidase N expressed on human promyeloid HL-60 cell line, at basal and activated state, served as a ...
Andreja Ambriović-Ristov, Sanja Kapitanović, Tamara Čačev, Jelka Gabrilovac, Davorka Breljak   +4 more
doaj  

Preliminary Study of a Ninj1‐Loaded Bimodal Ultrasound/NIR Fluorescence Targeted Molecular Probe for Diagnosing Early‐Stage Inflammation in Coronary Microvascular Dysfunction

Advanced Healthcare Materials, EarlyView.
Coronary microvascular dysfunction (CMD) targeting remains a challenge for precise diagnosis. This work presents a dual‐modal nanoprobe (T‐IR780‐NBs) that combines ultrasound contrast with near‐infrared fluorescence. This technology utilizes proteomics‐derived antibodies that specifically localize to inflamed and injured cardiac tissue, enabling ...
Xiaohui Xu, Lina Guan, Baihetiya Tayier, Chen Yuan, Shangke Chen, Qi Shi, Yuming Mu   +6 more
wiley   +1 more source

Quantitative real-time RT-PCR based transcriptomics:

, 2006
Die quantitative Reverse-Transkription mit Polymerase-Kettenreaktion (qRT-PCR) ist eine neue Methode, um geringste mRNA Mengen in biologischen Proben zuverlässig zu bestimmen. Da die Stärke der Fluoreszenz des Reporterfarbstoffes proportional zur gebildeten DNA-Menge sein sollte, ermöglicht die Aufnahme der Fluoreszenz eine Darstellung des gesamten ...
openaire   +1 more source

A 3D Bioprinted Spheroid‐Laden dECM‐Enriched Osteosarcoma Model for Enhanced Drug Testing and Therapeutic Discovery

Advanced Healthcare Materials, EarlyView.
A 3D biomimetic OS model was developed by bioprinting an OS‐cell‐derived dECM‐enriched bioink with OS spheroids incorporated. The model showed upregulation of known OS prognostic markers and increased resistance to doxorubicin, compared to 2D cultures and scaffold‐free spheroids, making this a more clinically relevant platform for drug discovery ...
Margarida F. Domingues, Maria Catarina Carreira, Mafalda S. Santos, Daniela Pacheco, Frederico Castelo Ferreira, Paola Sanjuan‐Alberte, João Carlos Silva   +6 more
wiley   +1 more source

Parechovirus real-time RT-PCR v2 [PDF]

, 2017
openaire   +1 more source

SiO2‐CaOCME/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin   +3 more
wiley   +1 more source

Sensitive detection and differentiation of rhinoviruses and enteroviruses by a nested real-time RT-PCR assay. [PDF]

Microbiol Spectr
Ogura M, Ohnishi T, Yaginuma M, Kobayashi H, Furuichi M, Inose R, Kato S, Shinjoh M.   +7 more
europepmc   +1 more source

Development, Evaluation, and Clinical Application of PRRSV-2 Vaccine-like Real-Time RT-PCR Assays. [PDF]

Viruses, 2023
Rawal G, Krueger KM, Yim-Im W, Li G, Gauger PC, Almeida MN, Aljets EK, Zhang J.   +7 more
europepmc   +1 more source

Targeting the ARRDC3–DRP1 Axis via hUMSC‐Derived Exosomal CRYAB for Neuroprotection in Cerebral Ischemia/Reperfusion Injury

Advanced Healthcare Materials, EarlyView.
Intranasally administered hUMSC‐derived exosomes modulate the CRYAB–ARRDC3–Drp1 axis, alleviating mitochondrial dysfunction and ferroptosis, enhancing neuronal survival, reducing oxidative stress, and promoting functional recovery in ischemia‐reperfusion injury, offering a promising therapeutic strategy for ischemic stroke.
Rong ji, Zengyu Zhang, Zhuohang Liu, Kaicheng Yang, Xueyu Mao, Min Chu, Yong Wang, Jing Zhao   +7 more
wiley   +1 more source