Results 121 to 130 of about 814,412 (288)
Infectio, 2008 Objetivo. Detectar Salmonella spp. por PCR en tiempo real (PCR-TR) y el método convencional en alimentos de la vía pública y canales de bovino de una planta certificada de beneficio animal con sistema HACCP de Montería. Materiales y métodos.
Edna Yánez, Salim Máttar, Alba Durango
doaj
Quantification of infectious recombinant murine MSCV-based retroviruses using PCR approach [PDF]
, 2010 The current available molecular method to detect the retroviral-mediated gene expression is reverse transcriptase-polymerase chain reaction (RT-PCR). However, the conventional PCR is time consuming, prone to error and not sensitive.
Allaudin, Zeenathul Nazariah +4 more
core
DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling
FEBS Open Bio, EarlyView.Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang +7 more
wiley +1 more source
Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
Ciência Rural: The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis.
Thor Vinícius Martins Fajardo +2 more
doaj +1 more source
Comparison Of Extraction Methods For Detecting Hepatitis A Virus In Shellfish (Mystilus Galloprovincialis) Using Tissue Culture Titration, RT-Nested PCR And Real Time RT-PCR [PDF]
, 2009 Thirty commercial shellfish samples (22 cockles and 8 surf cockles) were collected from several locations around Serdang and tested for the presence of hepatitis A virus (HAV) using a modification of the glycine, polyethylene glycol, Tri-reagent, poly dT
Yong, Han Tek
core
Japanese encephalitis virus real-time RT-PCR v3
, 2018 A real-time RT-PCR using an MGB probe, this assay detects Japanese encephalitis virus (JEV) from human and mosquito samples. The assay targets the 3'UTR region of known JEV strains.
Judy A Northill +3 more
openaire +1 more source
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
FEBS Open Bio, EarlyView.Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Virology Journal, 2010 A duplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay was improved for simultaneous detection of highly pathogenic H5N1 avian influenza virus and pandemic H1N1 (2009) influenza virus, which is suitable for early diagnosis of ...
Qin E-de +9 more
doaj +1 more source
Aichivirus real-time RT-PCR 2007 method v1 [PDF]
, 2019 A real-time assay to detect Aichivirus A (AiV-A) in human samples. It is based in the 3'UTR region and has been employed in our laboratory since 2007.
openaire +1 more source
FEBS Open Bio, EarlyView.Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava +3 more
wiley +1 more source