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Biotransformation of stevioside to rebaudioside – A in suspension cells of Stevia: Applications of leaf extract in functional biscuits

Research journal of biotechnology, 2022
The suspension culture of Stevia was conceived as a means of establishing a stevioside-producing cell line in vitro. The highest callus induction rate (90%) was achieved using the Stevia leaf explants in modified MS medium supplemented with 1 mg/L BAP ...
R. Shahu, P. Nambiar, R. Jobby, P. Jha
semanticscholar   +1 more source

Genotoxicity studies on a high-purity rebaudioside A preparation

Food and Chemical Toxicology, 2009
Rebaudioside A (Reb A) is a steviol glycoside isolated from the leaves of the Stevia rebaudiana plant. This non-nutritive, natural sweetener is reported to be 250-450 times sweeter than sucrose and has potential for wide use in the US diet, and is used in Japan and South America today.
Lonnie D, Williams, George A, Burdock
exaly   +3 more sources

NMR studies of the conformation of the natural sweetener rebaudioside A

Carbohydrate Research, 2009
Rebaudioside A is a natural sweetener from Stevia rebaudiana in which four beta-D-glucopyranose units are attached to the aglycone steviol. Its (1)H and (13)C NMR spectra in pyridine-d(5) were assigned using 1D and 2D methods. Constrained molecular dynamics of solvated rebaudioside using NMR constraints derived from ROESY cross peaks yielded the ...
Wayne E, Steinmetz, Alvin, Lin
openaire   +2 more sources

Developing polyploid genetic resources for enhanced rebaudioside A synthesis and agronomic traits in Stevia rebaudiana Bertoni

Plant genetic resources
Stevia rebaudiana Bertoni stores over 20 glycosides in leaves and stem, the primary sweetening compounds are stevioside and rebaudioside A. Stevioside is 250–300 times and rebaudioside A is 350–450 times sweeter than sucrose.
Sivendra Joshi   +4 more
semanticscholar   +1 more source

Phytocompounds hesperidin, rebaudioside a and rutin as drug leads for the treatment of tuberculosis targeting mycobacterial phosphoribosyl pyrophosphate synthetase

Journal of Biomolecular Structure and Dynamics
The main aim of this study is to address the global health crisis posed by tuberculosis (TB) through the exploration of novel therapeutic strategies targeting Mycobacterial phosphoribosyl pyrophosphate synthetase (MtPrsA), an untried enzyme involved in ...
Sneha Manthattil Vysyan   +4 more
semanticscholar   +1 more source

The non-cytotoxicity characterization of rebaudioside A as a food additive

Food and Chemical Toxicology, 2014
To evaluate the cytotoxicity of high-purity rebaudioside A (reb A, 99.16%) as a food ingredient, a combination of several methods, including tetrazolium-based colorimetric assay (MTT), lactate dehydrogenase assay (LDH), enzyme-linked immunosorbent assay (ELISA), real-time PCR (qPCR), high-performance liquid chromatography (HPLC), and two-dimensional ...
Xiaoli, Wu   +7 more
openaire   +2 more sources

Rebaudioside, a novel agent of glycoside clathration of pharmacons

Doklady Biological Sciences, 2009
205 Glycyrrhizinic acid and stevioside, glycosides of higher terpenoids, are capable of in vivo formation of clathrates with pharmacons with various types of activity. This property of glycosides allows the effective dose to be substantially decreased (by a factor of 10– 100), with the basic activity being preserved and novel pharmacological effects ...
T G, Tolstikova   +3 more
openaire   +2 more sources

Investigations on the Stability of Stevioside and Rebaudioside A in Soft Drinks

Journal of Agricultural and Food Chemistry, 2010
The stability of the two steviol glycosides stevioside and rebaudioside A and the possible formation of the aglycon steviol in different soft drinks were analyzed in samples spiked with stevioside or rebaudioside A after 24, 48, and 72 h storage times at 80 °C.
Ursula, Wölwer-Rieck   +2 more
exaly   +3 more sources

Combined enzymatic modification of stevioside and rebaudioside A

Applied Biochemistry and Microbiology, 2006
Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by B. stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. This method can be
V. T. Kochikyan   +4 more
openaire   +3 more sources

Production of rubusoside from high concentration of stevioside with or without rebaudioside A and its performance in micelle solubilization

, 2021
Rubusoside is a natural botanic sweetener with capacity of micelle solubilization. This study revealed the reaction pathway of enzymatically producing rubusoside from a high concentration of stevioside in the absence or presence of rebaudioside A, the ...
Zhuoyu Zhou   +6 more
semanticscholar   +1 more source

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