Results 41 to 50 of about 166,825 (267)

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

BRCA1 protein expression and subcellular localization in primary breast cancer: Automated digital microscopy analysis of tissue microarrays.

PLoS ONE, 2017
PurposeMutations in BRCA1 are associated with familial as well as sporadic aggressive subtypes of breast cancer, but less is known about whether BRCA1 expression or subcellular localization contributes to progression in population-based settings ...
Abeer M Mahmoud, Virgilia Macias, Umaima Al-Alem, Ryan J Deaton, Andre Kadjaksy-Balla, Peter H Gann, Garth H Rauscher   +6 more
doaj   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

Structure-function analysis of barley NLR immune receptor MLA10 reveals its cell compartment specific activity in cell death and disease resistance. [PDF]

PLoS Pathogens, 2012
Plant intracellular immune receptors comprise a large number of multi-domain proteins resembling animal NOD-like receptors (NLRs). Plant NLRs typically recognize isolate-specific pathogen-derived effectors, encoded by avirulence (AVR) genes, and trigger ...
Shiwei Bai, Jie Liu, Cheng Chang, Ling Zhang, Takaki Maekawa, Qiuyun Wang, Wenkai Xiao, Yule Liu, Jijie Chai, Frank L W Takken, Paul Schulze-Lefert, Qian-Hua Shen   +11 more
doaj   +1 more source

Familial ALS-associated SFPQ variants promote the formation of SFPQ cytoplasmic aggregates in primary neurons

Open Biology, 2022
Splicing factor proline- and glutamine-rich (SFPQ) is a nuclear RNA-binding protein that is involved in a wide range of physiological processes including neuronal development and homeostasis.
Jocelyn Widagdo, Saumya Udagedara, Nishita Bhembre, Jing Zhi Anson Tan, Lara Neureiter, Jie Huang, Victor Anggono, Mihwa Lee   +7 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Deep learning for automated scoring of immunohistochemically stained tumour tissue sections – Validation across tumour types based on patient outcomes

Heliyon
We aimed to develop deep learning (DL) models to detect protein expression in immunohistochemically (IHC) stained tissue-sections, and to compare their accuracy and performance with manually scored clinically relevant proteins in common cancer types.Five
Wanja Kildal, Karolina Cyll, Joakim Kalsnes, Rakibul Islam, Frida M. Julbø, Manohar Pradhan, Elin Ersvær, Neil Shepherd, Ljiljana Vlatkovic, Xavier Tekpli, Øystein Garred, Gunnar B. Kristensen, Hanne A. Askautrud, Tarjei S. Hveem, Håvard E. Danielsen, Tone F. Bathen, Elin Borgen, Anne-Lise Børresen-Dale, Olav Engebråten, Britt Fritzman, Olaf Johan Hartman-Johnsen, Øystein Garred, Jürgen Geisler, Gry Aarum Geitvik, Solveig Hofvind, Rolf Kåresen, Anita Langerød, Ole Christian Lingjærde, Gunhild M. Mælandsmo, Bjørn Naume, Hege G. Russnes, Kristine Kleivi Sahlberg, Torill Sauer, Helle Kristine Skjerven, Ellen Schlichting, Therese Sørlie   +35 more
doaj   +1 more source

Estrogen Signaling in ERα-Negative Breast Cancer: ERβ and GPER

Frontiers in Endocrinology, 2019
Estrogen receptors are important regulators of the growth of breast tumors. Three different receptors for estrogens have been identified in breast tumors, two nuclear receptors, ERα and ERβ, and a G-protein coupled estrogen receptor 1 (GPER) that ...
Rainer Girgert, Günter Emons, Carsten Gründker   +2 more
doaj   +1 more source

Quantitative Estimates of Cytoplasmic and Nuclear Oestrogen Receptors in Chick Oviduct [PDF]

European Journal of Biochemistry, 1976
[3H]Oestradiol exchange techniques were developed for the determination of specific cestrogen receptor site concentrations in the cytoplasm and nuclei of chick oviduct cells. Non‐labelled receptor‐bound oestrogens were exchanged with [3H]oestradiol during a 24‐h incubation at 20 °C. 2 h at 30 °C or 45 min at 37 °C.
R L, Sutherland, E E, Baulieu
openaire   +2 more sources