Results 101 to 110 of about 2,004 (138)

Characterization of a Specific Somatomedin-C Receptor on Isolated Bovine Growth Plate Chondrocytes*

Endocrinology, 1983
The interaction of somatomedin (Sm) with growth plate chondrocytes (GPCs) is believed to be the primary stimulus of skeletal growth. Using techniques designed to disrupt as little as possible the phenotypic characteristics of GPCs, we have been able to obtain 3-4 x 10(8) viable cells from the major physes of one newborn calf.
S B, Trippel, J J, Van Wyk, M B, Foster, M E, Svoboda   +3 more
openaire   +2 more sources

Somatomedin-C Receptor Ontogeny and Levels in Porcine Fetal and Human Cord Serum

The Journal of Clinical Endocrinology & Metabolism, 1976
The ontogeny of somatomedin receptors in tissues of fetal pigs and levels of somatomedin-C in fetal pig serum at various gestational ages and in human cord serum was investigated. Specific binding of 125I somatomedin-C by particulate membranes prepared from fetal organs from a variety of gestational ages almost always exceeds specific 125I insulin ...
A J, D'ercole, D B, Foushee, L E, Underwood   +2 more
openaire   +2 more sources

A RECEPTOR ASSAY FOR SOMATOMEDIN‐LIKE ACTIVITY USING MULTIPLICATION‐STIMULATING ACTIVITY (MSA) AS RADIOLIGAND

Australian Journal of Experimental Biology and Medical Science, 1978
SummaryMultiplication‐stimulating activity (MSA) in the medium of cultured rat liver cells was purified 120‐fold by chromatography on Dowex 50W and Sephadex G 50. MSA was labelled with 125I‐iodine and bound to a human placental membrane receptor. An assay for somatomedin‐like receptor activity (SmLRA) in unextracted serum was based on the displacement ...
M W, Brinsmead, G C, Liggins
openaire   +2 more sources

Demonstration of type I and type II somatomedin receptors on bovine growth plate chondrocytes

Journal of Orthopaedic Research, 1988
AbstractThe chondrocytes of the epiphyseal growth plate are the presumed target cells for hormones regulating skeletal growth. The somatomedins, a family of low molecular weight peptides, are thought to play a stimulatory role in this regulation. The cellular actions of the somatomedins are themselves determined by binding to specific receptors on ...
S B, Trippel, S D, Chernausek, J J, Van Wyk, A C, Moses, H J, Mankin   +4 more
openaire   +2 more sources

Structural similarities between human receptors for somatomedin C and insulin: analysis by affinity labeling

Biochemistry, 1981
Human placental receptors for insulin and somatomedin C (Sm-C) were affinity labeled with [125I]insulin and [125I]Sm-C by using the bifunctional cross-linking agent disuccinimidyl suberate. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated that both labeled hormones were specifically cross-linked to three protein ...
S D, Chernausek, S, Jacobs, J J, Van Wyk
openaire   +2 more sources

Insulin and Insulin-Like Growth Factor (Somatomedin) Receptors on Cloned Rat Pituitary Tumor Cells*

Endocrinology, 1985
Specific receptors for insulin and the somatomedin peptides insulin-like growth factors I and II (IGF-I and IGF-II) have been characterized on three separate cloned strains of rat pituitary tumor cells (GH3, GH1, and GC). Binding of 125I-labeled peptides was time, temperature, and pH dependent for all three cell lines. Specific binding of [125I]insulin,
ROSENFELD RG, CUTLER CW, DOLLAR LA, HOFFMAN AR, CEDA, Gian Paolo   +4 more
openaire   +2 more sources

Characterization of a specific insulin-like growth factor-I/somatomedin-C receptor on high density, primary monolayer cultures of bovine articular chondrocytes: regulation of receptor concentration by somatomedin, insulin and growth hormone

Journal of Endocrinology, 1985
ABSTRACT In order to obtain a phenotypically stable cell population of chondrocytes, high density primary monolayer cultures of bovine articular chondrocytes were established. Using these cultures, a specific insulin-like growth factor-I/somatomedin-C (IGF-I/SM-C) receptor was demonstrated and characterized.
N, Watanabe, R G, Rosenfeld, R L, Hintz, L A, Dollar, R L, Smith   +4 more
openaire   +2 more sources

Explorations of the Insulinlike and Growth-Promoting Properties of Somatomedin by Membrane Receptor Assays

1975
Publisher Summary This chapter presents the explorations of the insulinlike and growth-promoting properties of somatomedin by membrane receptor assays. A competitive radioreceptor assay using 125 I-labeled insulin and a human placental cell membrane preparation was developed to measure the total insulinlike activity (ILA) during the fractionation of
J J, van Wyk, L E, Underwood, J B, Baseman, R L, Hintz, D R, Clemmons, R N, Marshall   +5 more
openaire   +2 more sources

Somatomedin Receptors

1987
Paul B. Kaplowitz, Steven D. Chernausek
openaire   +1 more source

Somatomedin Receptors: Structure, Function, and Regulation

1986
RON G. ROSENFELD, RAYMOND L. HINTZ
openaire   +1 more source