Results 161 to 170 of about 15,672 (212)

Assays for Vasoactive Intestinal Peptide Receptor

1991
Publisher Summary This chapter discusses the methods for characterization of the number and affinity of vasoactive intestinal peptide (VIP) receptor sites on intact cells, in membrane preparations, and in solubilized membranes. The chapter also describes experiments for determining the pharmacologic specificity of peptide receptors, and for using ...
Gail D. Wenger, M. Sue O'Dorisio, Laura B. Campolito   +2 more
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Receptors for vasoactive intestinal peptide and secretin on small intestinal epithelial cells

American Journal of Physiology-Gastrointestinal and Liver Physiology, 1980
Binding of 125I-labeled vasoactive intestinal peptide (VIP) to dispersed enterocytes prepared from guinea pig small intestine was saturable, temperature dependent, and reversible, and reflected interaction of the labeled peptide with a single class of binding sites. Each enterocyte possessed approximately 60,000 binding sites and binding of the tracer
H J, Binder, G F, Lemp, J D, Gardner
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Vasoactive intestinal peptide receptor expression in chronic periapical lesions

International Endodontic Journal, 2007
AbstractAim  To use radioreceptor analysis for evaluating whether vasoactive intestinal peptide (VIP) receptors are present in chronic periapical lesions and to determine whether differences in its expression are found according to the size of the lesions.Methodology  Twelve periapical lesions were obtained from teeth diagnosed with chronic apical ...
J, Caviedes-Bucheli, M M, Azuero-Holguín, G C, Moreno, I L, González, E, Mateu, J F, Salazar, H R, Munoz   +6 more
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Vasoactive intestinal peptide (VIP) receptors in the canine gastrointestinal tract

Peptides, 1988
Vasoactive intestinal peptide (VIP) is a putative neurotransmitter in both the brain and peripheral tissues. To define possible target tissues of VIP we have used quantitative receptor autoradiography to localize and quantify the distribution of 125I-VIP receptor binding sites in the canine gastrointestinal tract.
R P, Zimmerman, T S, Gates, C R, Mantyh, S R, Vigna, C G, Boehmer, P W, Mantyh   +5 more
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Expression of vasoactive intestinal peptide (VIP) receptors in human uterus

Peptides, 2000
We show the existence of functional vasoactive intestinal peptide (VIP) receptors in normal human female genital tract (endometrium, myometrium, ovary and Fallopian tube) as well as in leiomyoma (a frequent uterine pathology). The correlation between VIP binding and stimulation of adenylyl cyclase activity for all studied tissues was linear (r = 0.86 ...
A M, Bajo, M G, Juarranz, P, Valenzuela, P, Martínez, J C, Prieto, L G, Guijarro   +5 more
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Characterization and Localization of Vasoactive Intestinal Peptide Receptors in the Rat Lung

Endocrinology, 1984
Experiments were performed to characterize vasoactive intestinal peptide (VIP) receptors in rat lung and to study their localization by means of light and electron microscope autoradiography. [125I]Iodo-VIP binding to lung homogenate was inhibited by synthetic VIP, [Val5]secretin, secretin, and 7-27 secretin with half-maximal inhibition (ID50) values ...
P, Leroux, H, Vaudry, A, Fournier, S, St-Pierre, G, Pelletier   +4 more
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Characterization of vasoactive intestinal peptide receptors in canine liver membranes

Biochemical Pharmacology, 1994
The binding characteristics of vasoactive intestinal peptide (VIP) in the liver membranes of the dog were examined using radioligand binding assay with 125I-VIP and unlabelled peptides and results were compared with those from the rat. The binding of VIP to canine liver membranes occurred in a reversible, saturable, specific and temperature-dependent ...
T, Kiso, S, Ito, T, Ohta, T, Asano, Y, Nakazato   +4 more
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Characterization of vasoactive intestinal peptide receptors on rat alveolar macrophages

American Journal of Physiology-Lung Cellular and Molecular Physiology, 1994
In view of the evidence that vasoactive intestinal peptide (VIP) may modulate acute inflammatory injury in the lung, we investigated the presence and characteristics of VIP receptors on alveolar macrophages (AMs). We examined the binding of monoiodinated [Tyr(125I)10]-labeled VIP (125I-VIP) to rat AMs (> 96% pure), obtained from Sprague-Dawley rats
H, Sakakibara, K, Shima, S I, Said
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Vasoactive intestinal peptide receptor scintigraphy.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 1995
This study presents the biodistribution, safety and absorbed dose of 123I-VIP administered to 18 patients with intestinal adenocarcinomas or endocrine tumors.To achieve high-specific activity, 123I-VIP was purified by HPLC. Following intravenous administration of 123I-VIP (172 +/- 17 MBq (4.65 +/- 0.5 mCi); < 300 pmole ( < 1 microgram)/patient ...
I, Virgolini, A, Kurtaran, M, Raderer, M, Leimer, P, Angelberger, E, Havlik, S, Li, W, Scheithauer, B, Niederle, P, Valent   +9 more
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Role of phospholipids in the binding activity of vasoactive intestinal peptide receptors

Experientia, 1985
Phospholipase digestion of rat intestinal epithelial cell membranes was performed in order to study the influence of membrane phospholipids on the binding activity of VIP receptors. Phospholipases A2 and C strongly (ED50 congruent to 4 X 10(-2) and 4 X 10(-1) micrograms/ml, respectively) and rapidly reduced 125I-VIP binding to membranes whereas ...
A, Sarrieau, N, Boige, M, Laburthe
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