Results 131 to 140 of about 520,105 (221)

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source

Development of a Multiplex Polymerase Chain Reaction Assay for Differentiating Three Lactococcus Species Associated With Piscine Lactococcosis

Journal of Fish Diseases, EarlyView.
ABSTRACT Piscine lactococcosis is an important bacterial disease of farmed fish. The causative agents, Lactococcus garvieae, Lactococcus petauri and Lactococcus formosensis, are closely related, which complicates species‐level identification. We developed a conventional multiplex PCR assay targeting species‐specific genes identified by comparative ...
Tomoaki Yoshino, Tomokazu Takano, Hiroki Tanno, Yukihiro Nakagawa, Yuta Matsuura, Shogo Harakawa, Hidehiro Kondo, Ikuo Hirono, Keiichiro Koiwai, Tomomasa Matsuyama   +9 more
wiley   +1 more source

A Lambda Red Recombinase‐Assisted CRISPR/Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciens

Plant Biotechnology Journal, EarlyView.
ABSTRACT Agrobacterium tumefaciens is a powerful delivery tool for plant genetic transformation and a pivotal platform for agricultural biotechnology. However, laboratory strains of Agrobacterium have remained genetically unoptimized for decades, with genome engineering hindered by inefficient, endogenous homologous recombination systems.
Minmin Zheng, Rui Liu, Wei‐Chang Huang, Zuren Li, Kai Hua   +4 more
wiley   +1 more source

Adenine nucleotide translocator and ATP synthase cooperate in mediating the mitochondrial permeability transition

The Journal of Physiology, EarlyView.
Abstract figure legend Both ATP synthase and adenine nucleotide translocator (ANT) play key roles in mediating the permeability transition. The contribution of ANT becomes evident in the presence of atractylate (ATR) at pH 6.5, a condition that prevents ATP synthase channel activity. At pH 7.4, this effect is minimal, suggesting a critical role for ATP
Ludovica Tommasin, Andrea Carrer, Federica Boscolo Nata, Elena Frigo, Federico Fogolari, Giovanna Lippe, Michela Carraro, Paolo Bernardi   +7 more
wiley   +1 more source

Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time, isothermal recombinase polymerase amplification assay

, 2015
Infectious hypodermal and hematopoietic necrosis virus (IHHNV) causes mortality or runt deformity syndrome in penaeid shrimps and is responsible for significant economic losses in the shrimp aquaculture industry.
Yu, Yongxin, Wang, Yongjie, Pan, Yingjie, Weidmann, Manfred, Wang, Y., Yan, Shuling, Xia, Xiaoming, Hu, Linghao   +7 more
core   +1 more source

Anatomical and functional mapping of vagal nociceptive sensory nerve subsets innervating the mouse lower airways by intersectional genetics

The Journal of Physiology, EarlyView.
Abstract figure legend We used an intersectional approach with recombinase‐expressing mice and adeno‐associated virus to map and modulate distinct nociceptive afferents in the vagal ganglia. TRPV1+P2X2+ neurons resided in the nodose ganglion (N), innervated the lungs (many projected into the alveoli) but not the trachea, and projected to the nucleus ...
Mayur J. Patil, Justin Shane Hooper, Seol‐Hee Kim, Parmvir K. Bahia, Nika Pavelkova, Sanjay S. Nair, Teresa S. Darcey, Karina V. Lurye, Meghana Madaram, Jailene Fiallo, Stephen H. Hadley, Thomas E. Taylor‐Clark   +11 more
wiley   +1 more source

Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point‐of‐Care Detection of Viral RNA

Angewandte Chemie, Volume 138, Issue 42, 12 October 2026.
A new exponential RCA (E‐RCA) system was developed and coupled to a toehold system for release of a bridging DNA, allowing for lateral flow detection. The E‐RCA method uses a secondary primer (P2) that binds RCA products (RCAP) to generate extended P2 products that can re‐prime a partially self‐complementary circular template, generating a complete ...
Amal Mathai, Monsur Ali, Yingfu Li, John D. Brennan   +3 more
wiley   +2 more sources

Advanced imaging and multi‐omics to characterize cardiomyocyte ageing and its electrocardiographic imprint in PKP2 arrhythmogenic cardiomyopathy

The Journal of Physiology, EarlyView.
Abstract figure legend We used a murine model of cardiomyocyte‐specific conditional knockout of plakophilin 2 (PKP2cKO, middle panel). Using advanced imaging technology, we unveiled distinct hallmarks of premature ageing in PKP2cKO cardiomyocytes, including DNA damage and loss of repressed heterochromatin at the nuclear periphery, and mitochondrial ...
Giorgia Bertoli, Kavya Phadke, Alessandro Cospito, Xianming Lin, Marina Cerrone, Joanna Abi Rizk, Michael Cammer, Yan Deng, Joseph Sall, Mingliang Zhang, Feng‐Xia Liang, Irene de Lázaro, Mario Delmar   +12 more
wiley   +1 more source

Voltage‐gated potassium channels mediate thyroid hormone control of skeletal muscle excitability

The Journal of Physiology, EarlyView.
Abstract figure legend Thyroid hormone (TH)‐dependent remodelling of potassium (K+) channel networks regulates skeletal muscle (SkM) excitability. Triiodothyronine (T3), locally generated from thyroxine (T4) by type 2 deiodinase (D2), binds thyroid hormone receptors (TRα/β) and modulates transcription via thyroid response elements (TREs).
Annarita Nappi, Caterina Miro, Annunziata Gaetana Cicatiello, Ilaria Piccialli, Serena Sagliocchi, Lucia Acampora, Federica Restolfer, Rosa Sirica, Melania Murolo, Emery Di Cicco, Daniela Terracciano, Anna Pannaccione, Monica Dentice   +12 more
wiley   +1 more source

A New Dual Fluorescence Method for Rapid Detection of Infectious Bronchitis Virus at Constant Temperature

Microorganisms
Infectious bronchitis virus (IBV) causes infectious bronchitis in chicken, an acute, highly contagious respiratory infection. Because of genetic mutations and recombination, IBV forms many subtypes, which makes it difficult to treat the disease and apply
Xinheng Zhang, Xiuhong Wu, Keyu Feng, Qian Wang, Qingmei Xie   +4 more
doaj   +1 more source