Results 201 to 210 of about 520,105 (221)
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An automatic centrifugal system for rapid detection of bacteria based on immunomagnetic separation and recombinase aided amplification

Lab on a Chip, 2022
This study reported an automatic centrifugal system for rapid detection of foodborne pathogenic bacteria based on immunomagnetic separation (IMS) for target bacteria enrichment and recombinase aided amplification (RAA) for nucleic acid detection.
Siyuan Wang   +6 more
openaire   +2 more sources

DNA Fragment Fusion and Nucleic Acid Detection by Fusion Recombinase-Aided Amplification

Analytical Chemistry
Constructing fusion DNA fragments is frequently used for genetic engineering purposes. To date, fusion PCR is one of the most popular approaches for generating fusion DNA fragments. Here, we describe a novel method for DNA fusion based on the isothermal DNA amplification technique, recombinase-aided amplification (RAA). We demonstrate that this method,
Xingxing Xiao   +11 more
openaire   +2 more sources

Sensitive detection of multiplex bacteria based on finger driven microfluidics and recombinase aided amplification

Biosensors and Bioelectronics
Foodborne pathogens, such as Salmonella typhimurium, E. coli O157:H7 and Listeria monocytogenes, have seriously threatened human health. To address the issues of traditional bacterial detection methods, such as long time, low sensitivity and complex operation, a versatile finger driven microfluidic chip was elaboratively developed and combined with ...
Jianhan Lin, Yanbin Li, Nana Jin
exaly   +3 more sources

Development of a Recombinase-Aided Amplification Assay for the Rapid Detection of Candida auris

Analytical Chemistry
Candida auris (C. auris) was first discovered in Japan in 2009 and has since spread worldwide. It exhibits strong transmission ability, high multidrug resistance, blood infectivity, and mortality rates. Traditional diagnostic techniques for C. auris have shortcomings, leading to difficulty in its timely diagnosis and identification.
Junxia Feng   +23 more
openaire   +2 more sources

Sensitive Detection of Ralstonia solanacearum Using Recombinase-Aided Amplification and an Aerolysin Nanopore

Langmuir
Ralstonia solanacearum is a soil-borne plant pathogenic bacterium that causes bacterial wilt disease, leading to substantial economic losses in over 250 crops, including tomatoes, tobacco, and potatoes. We developed a novel method for the specific detection of R.
Bin Li   +3 more
openaire   +2 more sources

Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection

Diagnostic Microbiology and Infectious Disease, 2018
In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per ...
Chen, Chen   +7 more
openaire   +2 more sources

Recombinase-Aided Amplification Combined with Lateral Flow Dipstick for the Rapid Detection of Pyrrhoderma noxium

Plant Disease
Pyrrhoderma noxium is an entry quarantine tree pathogenic fungus in China, which causes severe root rot and the death of many tree species, resulting in huge landscape, ecological, and economic losses. P. noxium is a soilborne plant pathogen, which makes it difficult to observe the symptoms of infected trees in the early stage.
Haiyue Yin   +5 more
openaire   +2 more sources

Recombinase aided amplification with photoreactive DNA-binding dye for rapid detection of viable

LWT - Food Science and Technology, 2021
Hengyi Xu, Xiaoyan Feng, Guoyang Xie
exaly  

Development and evaluation of a sensitive recombinase aided amplification assay for rapid detection of

Journal of Microbiological Methods, 2022
Biao Kan, Zhi-Shan Feng, Xin-Xin Shen
exaly  

The fluorescent probe-based recombinase-aided amplification for rapid detection of O157:H7

Molecular and Cellular Probes, 2021
Ju Liu, Hengyi Xu, Guoyang Xie
exaly  

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