Results 71 to 80 of about 520,105 (221)

A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD)

Parasite, 2022
Toxoplasmosis, a parasitic disease resulting from Toxoplasma gondii infection, remains prevalent worldwide, and causes great harm to immunodepressed patients, pregnant women and newborns. Although various molecular approaches to detect T.
Zhao Jinhong, Li Yuanyuan, Xue Qiqi, Zhu Zhiwei, Zou Minghui, Fang Fang   +5 more
doaj   +1 more source

The Natural Product Corramycin Acts as a DNA Gyrase Poison and Overcomes Fluoroquinolone Resistance in Mycobacterium tuberculosis

Advanced Science, EarlyView.
Corramycin, a myxobacterial natural product antibiotic, exhibits potent bactericidal activity against multidrug‐resistant Mycobacterium tuberculosis. The compound hijacks bacterial transporters such as BacA and OppABCD to enter the cell and inhibits DNA synthesis through a previously unrecognized mode of DNA gyrase poisoning, locking the enzyme in an ...
Franziska Fries, Jennifer Herrmann, Michael Dal Molin, Yaëlle Wormser, Timo Risch, Yunsheng Gao, Gareth Prosser, Francesca Gubellini, Ariel Mechaly, Joshua B. Wallach, Anthony Castro, Arka Banerjee, Sari Rasheed, Alexander Horn, Mathias Müsken, Michael Kurz, Lindsay Sonnenkalb, Viola Dreyer, Stefan Niemann, Sönke Andres, Dirk Schnappinger, Uli Kazmaier, Norbert Reiling, Rolf Müller, Jan Rybniker, Stéphanie Petrella   +25 more
wiley   +1 more source

Image_1_A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae.tif

, 2021
Klebsiella pneumoniae carbapenemase genes (blaKPC) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for blaKPC genes and investigations into the molecular characteristics of blaKPC positive Klebsiella ...
Chao Yan (150844), Jing Yuan (217967), Guanhua Xue (416769), Shuheng Du (4033172), Shiyu Liu (1265610), Junxia Feng (8087393), Rongkuan Li (11453755), Lin Gan (114079), Nannan Li (507315), Hanqing Zhao (416771), Juqiang Han (19895), Weiwei Zhang (222964), Jinghua Cui (599162), Wenjian Xu (479291), Rui Zhang (13940), Yanling Feng (294987)   +15 more
core   +1 more source

Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7

Virology Journal, 2019
Background Human adenoviruses are a common group of viruses that cause acute infectious diseases. Human adenovirus (HAdV) 3 and HAdV 7 cause major outbreaks of severe pneumonia.
Rui-huan Wang, Hong Zhang, Yi Zhang, Xin-na Li, Xin-xin Shen, Ju-ju Qi, Guo-hao Fan, Xing-yu Xiang, Zhi-fei Zhan, Zi-wei Chen, Xue-jun Ma   +10 more
doaj   +1 more source

Translational Barriers and AI‐Driven Challenges of Microfluidics‐Enabled Wearables and Implantable Systems in Personalized Medicine

Advanced Science, EarlyView.
An integrative review of microfluidics‐enabled wearables and implantable systems reveals a single‐track translation pipeline, bridging functional biomaterials with clinical utility. Dynamic feedback loops driven by artificial intelligence advance diagnostics toward personalized closed‐loop theranostics.
Ke Huang, Ching Yin Fong, Xin Huang, Bee Luan Khoo   +3 more
wiley   +1 more source

Recombinase polymerase amplification assay for rapid detection of Monkeypox virus [PDF]

, 2019
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed.
Sall, Amadou A., Faye, Ousmane, Davi, Saskia Dede, Kissenkötter, Jonas, Hufert, Frank T, Faye, Martin, Sall, Amadou A, Hufert, Frank T., Böhlken-Fascher, Susanne, Faye, Oumar, Weidmann, Manfred, Ademowo, Olusegun George, Stahl-Hennig, Christiane, Czerny, Claus-Peter, Abd El Wahed, Ahmed   +14 more
core   +1 more source

Comprehensive Dataset for the Development and Validation of a Recombinase Aided Amplification (RAA) Method

This dataset contains the optimization data for primer concentration and reaction conditions of the Recombinase Aided Amplification (RAA) method, as well as the specificity and sensitivity detection data.
卢, 晓漫
core   +2 more sources

A Salmonella Microfluidic Chip Combining Non-Contact Eddy Heater and 3D Fan-Shaped Mixer with Recombinase Aided Amplification

Biosensors, 2022
Foodborne pathogenic bacteria have become a worldwide threat to human health, and rapid and sensitive bacterial detection methods are urgently needed.
Shangyi Wu, Hong Duan, Yingchao Zhang, Siyuan Wang, Lingyan Zheng, Gaozhe Cai, Jianhan Lin, Xiqing Yue   +7 more
doaj   +1 more source

A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints

Advanced Science, EarlyView.
ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu, Jun Yang, Yuhang Zhang, Xiaoli Xue   +3 more
wiley   +1 more source

In Vivo Direct Reprogramming: Current Progress and Future Prospects from Mechanisms to Therapeutic Application

Advanced Science, EarlyView.
This review examines the potential of in vivo direct reprogramming in regenerative medicine for functional tissue restoration, highlighting the role of tissue‐resident cues in generating functionally mature reprogrammed cells from lineage‐related cells. It contains a discussion on mechanisms, reprogramming factors, delivery approaches, and applications
Rishabh Deo Singh, Mauro Calvoli, Kyeong Kyu Kim   +2 more
wiley   +1 more source