Results 81 to 90 of about 520,105 (221)

DNA amplification by Recombinase Polymerase Amplification.

, 2014
The three core proteins, recombinase, single-strand DNA binding protein (SSB) and strand-displacing polymerase enable PCR-like DNA amplification without the need for thermal cycling or an initial chemical or thermal melting step. This diagram was created
David S. Boyle (129165), Jessica C. Meyer (614415), Matthew S. Forrest (71838), Ruth McNerney (227467), Denise M. O'Sullivan (330715), Olaf Piepenburg (60285), Hwee Teng Low (614412), Brandon Troy Leader (614413), David G. Brooks (614416), Ailyn C. Pérez-Osorio (614414)   +9 more
core   +1 more source

DataSheet_1_Rapid Visual Detection of Plasmodium Using Recombinase-Aided Amplification With Lateral Flow Dipstick Assay.doc

, 2022
BackgroundMalaria is a global public health problem. China has had no case of indigenous malaria since 2016. However, imported cases of malaria remain an issue among travelers, overseas workers, and foreign traders.
Lei Shao (227754), Nizhen Jiang (12916763), Yuying Ye (608346), Yanhong Liu (124527), Song Zhao (75272), Hong Lin (46089), Kun Yang (19445)   +6 more
core   +1 more source

Matrix Stiffness Orchestrates Mesenchymal Stem Cell Lineage Commitment Toward Osteogenesis and Adipogenesis Through the PIEZO1/SP1/STC2 Axis

Advanced Science, EarlyView.
Matrix stiffness directs mesenchymal stem cell lineage commitment through PIEZO1‐dependent activation of the SP1/STC2 pathway. Stiff matrices favor osteogenesis and suppress adipogenesis, whereas the loss of PIEZO1 function impairs early bone formation. STC2 retains lineage‐regulatory activity even when PIEZO1 is inactive.
Shuo Zhang, Siteng Li, Wenzhong Chen, Qingcheng Song, Haiyue Zhao, Peng Wang, Yiran Zhang, Wenquan Liang, Shaowei Zheng, Juan Wang, Wei Chen, Yanbin Zhu, Yingze Zhang   +12 more
wiley   +1 more source

The fluorescent probe-based recombinase-aided amplification for rapid detection of Escherichia coli O157:H7

Molecular and Cellular Probes, 2021
Escherichia coli O157:H7 (E. coli O157:H7) is a common foodborne morbigenous microorganism, which can spread through fecal-oral transmission. Humans can be infected by ingesting foods and water contaminated with E. coli O157:H7, which can cause various symptoms.
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Qin Xiong, Xiaoyan Feng, Hengyi Xu   +6 more
openaire   +2 more sources

Development of nucleic acid testing technology for quarantine pest Globodera rostochiensis based on a species-specific DNA fragment [PDF]

PeerJ
Background Globodera rostochiensis, a potato cyst nematode (PCN) species, is distributed in potato-growing regions across Europe, Asia, Africa, Oceania, and the Americas.
Fengqi Lan, Honghao Li, Zhenji Yin, Yajie Wang, Xiaofeng Liu, Jiahui Yang, Yue Sun, Yingqing Tang, Bingjie Wang, Shiyang Yin, Bing Li, Xiang Tao   +11 more
doaj   +2 more sources

A Combination of Novel Nucleic Acid Cross-Linking Dye and Recombinase-Aided Amplification for the Rapid Detection of Viable Salmonella in Milk

Foods, 2022
Salmonella, as an important foodborne pathogen, can cause various diseases, such as severe enteritis. In recent years, various types of nucleicacid-intercalating dyes have been utilized to detect viable Salmonella.
Xiaoyan Feng, Donggen Zhou, Bei Gan, Guoyang Xie, Hengyi Xu   +4 more
doaj   +1 more source

Dual pKa Lipid Nanoparticles for Lung‐tropic mRNA Delivery and pH‐Programmed Endosomal Escape

Advanced Science, EarlyView.
Lipid nanoparticles’ bottleneck lies in low endosomal escape efficiency and liver‐dominant delivery after intravenous administration. To overcome these limitations, we present a stepwise pH‐programmed lipid nanoparticle system enabled by our newly synthesized ionizable lipid.
Seong Gi Lim, Minju Lee, Yiming Wang, Eunjin Choi, Ji Sun Park, Kyungmin Park, Hayoung Jeon, Donghyun Kim, Donghyun Lee, Yeeun Lee, Kee‐Pyo Kim, Eunha Kim, Heebeom Koo   +12 more
wiley   +1 more source

Field-Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae. [PDF]

, 2015
Contagious caprine pleuropneumonia (CCPP) is a highly contagious disease caused by Mycoplasma capricolum subsp. capripneumoniae that affects goats in Africa and Asia.
Nepper, J.F., Weibel, D.B., Mingyan Yu, Heller, M., Younan, M., O'Brien, Elizabeth, Heller, Martin, Yu, Mingyan, Falquet, Laurent, Weibel, Douglas B., Nepper, Julia F., Nepper, Julia F, Gluecks, Ilona, Frey, Joachim, Jores, Joerg, Liljander, Anne, Liljander, Anne M., Frey, J., Weibel, Douglas B, Younan, Mario, Gluecks, Ilona V., O'Brien, E.   +21 more
core   +2 more sources

Prion‐Like Protein LENG8‐Mediated Nucleation Drives Stress Granule Assembly

Advanced Science, EarlyView.
LENG8 is a newly identified stress granule (SG) nucleator required for SG assembly. Following stress, nuclear LENG8 granules disassemble, allowing LENG8 to translocate into the cytoplasm and form independent nucleation foci. These foci fuse with canonical early G3BP1/TIA1 seeds via LENG8‐TIA1 binding to drive SG maturation.
Mingxing Zhang, Xin Wang, Yilan Teng, Zhicheng Wu, Jing Fan, Hongwen Zhu, Peng Dai   +6 more
wiley   +1 more source

Establishment and Application Prospect of Reverse Transcriptase Recombinase-Aided Amplification Assay for Subgroup C Avian Metapneumovirus. [PDF]

Vet Sci
Among broilers, the main pathogen that leads to swollen head syndrome (SHS) is the subgroup C avian metapneumovirus (aMPV-C). The aMPV-C infection can lead to an upsurge in the rate of soft-shell eggs, resulting in reduced egg production and seriously ...
Bai Y, Wu X, Liu J, Wang Z, Dong M, Li T, Dai Z, Li H, Xie Q, Zhang X, Zhang X.   +10 more
europepmc   +2 more sources