Results 91 to 100 of about 131,465 (263)

CRISPR‐Cas9‐Loaded Theranostic Liposomes for Enhancing Radiosensitization of Prostate Cancer through POLD4 Gene Editing under Real‐Time MRI Monitoring

Advanced Science, EarlyView.
This study identifies POLD4 as a potential prostate cancer radiosensitization target through transcriptome sequencing. By encapsulating POLD4‐targeted CRISPR/Cas9 plasmids and USPIONs in cationic liposomes, an MRI‐monitored gene‐editing platform is established.
Xuhui Fan, Ruru Zhang, Linjun Yang, Shixiong Chen, Meijuan He, Yongqiang Wang, Linjie Huang, Jianfeng Zeng, Shuwang Wu, Mingyuan Gao, Han Wang   +10 more
wiley   +1 more source

Recombinase Polymerase Amplification-Based Assay to Diagnose Giardia in Stool Samples [PDF]

The American Society of Tropical Medicine and Hygiene, 2015
Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG
Crannell, Zachary Austin, Cabada, Miguel Mauricio, Castellanos-Gonzalez, Alejandro, Irani, Ayesha, White, Arthur Clinton, Richards-Kortum, Rebecca   +5 more
openaire   +3 more sources

Rapid detection of mexX in Pseudomonas aeruginosa based on CRISPR-Cas13a coupled with recombinase polymerase amplification

Frontiers in Microbiology
The principal pathogen responsible for chronic urinary tract infections, immunocompromised hosts, and cystic fibrosis patients is Pseudomonas aeruginosa, which is difficult to eradicate.
Xiao-xuan Zhu, Ying-si Wang, Su-juan Li, Ru-qun Peng, Xia Wen, H. Peng, Qing-Shan Shi, Gang Zhou, Xiaobao Xie, Jie Wang   +9 more
semanticscholar   +1 more source

Astrocytic TIA1‐Mediated Stress Granules Promote the Demyelination of Optic Neuritis by Sequestering mRNA of Cholesterol Synthesis Genes in an Experimental Autoimmune Encephalomyelitis Model

Advanced Science, EarlyView.
Astrocytic TIA1‐mediated stress granules (SGs) promote demyelination by sequestering the mRNA of cholesterol synthesis genes. In this model, astrocytic TIA1‐mediated SGs were increased in the optic nerves of EAE mice, leading to the downregulation of cholesterol synthesis genes such as HMGCS1 through sequestration of their mRNA into SGs, which ...
Zheyu Fang, Xin Hua, Ziwei Fan, Jiaxin Zhao, Yuan Zhang, Qiaoyi Zhang, Xi Qu, Shengli Pan, Bingbing Wan, Shiyin Yang, Jia Li, Xu Zhang   +11 more
wiley   +1 more source

Extensive horizontal gene transfer in cheese-associated bacteria. [PDF]

, 2017
Acquisition of genes through horizontal gene transfer (HGT) allows microbes to rapidly gain new capabilities and adapt to new or changing environments.
Bonham, Kevin S, Dutton, Rachel J, Wolfe, Benjamin E   +2 more
core   +3 more sources

Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for the Rapid and Sensitive Detection of Pseudo-nitzschia multiseries

International Journal of Molecular Sciences
The harmful algal bloom (HAB) species Pseudo-nitzschia multiseries is widely distributed worldwide and is known to produce the neurotoxin domoic acid, which harms marine wildlife and humans.
Yuqing Yao, Ningjian Luo, Yujie Zong, Meng-ao Jia, Yichen Rao, Hailong Huang, Haibo Jiang   +6 more
semanticscholar   +1 more source

Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity

Advanced Science, EarlyView.
Most rare diseases are caused by genetic mutations. Prime editing (PE) has emerged as a versatile tool capable of inducing diverse mutations without generating DNA double‐strand breaks. Despite its significant clinical potential, PE faces limitations in terms of efficiency and scalability.
Jihyeon Yu, Ju‐Chan Park, Heesoo Uhm, Yong‐Woo Kim, Hyeon Woo Im, Sangsu Bae   +5 more
wiley   +1 more source

A recombinase polymerase amplification and Pyrococcus furiosus Argonaute combined method for ultra-sensitive detection of white spot syndrome virus in shrimp.

Journal of Fish Diseases, 2023
White spot disease (WSD) in shrimp is an acute infectious disease caused by white spot syndrome virus (WSSV). WSD has seriously threatened the security of shrimp farming, causing huge economic losses worldwide.
Yu Wang, Yukang Chen, Yixin Tang, Yue-Heng Wang, Song Gao, Lihong Yang, Pei Wang   +6 more
semanticscholar   +1 more source

A Single‐Enzyme Activated CRISPR‐Cas12a Nano System via Subtly Balanced dsDNA for Kinetic‐Gated UDG Detection and Spatiotemporal Cellular Imaging

Advanced Science, EarlyView.
Kinetic‐gated CRISPR–Cas12a activation enables single‐enzyme detection and spatiotemporal imaging of UDG ABSTRACT Uracil‐DNA glycosylase (UDG) is a key enzyme in base excision repair and an important biomarker for genomic stability and disease. In many reported sensing systems, uracil excision is coupled to signal generation through additional ...
Kejun Dong, Hao Hu, Haiyun Wang, Zijia Zheng, Shuangshuang Cheng, Wan Shu, Ruisi Liu, Xiaoyan Xin, Shiyi Huang, Dingchao Qian, Xianjin Xiao, Qiang Fu, Hongbo Wang   +12 more
wiley   +1 more source

CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. [PDF]

, 2017
Broad-scale protein-protein interaction mapping is a major challenge given the cost, time, and sensitivity constraints of existing technologies. Here, we present a massively multiplexed yeast two-hybrid method, CrY2H-seq, which uses a Cre recombinase ...
Bartlett, Anna, Castanon, Rosa, Ecker, Joseph R, Feeney, Joseph, Galli, Mary, Garza, Renee M, Goubil, Adeline, Huang, Shao-Shan C, MacWilliams, Andrew, Nery, Joseph R, O'Malley, Ronan, Trigg, Shelly A, Zhang, Zhuzhu Z   +12 more
core   +2 more sources