Results 71 to 80 of about 131,465 (263)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

The 10–23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions

Angewandte Chemie, EarlyView.
This review focuses on the 10–23 DNAzyme in diagnostics, spanning unregulated and regulated target‐recognition modes, and their functionalization across colorimetric, fluorescent, electrochemical, electrochemiluminescent, and intracellular biosensing strategies.
Connor Nurmi, Jake Brill, Sanne Roumans, Amal Mathai, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems. [PDF]

3 Biotech
Anbazhagan P, Parameswari B, Anitha K, Chaitra GV, Bajaru B, Rajashree A, Mangrauthia SK, Yousuf F, Chalam VC, Singh GP.   +9 more
europepmc   +2 more sources

Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification [PDF]

, 2015
Yam mosaic virus (YMV; genus Potyvirus) is considered to cause the most economically important viral disease of yams (Dioscorea spp.) in West Africa which is the dominant region for yam production globally.
Abarshi, Abd El Wahed, Aleman, Aleman-Verdaguer, Amer, Ampofo, Asala, Asiedu, Bousalem, Boyle, Chukwuemeka Nkere, Craw, Eni, Eni, Eni, Escadafal, Euler, Euler, Gonçalo Silva, Kenyon, Kenyon, Lillis, Mambole, Maroya, Mekuria, Menzel, Moritz Bömer, Mumford, Njukeng, Njukeng, Notomi, P. Lava Kumar, Piepenburg, Seal, Susan E. Seal, Teoh, Thouvenel, Xia, Zanoli, Zhang   +39 more
core   +1 more source

Magnetic concentration of allele-specific products from recombinase polymerase amplification [PDF]

Analytica Chimica Acta, 2019
The studied challenge is the specific detection of low-abundant genomic variants that differ by a single nucleotide from the wild type. The combination of blocked recombinase polymerase amplification (RPA) and selective capture by probes immobilised on magnetic-core particles integrated into a flow system is presented.
Sara Martorell, Luis A. Tortajada-Genaro, Ángel Maquieira   +2 more
openaire   +4 more sources

Liver Stiffness Directs Intrahepatic Cholesterol Accumulation Through YAP/TAZ in Metabolic Dysfunction‐Associated Steatotic Liver Disease

Advanced Science, EarlyView.
Liver stiffness promotes intrahepatic cholesterol accumulation by repressing LXRα through YAP/TAZ activation. Stiff matrices impair cholesterol efflux in hepatocytes, while YAP/TAZ deletion restores LXRα activity and prevents cholesterol‐induced fibrosis.
Na Young Lee, Myeung Gi Choi, Ho Jae Ryu, Young Jin Min, Seon Min Kim, Yeonseok Chung, Bo Kyung Koo, Yun Pyo Kang, Won Kim, Ja Hyun Koo   +9 more
wiley   +1 more source

Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases

Журнал микробиологии, эпидемиологии и иммунобиологии
Isothermal amplification techniques have been actively developed in recent years and are gradually introduced into the range of methods for infectious disease diagnostics. One of the fastest isothermal methods is recombinase polymerase amplification (RPA)
Olga S. Bondareva, Artem A. Baturin, Anna V. Mironova   +2 more
doaj   +1 more source

Testicular mRNA‐LNP Delivery: A Novel Therapy for Genetic Spermatogenic Disorders

Advanced Science, EarlyView.
In the current study, we developed a novel therapy for genetic spermatogenic disorders. It was revealed that in vivo delivery of mRNA LNP3 could restore spermatogenesis in Msh5D486Y/D486Y and Maps KO mouse models with meiotic arrest. Notably, the offspring without genomic integration was born using ICSI derived from the rescue of Msh5D486Y/D486Y mouse ...
Chenwang Zhang, Nan Liang, Wenbo Li, Shuai Xu, Peng Li, Wanze Ni, Na Li, Sha Han, Ningjing Ou, Haowei Bai, Yuxiang Zhang, Furong Bai, Yifan Sun, Dewei Qian, Xinjie Bu, Erlei Zhi, Ruhui Tian, Yuhua Huang, Jingpeng Zhao, Fujun Zhao, Hao Chen, Zheng Li, Chencheng Yao   +22 more
wiley   +1 more source

Sensitive, Accurate and Rapid Detection of the Northern Root-Knot Nematode, Meloidogyne hapla, Using Recombinase Polymerase Amplification Assays

Plants, 2021
Rapid and reliable diagnostics of root-knot nematodes are critical for selections of effective control against these agricultural pests. In this study, recombinase polymerase amplification (RPA) assays were developed targeting the IGS rRNA gene of the ...
Sergei A. Subbotin, Julie Burbridge
doaj   +1 more source

Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes. [PDF]

, 2012
BAckground: Massively parallel sequencing technology is revolutionizing approaches to genomic and genetic research. Since its advent, the scale and efficiency of Next-Generation Sequencing (NGS) has rapidly improved. In spite of this success, sequencing
Campino, Susana, Gu, Yong, Kwiatkowski, Dominic P, Macinnis, Bronwyn, Manske, Magnus, Maslen, Gareth, Otto, Thomas D, Oyola, Samuel O, Quail, Michael A, Swerdlow, Harold P, Turner, Daniel J   +10 more
core