Results 1 to 10 of about 14,888 (293)

RECC: A Red/ET–CRISPR/Cas9-based system enabling genome mining of marine Pseudoalteromonas for novel natural products [PDF]

Synthetic and Systems Biotechnology
Marine microorganisms possess vast biosynthetic potential, yet most of their biosynthetic gene clusters (BGCs) remain transcriptionally silent under laboratory conditions.
Zong-jie Wang, Haibo Zhou, Youming Zhang, Fu Yan, Liujie Huo, Xiaotong Wang   +5 more
doaj   +2 more sources

Conversion of a telomere resolvase into a Cre-like site-specific recombinase. [PDF]

PLoS One
Huang SH, Balouchi M, Kobryn K.
europepmc   +2 more sources

Redesigning Recombinase Specificity for Safe Harbor Sites in the Human Genome. [PDF]

PLoS ONE, 2015
Site-specific recombinases (SSRs) are valuable tools for genetic engineering due to their ability to manipulate DNA in a highly specific manner. Engineered zinc-finger and TAL effector recombinases, in particular, are two classes of SSRs composed of ...
Mark C Wallen, Thomas Gaj, Carlos F Barbas   +2 more
doaj   +1 more source

Computational characterization of recombinase circuits for periodic behaviors

iScience, 2023
Summary: Recombinases are site-specific proteins found in nature that are capable of rearranging DNA. This function has made them promising gene editing tools in synthetic biology, as well as key elements in complex artificial gene circuits implementing ...
Judith Landau, Christian Cuba Samaniego, Giulia Giordano, Elisa Franco   +3 more
doaj   +1 more source

Prediction of designer-recombinases for DNA editing with generative deep learning

Nature Communications, 2022
Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholz   +3 more
doaj   +1 more source

Temperate phages acquire DNA from defective prophages by relaxed homologous recombination: the role of Rad52-like recombinases. [PDF]

PLoS Genetics, 2014
Bacteriophages (or phages) dominate the biosphere both numerically and in terms of genetic diversity. In particular, genomic comparisons suggest a remarkable level of horizontal gene transfer among temperate phages, favoring a high evolution rate ...
Marianne De Paepe, Geoffrey Hutinet, Olivier Son, Jihane Amarir-Bouhram, Sophie Schbath, Marie-Agnès Petit   +5 more
doaj   +1 more source

Site‐specific recombinase genome engineering toolkit in maize

Plant Direct, 2020
Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, reactions result in
Jon P. Cody, Nathaniel D. Graham, Changzeng Zhao, Nathan C. Swyers, James A. Birchler   +4 more
doaj   +1 more source

Two Subpopulations of Noradrenergic Neurons in the Locus Coeruleus Complex Distinguished by Expression of the Dorsal Neural Tube Marker Pax7

Frontiers in Neuroanatomy, 2017
Central noradrenergic neurons, collectively defined by synthesis of the neurotransmitter norepinephrine, are a diverse collection of cells in the hindbrain, differing in their anatomy, physiological and behavioral functions, and susceptibility to disease
Nicholas W. Plummer, Erica L. Scappini, Kathleen G. Smith, Charles J. Tucker, Patricia Jensen   +4 more
doaj   +1 more source

Stimulation of homologous recombination in plants expressing heterologous recombinases

BMC Plant Biology, 2020
Background Current excitement about the opportunities for gene editing in plants have been prompted by advances in CRISPR/Cas and TALEN technologies. CRISPR/Cas is widely used to knock-out or modify genes by inducing targeted double-strand breaks (DSBs ...
Abdellah Barakate, Ewan Keir, Helena Oakey, Claire Halpin   +3 more
doaj   +1 more source

Computational Insights into the Dynamic Structural Features and Binding Characteristics of Recombinase UvsX Compared with RecA

Molecules, 2023
RecA family recombinases are the core enzymes in the process of homologous recombination, and their normal operation ensures the stability of the genome and the healthy development of organisms.
Yue Pan, Ningkang Xie, Xin Zhang, Shuo Yang, Shaowu Lv   +4 more
doaj   +1 more source