Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Recombination-mediated host-adaptation by avian Staphylococcus aureus
, 2016 Supplementary tables for 'Recombination-mediated host-adaptation by avian Staphylococcus aureus' by Murray et al. Figure S1: Genetic relatedness of S.
Ben Pascoe (3139548)
core +1 more source
federlab/hiv-recombination: v1.0.0 - BioRxiv submission
, 2023 Associated code for Elevated HIV viral load is associated with higher recombination rate in vivo Elena Romero @evromero-uw Alison Feder @affeder https://doi.org/10.1101/2023.05.05 ...
evromero-uw, affeder
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Comparative gene mapping in Arabidopsis lyrata chromosomes 6 and 7 and A thaliana chromosome IV: evolutionary history, rearrangements and local recombination rates [PDF]
, 2006 We have increased the density of genetic markers on the Arabidopsis lyrata chromosomes AL6 and AL7 corresponding to the A. thaliana chromosome IV, in order to determine chromosome rearrangements between these two species, and to compare recombination ...
Hansson, Bengt +9 more
core +1 more source
An impact of physical phenomena on admittances of partially-depleted SOI MOSFETs
Journal of Telecommunications and Information Technology, 2001 An in uence of the selected physical phenom- ena: impact ionization in silicon and time variation of inter- nal electric field distribution in partially-depleted (PD) SOI MOSFETs on several C-V characteristics of these devices is presented.
Jan Gibki
doaj +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells [PDF]
, 2005 Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström +16 more
core +1 more source
ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function
FEBS Open Bio, EarlyView.Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley +5 more
wiley +1 more source
Genomic analysis of meiotic recombination in social insects [PDF]
, 2022 Meiotic recombination is a key event of sexual reproduction in higher eukaryotes that shuffles allele combinations and promotes accurate segregation of chromosomes. The rate of meiotic recombination varies within and across species.
Waiker, Prashant +1 more
core