Results 21 to 30 of about 1,765,900 (302)

Reference Gene Screening for Analyzing Gene Expression Across Goat Tissue [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2013
Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the changes in mRNA expression levels in cells and tissues. Selection of the proper reference genes is very important when calibrating the results of real-time quantitative PCR.
Yu Zhang   +6 more
openaire   +3 more sources

Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Trifolium repens L.) Involved in Five Abiotic Stresses

open access: yesPlants, 2020
White clover (Trifolium repens L.) is a widely cultivated cool-season perennial forage legume in temperate grassland systems. Many studies have analyzed the gene expression in this grass species using quantitative real-time reverse transcription PCR (qRT-
Qi Pu   +5 more
doaj   +1 more source

I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues in Arabidopsis thaliana

open access: yesBiomolecules, 2023
Quantitative real-time polymerase chain reaction (qPCR) is a widely used method to analyse the gene expression pattern in the reproductive tissues along with detecting gene levels in mutant backgrounds.
Maria João Ferreira   +3 more
doaj   +1 more source

New Anti-inflammatory Cyclopeptides From a Sponge-Derived Fungus Aspergillus violaceofuscus

open access: yesFrontiers in Chemistry, 2018
Three new cyclic peptides including a cyclic tetrapeptide (1), an aspochracin-type cyclic tripeptide sclerotiotide L (2) and a diketopiperazine dimer (3), have been isolated from the ethyl acetate extract of a marine sponge-derived fungus Aspergillus ...
Jingtang Liu   +4 more
doaj   +1 more source

Validation of suitable internal control genes for expression studies in aging. [PDF]

open access: yes, 2009
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA   +9 more
core   +1 more source

Validation of Reference Genes in a Population of Blueberry (Vaccinium corymbosum) Plants Regenerated in Colchicine

open access: yesPlants, 2022
For the first time we report the validation of reference genes in plants from a population of blueberry (Vaccinium corymbosum) clones cultured in vitro on a colchicine-supplemented medium.
Francisca Valenzuela   +4 more
doaj   +1 more source

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

open access: yes, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra   +6 more
core   +2 more sources

miR-103a-3p and miR-22-5p Are Reliable Reference Genes in Extracellular Vesicles From Cartilage, Adipose Tissue, and Bone Marrow Cells

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Cartilage cells (CCs), adipose tissue (ASC)- and bone marrow (BMSC)-derived mesenchymal stromal cells (MSCs) have been shown as promising candidates for the treatment of osteoarthritis (OA).
Enrico Ragni   +7 more
doaj   +1 more source

Transcriptome analyses reveal reduced hepatic lipid synthesis and accumulation in more feed efficient beef cattle [PDF]

open access: yes, 2018
peer-reviewedThe genetic mechanisms controlling residual feed intake (RFI) in beef cattle are still largely unknown. Here we performed whole transcriptome analyses to identify differentially expressed (DE) genes and their functional roles in liver ...
Fitzsimmons, Carolyn   +6 more
core   +3 more sources

Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens

open access: yesScientific Reports, 2022
AbstractMicroalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to the colonization of terrestrial life.
Chatelain, Pauline   +6 more
openaire   +5 more sources

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