Results 41 to 50 of about 6,662,733 (309)

Epidermal reference genes at the forefront of data interpretation [PDF]

Experimental Dermatology, 2015
The characterization of gene expression, its identification and its measurement, is essential for understanding the somewhat unique fate of keratinocytes, their normal functions, but also the behavior of this cell type in skin diseases. Normal keratinocytes first proliferate under strict control before being committed towards a progressive program of ...
Abdallah Mound, Jérémy Malaisse, Évelyne De Vuyst, Aurélie Hayez, Catherine Lambert de Rouvroit, Mark R. Pittelkow, Yves Poumay   +6 more
openaire   +3 more sources

Selection and Validation of Reference Genes for qRT-PCR Analysis of Gene Expression in Tropaeolum majus (Nasturtium)

Horticulturae, 2023
Tropaeolum majus (nasturtium) is an important ornamental and medicinal plant due to its colorful flowers, shield-shaped leaves, and richness in mineral elements and bioactive compounds. However, the key genes related to these important biological traits,
Qing Tang, Guang-Can Zhou, Si-Jie Liu, Wen Li, Yi-Lei Wang, Gao-Ying Xu, Teng-Fei Li, Guo-Qing Meng, Jia-Yu Xue   +8 more
doaj   +1 more source

Approach for defining endogenous reference genes in gene expression experiments

Analytical Biochemistry, 2004
The quantification of gene expression by real-time polymerase chain reaction (PCR) has revolutionized the field of gene expression analysis. Due to its sensitivity and flexibility it is becoming the method of choice for many investigators. However, good normalization protocols still have to be implemented to facilitate data exchange and comparison.
Garcia Vallejo, J.J., Van Het Hof, B, Robben, J, Van Wijk, JA, van Die, I.M., Joziasse, D.H., van Dijk, W.   +6 more
openaire   +3 more sources

Evaluation of reference genes for insect olfaction studies [PDF]

Parasites & Vectors, 2015
La PCR cuantitativa de transcripción inversa (qRT-PCR) es un método robusto y accesible para analizar la expresión génica e inferir la regulación génica. Al ser una cadena de procedimientos, esta técnica está sujeta a errores sistemáticos debido a limitaciones biológicas y técnicas establecidas principalmente por el material de partida y los ...
Bonaventure Aman Omondi, José Manuel Latorre-Estivalis, Ivana Helena Rocha Oliveira, Rickard Ignell, Marcelo Gustavo Lorenzo   +4 more
openaire   +4 more sources

Selection and Validation of Reference Genes for Normalisation of Gene Expression in Glehnia littoralis [PDF]

Scientific Reports, 2020
AbstractGlehnia littoralisis an important medicinal halophyte—the dried root of which is used as Chinese herbal medicine. However, the use, selection and stability of reference genes are rarely verified in studies ofG. littoralis, which hampers investigation of its salt tolerance and metabolism.
Li, Li, Li, Naiwei, Fang, Hailing, Qi, Xiwu, Zhou, Yifeng   +4 more
openaire   +2 more sources

A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression

Forestry Research
Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) plays a crucial role in relative gene expression analysis, and accurate normalization relies on suitable reference genes (RGs).
Qi Xie, Umair Ahmed, Cheng Qi, Kebing Du, Jie Luo, Pengcheng Wang, Bo Zheng, Xueping Shi   +7 more
doaj   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Establishing Reference Genes for Accurate Gene Expression Profiling in Toxigenic Bacillus cereus

Toxins
Bacillus cereus is a Gram-positive pathogen associated with foodborne illnesses and severe non-gastrointestinal infections. Robust tools for accurate gene transcription analysis are essential for studying toxin gene expression dynamics and deciphering ...
Tanja V. Edelbacher, Astrid Laimer-Digruber, Michael W. Pfaffl, Monika Ehling-Schulz   +3 more
doaj   +1 more source

Assessment of Suitable Reference Genes for RT-qPCR Normalization with Developmental Samples in Pacific Abalone Haliotis discus hannai

Journal of Animal Reproduction and Biotechnology, 2019
Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different ...
Sang Yoon Lee, Choul-Ji Park, Yoon Kwon Nam   +2 more
doaj   +1 more source

Optimal Reference Genes for Gene Expression Normalization in Trichomonas vaginalis

PLOS ONE, 2015
Trichomonas vaginalis is the etiologic agent of trichomonosis, the most common non-viral sexually transmitted disease worldwide. This infection is associated with several health consequences, including cervical and prostate cancers and HIV acquisition.
Odelta dos Santos, Graziela de Vargas Rigo, Amanda Piccoli Frasson, Alexandre José Macedo, Tiana Tasca   +4 more
openaire   +4 more sources