Results 41 to 50 of about 6,952,897 (310)

Universal RNA Reference Materials for Gene Expression [PDF]

Clinical Chemistry, 2004
Abstract A workshop entitled “Metrology and Standards Needs for Gene Expression Technologies: Universal RNA Standards” was held in March 2003 to define the requirements for standardizing RNA-based molecular assays, specifically microarray and quantitative reverse-transcriptase-PCR technologies.
Maureen, Cronin, Krishna, Ghosh, Frank, Sistare, John, Quackenbush, Vincent, Vilker, Catherine, O'Connell   +5 more
openaire   +2 more sources

Selection and Validation of Reference Genes for qRT-PCR Analysis of Gene Expression in Tropaeolum majus (Nasturtium)

Horticulturae, 2023
Tropaeolum majus (nasturtium) is an important ornamental and medicinal plant due to its colorful flowers, shield-shaped leaves, and richness in mineral elements and bioactive compounds. However, the key genes related to these important biological traits,
Qing Tang, Guang-Can Zhou, Si-Jie Liu, Wen Li, Yi-Lei Wang, Gao-Ying Xu, Teng-Fei Li, Guo-Qing Meng, Jia-Yu Xue   +8 more
doaj   +1 more source

Candidate Reference Genes.

, 2013
Candidate Reference Genes.
Jocelyn M. Wessels (337687), Andrew K. Edwards (337689), Chandrakant Tayade (337692), Candace Zettler (337690)   +3 more
core   +1 more source

Evaluation of reference genes for insect olfaction studies [PDF]

Parasites & Vectors, 2015
La PCR cuantitativa de transcripción inversa (qRT-PCR) es un método robusto y accesible para analizar la expresión génica e inferir la regulación génica. Al ser una cadena de procedimientos, esta técnica está sujeta a errores sistemáticos debido a limitaciones biológicas y técnicas establecidas principalmente por el material de partida y los ...
Bonaventure Aman Omondi, José Manuel Latorre-Estivalis, Ivana Helena Rocha Oliveira, Rickard Ignell, Marcelo Gustavo Lorenzo   +4 more
openaire   +4 more sources

Candidate reference genes.

, 2014
Candidate reference genes.
Miguel Such-Martínez (566227), Borja Fernández (566228), Manuel Jiménez-Navarro (566226), María José Mataró (566223), Juan Robledo-Carmona (566224), Oscar Lamas (462463), Carmen Rueda-Martínez (566222), Gemma Sánchez-Espín (566225)   +7 more
core   +1 more source

Analysis of reference genes by BestKeeper algorithm.

, 2023
Analysis of reference genes by BestKeeper algorithm.
Shehla Izhar (16898619), Erica Acton (4027031), Andrew Baker (555033), Amanda Formosa (13273585), Pamela Plant (2621731), Jim N. Tsoporis (16898622), Paul Turgeon (13273588), Amy Lee (847764), Claudia C. dos Santos (9081415), Uriel Trahtemberg (182469), Sabri Soussi (3412004)   +10 more
core   +1 more source

Approach for defining endogenous reference genes in gene expression experiments

Analytical Biochemistry, 2004
The quantification of gene expression by real-time polymerase chain reaction (PCR) has revolutionized the field of gene expression analysis. Due to its sensitivity and flexibility it is becoming the method of choice for many investigators. However, good normalization protocols still have to be implemented to facilitate data exchange and comparison.
Garcia Vallejo, J.J., Van Het Hof, B, Robben, J, Van Wijk, JA, van Die, I.M., Joziasse, D.H., van Dijk, W.   +6 more
openaire   +3 more sources

Putative reference genes evaluated.

, 2013
Putative reference genes evaluated.
Barbara Żyżyńska-Granica (295244), Katarzyna Koziak (295245)   +1 more
core   +1 more source

A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression

Forestry Research
Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) plays a crucial role in relative gene expression analysis, and accurate normalization relies on suitable reference genes (RGs).
Qi Xie, Umair Ahmed, Cheng Qi, Kebing Du, Jie Luo, Pengcheng Wang, Bo Zheng, Xueping Shi   +7 more
doaj   +1 more source

Assessment of Suitable Reference Genes for RT-qPCR Normalization with Developmental Samples in Pacific Abalone Haliotis discus hannai

Journal of Animal Reproduction and Biotechnology, 2019
Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different ...
Sang Yoon Lee, Choul-Ji Park, Yoon Kwon Nam   +2 more
doaj   +1 more source