Results 31 to 40 of about 6,662,733 (309)

Validation of Reliable Reference Genes for RT-qPCR Studies of Target Gene Expression in Colletotrichum camelliae During Spore Germination and Mycelial Growth and Interaction With Host Plants

Frontiers in Microbiology, 2019
The tea plant [Camellia sinensis (L.) O. Kuntze] is one of the most important leaf crops, and it is widely used for the production of non-alcoholic beverages worldwide. Tea also has a long history of medicinal use.
Shengnan He, Tai An, Runa A, Shouan Liu
doaj   +1 more source

Selection of reference genes for expression studies with fish myogenic cell cultures [PDF]

, 2009
Background: Relatively few studies have used cell culture systems to investigate gene expression and the regulation of myogenesis in fish. To produce robust data from quantitative real-time PCR mRNA levels need to be normalised using internal reference ...
Bower, Neil I., Neil I Bower, Ian A Johnston, Johnston, Ian A.   +3 more
core   +1 more source

A reference gene catalogue of the pig gut microbiome [PDF]

Nature Microbiology, 2016
The pig is a major species for livestock production and is also extensively used as the preferred model species for analyses of a wide range of human physiological functions and diseases1. The importance of the gut microbiota in complementing the physiology and genome of the host is now well recognized2.
Liang Xiao, Jordi Estellé, Pia Kiilerich, Yuliaxis Ramayo-Caldas, Zhongkui Xia, Qiang Feng, Suisha Liang, Anni Øyan Pedersen, Niels Jørgen Kjeldsen, Chuan Liu, Emmanuelle Maguin, Joël Doré, Nicolas Pons, Emmanuelle Le Chatelier, Edi Prifti, Junhua Li, Huijue Jia, Xin Liu, Xun Xu, Stanislav D. Ehrlich, Lise Madsen, Karsten Kristiansen, Claire Rogel-Gaillard, Jun Wang   +23 more
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Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Normalization in Staminate and Perfect Flowers of Andromonoecious Taihangia rupestris

Frontiers in Plant Science, 2017
Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is the most commonly used and powerful method for gene expression analysis due to its high sensitivity, specificity, and high throughput, and the accuracy of this approach ...
Weiguo Li, Weiguo Li, Lihui Zhang, Yandi Zhang, Guodong Wang, Dangyu Song, Yanwen Zhang   +6 more
doaj   +1 more source

Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples

Frontiers in Genetics, 2019
Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC.
Hui Wang, Yuan Lv, Cao Wang, Dongjing Leng, Yan Yan, Moyondafoluwa Blessing Fasae, Syeda Madiha Zahra, Yanan Jiang, Yanan Jiang, Zhiguo Wang, Baofeng Yang, Baofeng Yang, Yunlong Bai, Yunlong Bai   +13 more
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Reference-based QUantification Of gene Dispensability (QUOD) [PDF]

Plant Methods, 2020
Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level.
Frey, Katharina, Weisshaar, Bernd, Pucker, Boas   +2 more
openaire   +4 more sources

Assessment of the specificity and stability of Micro-RNAs as a forensic gene marker

Biomedical and Biotechnology Research Journal, 2023
Background: Forensic investigations depend on bodily fluid analysis to identify the perpetrators. Identifying perpetrators requires knowledge about suspects' body fluids.
Yasir Haider Al-Mawlah, Asma'a H Mohamed, Ali Mohammad Abd-Alameer, Ameer Mezher Hadi, Hadi Sajid Abdulabbas, Salah Hashim Shaheed, Mohammed Abdullah Jebor, Ali Hmood Alsaadi   +7 more
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Validation of reference genes in human chordoma

Surgical Neurology International, 2017
Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference ...
Santegoeds, R G C, Yakkioui, Y, Jahanshahi, A, Hoogland, G, Temel, Y, van Overbeeke, J J   +5 more
openaire   +3 more sources

Screening and Validation of Reference Genes for RT-qPCR Under Different Honey Bee Viral Infections and dsRNA Treatment

Frontiers in Microbiology, 2020
Honey bee viruses are one of the most important pathogens that have contributed to the decrease in honey bee colony health. To analyze the infection dynamics of honey bee viruses, quantification of viral gene expression by RT-qPCR is necessary.
Yanchun Deng, Yanchun Deng, Yanchun Deng, Hongxia Zhao, Sa Yang, Sa Yang, Li Zhang, Li Zhang, Lina Zhang, Lina Zhang, Chunsheng Hou, Chunsheng Hou   +11 more
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Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source