Results 31 to 40 of about 6,952,897 (310)

Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Normalization in Staminate and Perfect Flowers of Andromonoecious Taihangia rupestris

Frontiers in Plant Science, 2017
Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is the most commonly used and powerful method for gene expression analysis due to its high sensitivity, specificity, and high throughput, and the accuracy of this approach ...
Weiguo Li, Weiguo Li, Lihui Zhang, Yandi Zhang, Guodong Wang, Dangyu Song, Yanwen Zhang   +6 more
doaj   +1 more source

Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples

Frontiers in Genetics, 2019
Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC.
Hui Wang, Yuan Lv, Cao Wang, Dongjing Leng, Yan Yan, Moyondafoluwa Blessing Fasae, Syeda Madiha Zahra, Yanan Jiang, Yanan Jiang, Zhiguo Wang, Baofeng Yang, Baofeng Yang, Yunlong Bai, Yunlong Bai   +13 more
doaj   +1 more source

Screening and Validation of Reference Genes for RT-qPCR Under Different Honey Bee Viral Infections and dsRNA Treatment

Frontiers in Microbiology, 2020
Honey bee viruses are one of the most important pathogens that have contributed to the decrease in honey bee colony health. To analyze the infection dynamics of honey bee viruses, quantification of viral gene expression by RT-qPCR is necessary.
Yanchun Deng, Yanchun Deng, Yanchun Deng, Hongxia Zhao, Sa Yang, Sa Yang, Li Zhang, Li Zhang, Lina Zhang, Lina Zhang, Chunsheng Hou, Chunsheng Hou   +11 more
doaj   +1 more source

Assessment of the specificity and stability of Micro-RNAs as a forensic gene marker

Biomedical and Biotechnology Research Journal, 2023
Background: Forensic investigations depend on bodily fluid analysis to identify the perpetrators. Identifying perpetrators requires knowledge about suspects' body fluids.
Yasir Haider Al-Mawlah, Asma'a H Mohamed, Ali Mohammad Abd-Alameer, Ameer Mezher Hadi, Hadi Sajid Abdulabbas, Salah Hashim Shaheed, Mohammed Abdullah Jebor, Ali Hmood Alsaadi   +7 more
doaj   +1 more source

Validation of reference genes in human chordoma

Surgical Neurology International, 2017
Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference ...
Santegoeds, R G C, Yakkioui, Y, Jahanshahi, A, Hoogland, G, Temel, Y, van Overbeeke, J J   +5 more
openaire   +3 more sources

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source

Epidermal reference genes at the forefront of data interpretation [PDF]

Experimental Dermatology, 2015
The characterization of gene expression, its identification and its measurement, is essential for understanding the somewhat unique fate of keratinocytes, their normal functions, but also the behavior of this cell type in skin diseases. Normal keratinocytes first proliferate under strict control before being committed towards a progressive program of ...
Abdallah Mound, Jérémy Malaisse, Évelyne De Vuyst, Aurélie Hayez, Catherine Lambert de Rouvroit, Mark R. Pittelkow, Yves Poumay   +6 more
openaire   +3 more sources

Reference-based QUantification Of gene Dispensability (QUOD) [PDF]

Plant Methods, 2020
Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level.
Frey, Katharina, Weisshaar, Bernd, Pucker, Boas   +2 more
openaire   +4 more sources

Candidate reference genes.

, 2016
Candidate reference genes.
Helena J. Maier (3043422), Mark Fife (3043425), Ambalika Batra (3043431), William Mwangi (3043428), Colin Butter (104550), John R. Young (104544), Karen Staines (104539), Steven Van Borm (313768)   +7 more
core   +1 more source

Screening of Suitable Reference Genes for Immune Gene Expression Analysis Stimulated by Vibrio anguillarum and Copper Ions in Chinese Mitten Crab (Eriocheir sinensis)

, 2023
The reference gene expression is not always stable under different experimental conditions, and screening of suitable reference genes is a prerequisite in quantitative real-time polymerase chain reaction (qRT-PCR).
Wei Ye, Yongkai Tang, Fengyuan Yan, Shengyan Su, Jianlin Li, Xue Chen, Hui Li, Junjie Yu   +7 more
core   +1 more source