Results 21 to 30 of about 6,662,733 (309)

Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Trifolium repens L.) Involved in Five Abiotic Stresses

Plants, 2020
White clover (Trifolium repens L.) is a widely cultivated cool-season perennial forage legume in temperate grassland systems. Many studies have analyzed the gene expression in this grass species using quantitative real-time reverse transcription PCR (qRT-
Qi Pu, Zhou Li, Gang Nie, Jiqiong Zhou, Lin Liu, Yan Peng   +5 more
doaj   +1 more source

I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues in Arabidopsis thaliana

Biomolecules, 2023
Quantitative real-time polymerase chain reaction (qPCR) is a widely used method to analyse the gene expression pattern in the reproductive tissues along with detecting gene levels in mutant backgrounds.
Maria João Ferreira, Jessy Silva, Sara Cristina Pinto, Sílvia Coimbra   +3 more
doaj   +1 more source

Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]

, 2009
De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
De Gregoris, Tristano Bacchetti, Clare, Anthony S., Biffali, Elio, Elio Biffali, Thomas Bekel, Bekel, Thomas, Richard R Kirby, Borra, Marco, J Grant Burgess, Marco Borra, Burgess, J. Grant, Bacchetti De Gregoris, Tristano, Anthony S Clare, Kirby, Richard R., Tristano Bacchetti De Gregoris   +14 more
core   +1 more source

New Anti-inflammatory Cyclopeptides From a Sponge-Derived Fungus Aspergillus violaceofuscus

Frontiers in Chemistry, 2018
Three new cyclic peptides including a cyclic tetrapeptide (1), an aspochracin-type cyclic tripeptide sclerotiotide L (2) and a diketopiperazine dimer (3), have been isolated from the ethyl acetate extract of a marine sponge-derived fungus Aspergillus ...
Jingtang Liu, Binbin Gu, Lianjuan Yang, Fan Yang, Houwen Lin   +4 more
doaj   +1 more source

With Reference to Reference Genes: A Systematic Review of Endogenous Controls in Gene Expression Studies

PLOS ONE, 2015
The choice of reference genes that are stably expressed amongst treatment groups is a crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have specified that a minimum of two validated reference genes should be used for normalisation.
Joanne R Chapman, Jonas Waldenström
openaire   +4 more sources

Validation of Reference Genes in a Population of Blueberry (Vaccinium corymbosum) Plants Regenerated in Colchicine

Plants, 2022
For the first time we report the validation of reference genes in plants from a population of blueberry (Vaccinium corymbosum) clones cultured in vitro on a colchicine-supplemented medium.
Francisca Valenzuela, Vivían D’Afonseca, Ricardo Hernández, Aleydis Gómez, Ariel D. Arencibia   +4 more
doaj   +1 more source

miR-103a-3p and miR-22-5p Are Reliable Reference Genes in Extracellular Vesicles From Cartilage, Adipose Tissue, and Bone Marrow Cells

Frontiers in Bioengineering and Biotechnology, 2021
Cartilage cells (CCs), adipose tissue (ASC)- and bone marrow (BMSC)-derived mesenchymal stromal cells (MSCs) have been shown as promising candidates for the treatment of osteoarthritis (OA).
Enrico Ragni, Alessandra Colombini, Paola De Luca, Francesca Libonati, Marco Viganò, Carlotta Perucca Orfei, Luigi Zagra, Laura de Girolamo   +7 more
doaj   +1 more source

New methods to analyse microarray data that partially lack a reference signal. [PDF]

, 2009
BACKGROUND: Microarray-based Comparative Genomic Hybridisation (CGH) has been used to assess genetic variability between bacterial strains. Crucial for interpretation of microarray data is the availability of a reference to compare signal intensities to ...
Lindsay Jodi A, Fluit, AC, Rob JL Willems, Bonten, MJ, Willems Rob JL, Bonten Marc JM, Ad C Fluit, Carpaij, N, Lindsay, JA, Willems, RJ, Marc JM Bonten, Fluit Ad C, Jodi A Lindsay, Neeltje Carpaij, Carpaij Neeltje   +14 more
core   +1 more source

Selection and Validation of Reference Genes for Gene Expression Studies Using Quantitative Real-Time PCR in Prunus Necrotic Ringspot Virus-Infected Cucumis sativus

Viruses, 2022
Several members of the genus Ilarvirus infect fruit trees and are distributed worldwide. Prunus necrotic ringspot virus (PNRSV) is one of the most prevalent viruses, causing significant losses.
Zhenfei Dong, Binhui Zhan, Shifang Li
doaj   +1 more source

Selection of reference genes for gene expression studies in rats

Journal of Biotechnology, 2011
Selection of the most stable reference gene is critical for a reliable interpretation of gene expression data using RT-PCR. In order so, 17 commonly used genes were analyzed in Wistar rat duodenum, jejunum, ileum and liver following a fat gavage and at two time periods.
Martínez-Beamonte, R., Navarro, M. A., Larraga, A., Strunk, M., Barranquero, C., Acín, S., Guzman, M. A., Iñigo, P., Osada, J.   +8 more
openaire   +3 more sources