Results 41 to 50 of about 1,765,900 (302)

Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Normalization in Staminate and Perfect Flowers of Andromonoecious Taihangia rupestris

open access: yesFrontiers in Plant Science, 2017
Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is the most commonly used and powerful method for gene expression analysis due to its high sensitivity, specificity, and high throughput, and the accuracy of this approach ...
Weiguo Li   +6 more
doaj   +1 more source

Edinger-Westphal Nucleus [PDF]

open access: yes, 2008
This report contains a summary of expression patterns for genes that are enriched in the Edinger-Westphal nucleus (EW) of the midbrain. All data are derived from the Allen Brain Atlas (ABA) in situ hybridization mouse project.
Allen Institute for Brain Science   +3 more
core   +2 more sources

Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples

open access: yesFrontiers in Genetics, 2019
Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC.
Hui Wang   +13 more
doaj   +1 more source

Assessment of the specificity and stability of Micro-RNAs as a forensic gene marker

open access: yesBiomedical and Biotechnology Research Journal, 2023
Background: Forensic investigations depend on bodily fluid analysis to identify the perpetrators. Identifying perpetrators requires knowledge about suspects' body fluids.
Yasir Haider Al-Mawlah   +7 more
doaj   +1 more source

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia [PDF]

open access: yes, 2016
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug ...
Brian Dean   +2 more
core   +4 more sources

Selection of reference genes for gene expression studies in rats

open access: yesJournal of Biotechnology, 2011
Selection of the most stable reference gene is critical for a reliable interpretation of gene expression data using RT-PCR. In order so, 17 commonly used genes were analyzed in Wistar rat duodenum, jejunum, ileum and liver following a fat gavage and at two time periods.
Martínez-Beamonte, R.   +8 more
openaire   +3 more sources

Reference Genes for the Normalization of Gene Expression in Eucalyptus Species [PDF]

open access: yesPlant and Cell Physiology, 2011
Gene expression analysis is increasingly important in biological research, with reverse transcription-quantitative PCR (RT-qPCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Considering the increased sensitivity, reproducibility and large dynamic range of this method, the requirements for proper
Luisa Abruzzi, de Oliveira   +6 more
openaire   +2 more sources

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

open access: yesCumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş   +2 more
doaj   +1 more source

Optimal Reference Genes for Gene Expression Normalization in Trichomonas vaginalis

open access: yesPLOS ONE, 2015
Trichomonas vaginalis is the etiologic agent of trichomonosis, the most common non-viral sexually transmitted disease worldwide. This infection is associated with several health consequences, including cervical and prostate cancers and HIV acquisition.
Odelta dos Santos   +4 more
openaire   +4 more sources

Screening and Validation of Reference Genes for RT-qPCR Under Different Honey Bee Viral Infections and dsRNA Treatment

open access: yesFrontiers in Microbiology, 2020
Honey bee viruses are one of the most important pathogens that have contributed to the decrease in honey bee colony health. To analyze the infection dynamics of honey bee viruses, quantification of viral gene expression by RT-qPCR is necessary.
Yanchun Deng   +11 more
doaj   +1 more source

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