Results 61 to 70 of about 1,765,900 (302)

Validation of reference genes in human chordoma

Surgical Neurology International, 2017
Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference ...
Santegoeds, R G C, Yakkioui, Y, Jahanshahi, A, Hoogland, G, Temel, Y, van Overbeeke, J J   +5 more
openaire   +3 more sources

Sickle Cell Disease Is an Inherent Risk for Asthma in a Sibling Comparison Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Introduction Sickle cell disease (SCD) and asthma share a complex relationship. Although estimates vary, asthma prevalence in children with SCD is believed to be comparable to or higher than the general population. Determining whether SCD confers an increased risk for asthma remains challenging due to overlapping symptoms and the ...
Suhei C. Zuleta De Bernardis, Okeleji Olayinka, Mariam Z. Quraishi, Lakshmi V. Srivaths, Deborah L. Brown, Neethu M. Menon, Ricardo A. Mosquera, Ashwin P. Patel, James M. Stark, Aravind Yadav   +9 more
wiley   +1 more source

Establishing Reference Genes for Accurate Gene Expression Profiling in Toxigenic Bacillus cereus

Toxins
Bacillus cereus is a Gram-positive pathogen associated with foodborne illnesses and severe non-gastrointestinal infections. Robust tools for accurate gene transcription analysis are essential for studying toxin gene expression dynamics and deciphering ...
Tanja V. Edelbacher, Astrid Laimer-Digruber, Michael W. Pfaffl, Monika Ehling-Schulz   +3 more
doaj   +1 more source

Assessment of Suitable Reference Genes for RT-qPCR Normalization with Developmental Samples in Pacific Abalone Haliotis discus hannai

Journal of Animal Reproduction and Biotechnology, 2019
Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different ...
Sang Yoon Lee, Choul-Ji Park, Yoon Kwon Nam   +2 more
doaj   +1 more source

Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks

Frontiers in Veterinary Science, 2023
Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui, Fei Shui, Guiru Qiu, Shenqiang Pan, Xin Wang, Xin Wang, Fumin Jia, Fumin Jia, Tingting Jiang, Yongsheng Li, Zhaoyu Geng, Zhaoyu Geng, Sihua Jin, Sihua Jin   +13 more
doaj   +1 more source

How to perform RT-qPCR accurately in plant species?: a case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population [PDF]

, 2013
Background: Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids).
De Keyser, Ellen, De Riek, Jan, Desmet, Laurence, Van Bockstaele, Erik   +3 more
core   +2 more sources

Reference Genes for Quantitative Gene Expression Studies in Multiple Avian Species

PLoS ONE, 2014
Quantitative real-time PCR (qPCR) rapidly and reliably quantifies gene expression levels across different experimental conditions. Selection of suitable reference genes is essential for meaningful normalization and thus correct interpretation of data.
Olias, Philipp, Adam, Iris, Meyer, Anne, Scharff, Constance, Gruber, Achim Dieter   +4 more
openaire   +6 more sources

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis [PDF]

Frontiers in Genetics, 2020
Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis. Therefore, it is the primary task to select and validate the appropriate ginger reference gene to normalize the expression of target genes. In this study, 14 candidate reference genes were
Yao Lv, Yao Lv, Yao Lv, Yao Lv, Yanyan Li, Xiaohui Liu, Kun Xu, Kun Xu, Kun Xu, Kun Xu   +9 more
openaire   +3 more sources

Psychosocial Outcomes in Patients With Endocrine Tumor Syndromes: A Systematic Review

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Introduction The combination of disease manifestations, the familial burden, and varying penetrance of endocrine tumor syndromes (ETSs) is unique. This review aimed to portray and summarize available data on psychosocial outcomes in patients with ETSs and explore gaps and opportunities for future research and care.
Daniël Zwerus, Floortje Strobbe, Annemarie A. Verrijn Stuart, Hanneke M. van Santen, Gerlof D. Valk, Sasja A. Schepers, Rachel S. van Leeuwaarde   +6 more
wiley   +1 more source

Identification and Analysis of Reference and Tissue-Specific Genes in Bitter Gourd Based on Transcriptome Data

Horticulturae, 2023
Accurate and standardized quantification of reverse transcription PCR (qRT-PCR) results relies on the use of a dependable reference gene. The precise control of transgene expression in terms of both spatial and temporal aspects necessitates the ...
Yangyi Zheng, Yao Ma, Jianning Luo, Junxing Li, Xiaoming Zheng, Hao Gong, Liting Deng, Gangjun Zhao, Caixia Luo, Xiaoxi Liu, Haibin Wu   +10 more
doaj   +1 more source