Results 61 to 70 of about 1,760,212 (203)

The Douglas-Fir Genome Sequence Reveals Specialization of the Photosynthetic Apparatus in Pinaceae. [PDF]

, 2017
A reference genome sequence for Pseudotsuga menziesii var. menziesii (Mirb.) Franco (Coastal Douglas-fir) is reported, thus providing a reference sequence for a third genus of the family Pinaceae.
Cardeno, Charis, Casola, Claudio, Crepeau, Marc W, Cronn, Richard, Gonzalez-Ibeas, Daniel, Holt, Carson, Koralewski, Tomasz E, Langley, Charles H, McGuire, Patrick E, Neale, David B, Paul, Robin, Pertea, Geo M, Puiu, Daniela, Salzberg, Steven L, Sezen, U Uzay, Stevens, Kristian A, Wegrzyn, Jill L, Wheeler, Nicholas C, Yandell, Mark, Yorke, James A, Zaman, Sumaira, Zimin, Aleksey V   +21 more
core   +2 more sources

Validation of reference genes in human chordoma

Surgical Neurology International, 2017
Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference ...
Santegoeds, R G C, Yakkioui, Y, Jahanshahi, A, Hoogland, G, Temel, Y, van Overbeeke, J J   +5 more
openaire   +3 more sources

Establishing Reference Genes for Accurate Gene Expression Profiling in Toxigenic Bacillus cereus

Toxins
Bacillus cereus is a Gram-positive pathogen associated with foodborne illnesses and severe non-gastrointestinal infections. Robust tools for accurate gene transcription analysis are essential for studying toxin gene expression dynamics and deciphering ...
Tanja V. Edelbacher, Astrid Laimer-Digruber, Michael W. Pfaffl, Monika Ehling-Schulz   +3 more
doaj   +1 more source

Assessment of Suitable Reference Genes for RT-qPCR Normalization with Developmental Samples in Pacific Abalone Haliotis discus hannai

Journal of Animal Reproduction and Biotechnology, 2019
Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different ...
Sang Yoon Lee, Choul-Ji Park, Yoon Kwon Nam   +2 more
doaj   +1 more source

Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks

Frontiers in Veterinary Science, 2023
Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui, Fei Shui, Guiru Qiu, Shenqiang Pan, Xin Wang, Xin Wang, Fumin Jia, Fumin Jia, Tingting Jiang, Yongsheng Li, Zhaoyu Geng, Zhaoyu Geng, Sihua Jin, Sihua Jin   +13 more
doaj   +1 more source

Identification of Importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens [PDF]

, 2008
Background: The accurate normalization of differentially expressed genes in lung cancer is essential for the identification of novel therapeutic targets and biomarkers by real time RT-PCR and microarrays.
Nguewa, P.A. (Paul Alain), Agorreta, J. (Jackeline), Blanco, D. (D.), Lozano, M.D. (María Dolores), Gomez-Roman, J. (Javier), Sanchez, B.A. (Blas A.), Valles, I. (Iñaki), Pajares, M.J. (María José), Pio, R. (Rubén), Rodriguez, M.J. (María José), Montuenga-Badia, L.M. (Luis M.), Calvo-González, A. (Alfonso)   +11 more
core   +1 more source

Reference Genes for Quantitative Gene Expression Studies in Multiple Avian Species

PLoS ONE, 2014
Quantitative real-time PCR (qPCR) rapidly and reliably quantifies gene expression levels across different experimental conditions. Selection of suitable reference genes is essential for meaningful normalization and thus correct interpretation of data.
Olias, Philipp, Adam, Iris, Meyer, Anne, Scharff, Constance, Gruber, Achim Dieter   +4 more
openaire   +6 more sources

Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis [PDF]

Frontiers in Genetics, 2020
Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis. Therefore, it is the primary task to select and validate the appropriate ginger reference gene to normalize the expression of target genes. In this study, 14 candidate reference genes were
Yao Lv, Yao Lv, Yao Lv, Yao Lv, Yanyan Li, Xiaohui Liu, Kun Xu, Kun Xu, Kun Xu, Kun Xu   +9 more
openaire   +3 more sources

Identification and Analysis of Reference and Tissue-Specific Genes in Bitter Gourd Based on Transcriptome Data

Horticulturae, 2023
Accurate and standardized quantification of reverse transcription PCR (qRT-PCR) results relies on the use of a dependable reference gene. The precise control of transgene expression in terms of both spatial and temporal aspects necessitates the ...
Yangyi Zheng, Yao Ma, Jianning Luo, Junxing Li, Xiaoming Zheng, Hao Gong, Liting Deng, Gangjun Zhao, Caixia Luo, Xiaoxi Liu, Haibin Wu   +10 more
doaj   +1 more source

Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata) [PDF]

, 2018
Background Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN).
Egea Gutiérrez-Cortines, Marcos, Fernández, Juan A., Letourneux, Lisa, Martos Fuentes, Marina Marta, Ruiz Hernández, María Víctoria, Terry López, Marta Isabel, Weiss, Julia Rosl   +6 more
core   +4 more sources