Results 1 to 10 of about 1,185,109 (150)

New Approaches to Multi-Parametric HIV-1 Genetics Using Multiple Displacement Amplification: Determining the What, How, and Where of the HIV-1 Reservoir

Viruses, 2021
Development of potential HIV-1 curative interventions requires accurate characterization of the proviral reservoir, defined as host-integrated viral DNA genomes that drive rebound of viremia upon halting ART (antiretroviral therapy).
Sean C. Patro, Aurelie Niyongabo, Frank Maldarelli, Mary F. Kearney   +3 more
doaj   +1 more source

The Role of Capsid in HIV-1 Nuclear Entry

Viruses, 2021
HIV-1 can infect non-dividing cells. The nuclear envelope therefore represents a barrier that HIV-1 must traverse in order to gain access to the host cell chromatin for integration.
Anabel Guedán, Eve R. Caroe, Genevieve C. R. Barr, Kate N. Bishop   +3 more
doaj   +1 more source

Why the HIV Reservoir Never Runs Dry: Clonal Expansion and the Characteristics of HIV-Infected Cells Challenge Strategies to Cure and Control HIV Infection

Viruses, 2021
Antiretroviral therapy (ART) effectively reduces cycles of viral replication but does not target proviral populations in cells that persist for prolonged periods and that can undergo clonal expansion.
Chuen-Yen Lau, Matthew A. Adan, Frank Maldarelli   +2 more
doaj   +1 more source

Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CL^pro Inhibitors

Viruses, 2021
The 3C-like protease (3CLpro) of SARS-CoV-2 is considered an excellent target for COVID-19 antiviral drug development because it is essential for viral replication and has a cleavage specificity distinct from human proteases.
Jonathan M. O. Rawson, Alice Duchon, Olga A. Nikolaitchik, Vinay K. Pathak, Wei-Shau Hu   +4 more
doaj   +1 more source

Plasma Membrane Anchoring and Gag:Gag Multimerization on Viral RNA Are Critical Properties of HIV-1 Gag Required To Mediate Efficient Genome Packaging

mBio, 2021
Human immunodeficiency virus type 1 (HIV-1) Gag selects and packages the HIV RNA genome during virus assembly. However, HIV-1 RNA constitutes only a small fraction of the cellular RNA.
Alice Duchon, Steven Santos, Jianbo Chen, Matthew Brown, Olga A. Nikolaitchik, Sheldon Tai, Jeffrey A. Chao, Eric O. Freed, Vinay K. Pathak, Wei-Shau Hu   +9 more
doaj   +1 more source

MTBP phosphorylation controls DNA replication origin firing

Scientific Reports, 2021
Faithful genome duplication requires regulation of origin firing to determine loci, timing and efficiency of replisome generation. Established kinase targets for eukaryotic origin firing regulation are the Mcm2-7 helicase, Sld3/Treslin/TICRR and Sld2 ...
Pedro Ferreira, Verena Höfer, Nora Kronshage, Anika Marko, Karl-Uwe Reusswig, Bilal Tetik, Christoph Dießel, Kerstin Köhler, Nikolai Tschernoster, Janine Altmüller, Nina Schulze, Boris Pfander, Dominik Boos   +12 more
doaj   +1 more source

The mechanism of replication stalling and recovery within repetitive DNA

Nature Communications, 2022
DNA replication of repetitive sequences was recreated in a test tube using purified components. DNA alone was sufficient to induce stalling. Both stalling and recovery were dictated by the capacity of DNA to fold into unusual secondary structures.
Corella S. Casas-Delucchi, Manuel Daza-Martin, Sophie L. Williams, Gideon Coster   +3 more
doaj   +1 more source

Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication

mBio, 2022
Frequent recombination is a hallmark of retrovirus replication. In rare cases, recombination occurs between distantly related retroviruses, generating novel viruses that may significantly impact viral evolution and public health.
Jonathan M. O. Rawson, Olga A. Nikolaitchik, Jennifer A. Yoo, Xayathed Somoulay, Matthew A. Brown, Franck S. Mbuntcha Bogni, Vinay K. Pathak, Ferri Soheilian, Ryan L. Slack, Stefan G. Sarafianos, Wei-Shau Hu   +10 more
doaj   +1 more source

Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation

eLife, 2015
We have reconstituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides. This study identifies a process unprecedented in bacterial replisomes.
Roxana E Georgescu, Grant D Schauer, Nina Y Yao, Lance D Langston, Olga Yurieva, Dan Zhang, Jeff Finkelstein, Mike E O'Donnell   +7 more
doaj   +1 more source

Restriction of Influenza A Virus by SERINC5

mBio, 2022
Serine incorporator 5 (Ser5), a transmembrane protein, has recently been identified as a host antiviral factor against human immunodeficiency virus (HIV)-1 and gammaretroviruses like murine leukemia viruses (MLVs). It is counteracted by HIV-1 Nef and MLV
Kin Kui Lai, James B. Munro, Guoli Shi, Saliha Majdoul, Alex A. Compton, Alan Rein   +5 more
doaj   +1 more source