Results 61 to 70 of about 2,229,811 (302)

Cdt1 stabilizes an open MCM ring for helicase loading

Nature Communications, 2017
The loading and activation of the Mcm2-7 replicative helicase couples cell cycle progression to DNA replication. Here the authors use X-ray crystallography and single-particle electron microscopy to demonstrate how Ctd1 functions to promote MCM loading ...
Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E. Pye, Ludovic Renault, Max E. Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F. X. Diffley   +9 more
doaj   +1 more source

Evolutionary rates and gene dispensability associate with replication timing in the Archaeon Sulfolobus islandicus [PDF]

, 2010
In bacterial chromosomes, the position of a gene relative to the single origin of replication generally reflects its replication timing, how often it is expressed, and consequently, its rate of evolution.
Cooper, Vaughn S., Flynn, Kenneth M., Hatcher, Philip J., Vohr, Samuel H.   +3 more
core   +2 more sources

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Development of a Complementation Assay to Monitor Pan-Coronavirus 3C-like Protease Activity

Viruses
Coronaviruses pose a global pandemic threat, making development of a pan-coronavirus inhibitor crucial for preparedness and containment in the event of a new coronavirus outbreak.
Akhil Chameettachal, Alice Duchon, Matthew A. Brown, Jonathan M. O. Rawson, Vinay K. Pathak, Wei-Shau Hu   +5 more
doaj   +1 more source

The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]

, 2012
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Anton Gartner, Ashley Craig, Baldinger, Blow, Blow, Brauchle, Brenner, Cheeseman, Chen, Clarey, Clarey, DePamphilis, Drury, Edgar, Encalada, Evrin, Galy, Gambus, Gillespie, Hadwiger, Harvey, Ilves, J. Julian Blow, Kamath, Kim, Kisielewska, Korzelius, Kuipers, Li, Li, Li, Liu, Matthieu Querenet, McNairn, McNally, Moyer, Oehlmann, Praitis, Prokhorova, Quinn, Randell, Remi Sonneville, Remus, Rothbauer, Rowles, Shelton, Siddiqui, Speck, Sönnichsen, Tada, Timmons, Waga, Wallenfang, Wohlschlegel, Yanagi, Yang, Zhong   +56 more
core   +4 more sources

Replication challenges [PDF]

Science, 2019
The ability to test validity by replicating experiments and comparing results is a cornerstone of science. The events that followed publication of “Sustained virologic control in SIV + macaques after antiretroviral and α 4 β 7 antibody therapy” by Byrareddy
openaire   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Distinct Contributions of Different Domains within the HIV-1 Gag Polyprotein to Specific and Nonspecific Interactions with RNA

Viruses, 2020
Viral genomic RNA is packaged into virions with high specificity and selectivity. However, in vitro the Gag specificity towards viral RNA is obscured when measured in buffers containing physiological salt. Interestingly, when the binding is challenged by
Tomas Kroupa, Siddhartha A. K. Datta, Alan Rein   +2 more
doaj   +1 more source

Replicating nucleosomes [PDF]

Science Advances, 2015
Reestablishment of the nucleosome landscape behind the replication fork is fundamental for epigenetic memory during development.
Ramachandran, Srinivas, Henikoff, Steven
openaire   +2 more sources