Results 81 to 90 of about 1,350,402 (318)

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of trypanosoma brucei [PDF]

, 2012
Identification of replication initiation sites, termed origins, is a crucial step in understanding genome transmission in any organism. Transcription of the Trypanosoma brucei genome is highly unusual, with each chromosome comprising a few discrete ...
Dickens, N.J., Dickens, N, Kelly, S, Marcello, Lucio, Swiderski, M, Stephen D. Bell, Farr, Helen, Farr, H., Swiderski, M., Vaughan, D., Diane Vaughan, Bell, Stephen D, Nicholas Dickens, Marcello, L., Steven Kelly, Tiengwe, C, Dickens, Nicholas, Marcello, L, Bell, SD, Bell, Stephen D., Vaughan, D, McCulloch, R, Kelly, S., Swiderski, Michal, Keith Gull, Tiengwe, Calvin, Barry, J.D., McCulloch, R., Barry, J David, Barry, JD, Farr, H, Gull, K, Lucio Marcello, Richard McCulloch, J. David Barry, Bell, S.D., Calvin Tiengwe, McCulloch, Richard, Kelly, Steven, Helen Farr, Tiengwe, C., Gull, Keith, Barry, J. David, Vaughan, Diane, Gull, K., Michal Swiderski   +45 more
core   +1 more source

An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

eLife, 2020
Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein ...
Julia Bittmann, Rokas Grigaitis, Lorenzo Galanti, Silas Amarell, Florian Wilfling, Joao Matos, Boris Pfander   +6 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

PARK(ing) time–How park deficiency affects the biological clock in a Drosophila model of Parkinson's disease

FEBS Letters, EarlyView.
Drosophila park mutants serve as a model for Parkinson's disease. We used this strain to investigate the connection between oxidative stress and the circadian clock mechanism. We showed that increased oxidative stress affects the physiology of pacemaker cells, disrupting their daily structural plasticity. Lack of rhythmic signaling from pacemaker cells
Kamila Zientara, Kinga Skoczek, Elzbieta Pyza, Milena Damulewicz   +3 more
wiley   +1 more source

GEMC1, a novel factor required for chromosomal DNA replication

, 2009
In eukaryotic cells DNA replication begins from multiple origins. During the process of initiation, the DNA replication fork is established at each origin. In lower eukaryotes many factors required for chromosomal DNA replication have been identified.
Balestrini, A.
core  

RAD51 restricts DNA over-replication from re-activated origins

The EMBO Journal
Eukaryotic cells rely on several mechanisms to ensure that the genome is duplicated precisely once in each cell division cycle, preventing DNA over-replication and genomic instability.
Sergio Muñoz, Elena Blanco-Romero, Daniel González-Acosta, Sara Rodriguez-Acebes, Diego Megías, Massimo Lopes, Juan Méndez   +6 more
doaj   +1 more source

Development of Lentiviral Vectors for HIV-1 Gene Therapy with Vif-Resistant APOBEC3G

Molecular Therapy: Nucleic Acids, 2019
Strategies to control HIV-1 replication without antiviral therapy are needed to achieve a functional cure. To exploit the innate antiviral function of restriction factor cytidine deaminase APOBEC3G (A3G), we developed self-activating lentiviral vectors ...
Krista A. Delviks-Frankenberry, Daniel Ackerman, Nina D. Timberlake, Maria Hamscher, Olga A. Nikolaitchik, Wei-Shau Hu, Bruce E. Torbett, Vinay K. Pathak   +7 more
doaj   +1 more source