Results 1 to 10 of about 7,567,038 (395)
Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo [PDF]
Cells, 2023 In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced.
Monford Paul Abishek N +3 more
doaj +4 more sources
Magnetic Resonance Reporter Gene Imaging [PDF]
Theranostics, 2012 Molecular imaging has undergone an explosive advancement in recent years, due to the tremendous research efforts made to understand and visualize biological processes.
Sheen-Woo Lee, Sang-Hoon Lee, Sandip Biswal
doaj +4 more sources
Reporter gene assay for membrane fusion of extracellular vesicles. [PDF]
J Extracell Vesicles, 2021 Extracellular vesicles (EVs) secreted by living cells are expected to deliver biological cargo molecules, including RNA and proteins, to the cytoplasm of recipient cells.
Somiya M, Kuroda S.
europepmc +2 more sources
Enrichment of transgene integrations by transient CRISPR activation of a silent reporter gene [PDF]
Molecular Therapy: Methods & Clinical Development, 2023 CRISPR-Cas-mediated site-specific integration of transgenes by homology-directed repair (HDR) is challenging, especially in primary cells, where inferior editing efficiency may impede the development of gene- and cellular therapies.
Nanna S. Mikkelsen +4 more
doaj +2 more sources
Tyrosinase-Based Reporter Gene for Photoacoustic Imaging of MicroRNA-9 Regulated by DNA Methylation in Living Subjects [PDF]
Molecular Therapy: Nucleic Acids, 2018 MicroRNAs (miRNAs) are a class of negative regulators of gene expression and play critical roles in various biological processes. Conventional approaches for detecting miRNAs, such as northern blotting, microarray, and real-time PCR, usually require the ...
Haifeng Zheng +4 more
doaj +2 more sources
The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis [PDF]
Journal of Experimental Life Science, 2015 Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer.
Septi Anitasari +2 more
doaj +2 more sources
Science Advances, 2021 A CRISPR-based system enables safer multimodal in vivo reporter gene cell tracking. Imaging reporter genes provides longitudinal information on the biodistribution, growth, and survival of engineered cells in vivo.
J. Kelly +7 more
semanticscholar +1 more source
Molecular Therapy: Oncolytics, 2021 Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals.
Maxim A. Moroz +10 more
doaj +1 more source
Frontiers in Oncology, 2021 BackgroundMicroRNAs (miRs) have been shown to be closely associated with the occurrence and development of tumors and to have potential as diagnostic and therapeutic targets.
Guangcheng Bao +31 more
doaj +1 more source
Biosensors, 2023 Reporter genes are important tools in many biological disciplines. The discovery of novel reporter genes is relatively rare. However, known reporter genes are constantly applied to novel applications.
Céline S. M. Richard +4 more
doaj +1 more source