Results 41 to 50 of about 9,624 (189)

Amplex Red cellular uptake produces radical intermediates by myeloperoxidase and mediates oxidative stress

Redox Biochemistry and Chemistry
Amplex Red (AR) is commonly used to detect extracellular hydrogen peroxide (H2O2) and is considered a cell-impermeable compound. However, it would appear capable of entering cells based on its phenoxazine substructure and the report of its mitochondrial ...
Huan Liu, Lusine Tonoyan, Béla Reiz, Arno G. Siraki   +3 more
doaj   +1 more source

A cost-effective, analytical method for measuring metabolic load of mitochondria

Metabolism Open, 2019
Analysis of cellular energetics is central to understanding metabolic diseases including diabetes and cancer. The two most commonly used methods to monitor cellular respiration are the Seahorse-XF system, and Glo™ assays, which are considered “gold ...
James F.E. Grey, Amelia R. Townley, Nicola M. Everitt, Alistair Campbell-Ritchie, Sally P. Wheatley   +4 more
doaj   +1 more source

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek, Jean‐Marc Daran, Marieke E. Klijn, Marcel A. Vieira‐Lara   +3 more
wiley   +1 more source

Cellular Viability of Partial Heart Transplant Grafts in Cold Storage

Frontiers in Surgery, 2021
Congenital heart defects are the most common types of birth defects in humans. Children with congenital heart defects frequently require heart valve replacement with an implant. Unfortunately, conventional heart valve implants do not grow.
Jennie H. Kwon, Morgan Ashley Hill, Brielle Gerry, Jordan Morningstar, Minoo N. Kavarana, Satish N. Nadig, Taufiek Konrad Rajab   +6 more
doaj   +1 more source

Shedding Light on Synthetic Autocatalysis: From Conventional Closed‐Shell Chemistries to Overlooked Open‐Shell Occurrences

Chemistry – A European Journal, EarlyView.
Why add another catalyst when the product itself holds the power to catalyze its own formation? Autocatalysis in synthetic chemistry enhances reaction efficiency and uncovers novel catalytic behavior across both closed‐shell and open‐shell systems, expanding reactivity and enabling innovative design strategies.
Jaspreet Kaur, Joshua P. Barham
wiley   +1 more source

Myocardial inflammation is associated with impaired mitochondrial oxidative capacity in ischaemic cardiomyopathy

ESC Heart Failure, Volume 12, Issue 2, Page 1246-1255, April 2025.
Abstract Aims Myocardial inflammation and impaired mitochondrial oxidative capacity are hallmarks of heart failure (HF) pathophysiology. The extent of myocardial inflammation in patients suffering from ischaemic cardiomyopathy (ICM) or dilated cardiomyopathy (DCM) and its association with mitochondrial energy metabolism are unknown.
Julius Borger, Elric Zweck, Constanze Moos, Patrick Horn, Fabian Voß, Heinz‐Peter Schultheiss, Jacob Eifer Møller, Udo Boeken, Hug Aubin, Artur Lichtenberg, Malte Kelm, Michael Roden, Amin Polzin, Ralf Westenfeld, Julia Szendroedi, Daniel Scheiber   +15 more
wiley   +1 more source

Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red

Bio-Protocol, 2016
This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit.
Tzvetina Brumbarova, Cham Le, Petra Bauer   +2 more
doaj   +1 more source

Body‐integrated photonic biosensors: Illuminating the path to active healthcare

FlexMat, EarlyView.
Body‐integrated photonic biosensors are promising tools for active healthcare. These optical devices can be worn, implanted, or swallowed to monitor health signals continuously. This review introduces key sensing modalities, including fluorescence, colorimetry, SPR, LSPR, SERS, and light‐modulating materials.
Jiayue Gu, Xingming Wen, Qihang Yan, Yining Zhao, Linghao Yuan, Siyuan Chen, Yue Ma, Zhengyue Fang, Dong Lei, Yuxin Liu, Ze Xiong   +10 more
wiley   +1 more source

Electrochemical modification and analytical exploration of resazurin as a redox-active probe for electrochemical biosensors

Electrochemistry Communications
An electrochemical potential-assisted functionalization strategy is used to immobilize resazurin (AZ) on multiwalled carbon nanotube surfaces in a physiological buffer leading to the formation of a resorufin/dihydro resorufin (RR/DRR) redox couple.
Balamurugan Thangavel, Won Han, Joong Ho Shin   +2 more
doaj   +1 more source

Glycoside Hydrolase Activities in Cell Walls of Sclerenchyma Cells in the Inflorescence Stems of Arabidopsis thaliana Visualized in Situ

Plants, 2014
Techniques for in situ localization of gene products provide indispensable information for understanding biological function. In the case of enzymes, biological function is directly related to activity, and therefore, knowledge of activity patterns is ...
Alicja Banasiak, Farid M. Ibatullin, Harry Brumer, Ewa J. Mellerowicz   +3 more
doaj   +1 more source