Results 61 to 70 of about 9,624 (189)

Osmotic Remodeling of Extracellular Vesicles for Precision Nanomedicine

Small, EarlyView.
Hypotonic lysis in bidistilled water is used to remodel B lymphocyte–derived extracellular vesicles into cargo‐depleted, structurally preserved nanovesicles. The process reduces intraluminal proteins and nucleic acids, reshapes membrane composition, and enhances tumor‐cell uptake in vitro, while allowing proof‐of‐concept reloading with exogenous agents,
Francesca Susa, Lorenzo Arnaboldi, Veronica De Giorgis, Marco Ghirimoldi, Elettra Barberis, Marco Brucale, Francesco Valle, Mario Malerba, Marta Vallino, Angelo Musicò, Roberto Frigerio, Alessandro Gori, Vera Mugoni, Claudia Voena, Roberto Pisano, Fabrizio Pirri, Silvia Arpicco, Marcello Manfredi, Tania Limongi   +18 more
wiley   +1 more source

In vitro Screening of Antileishmanial Activity of Natural Product Compounds: Determination of IC50, CC50 and SI Values

Bio-Protocol, 2019
Neglected tropical diseases gain the scientific interest of numerous research programs in an attempt to achieve their effective control or elimination. In this attempt, more cutting-edge public health policies and research are needed for the discovery of
Olga Koutsoni, Kalliopi Karampetsou, Eleni Dotsika   +2 more
doaj   +1 more source

Ultrasensitive Detection of Neurofilament Light in Plasma Using F(Ab’)2‐Modified Graphene Field‐Effect Biosensor

Small, EarlyView.
An antibody fragment (F(ab')2)‐modified graphene field‐effect transistor biosensor is developed for the ultrasensitive detection of neurofilament light chain in clinical plasma. By reducing the Debye screening effect, the platform achieves a 114% sensitivity increase and a fivefold lower detection limit compared to whole‐antibody approaches ...
Selvinaz Burcu Kizilates, Rica Asrosa, Lenart Senicar, Anuja Sharma, Seda Gungordu Er, Nisha Naeem, Ahmad Nizamuddin bin Muhammad Mustafa, Yang Wu, Neil Graham, Amanda Heslegrave, Mohan Edirisinghe, Antonio Lombardo, Elias Torres, Henrik Zetterberg, Sami Ramadan, David J. Sharp, Bing Li   +16 more
wiley   +1 more source

Targeted, receptor‐mediated delivery of a masked d‐amino acid cell‐penetrating peptide for cell‐specific phototoxicity

The FEBS Journal, EarlyView.
A targeted phototoxic peptide selectively accumulates in cells expressing chemokine‐like receptor 1 (CMKLR1). Phototoxicity of the native peptide is low, but cleavage by lysosomal protease cathepsin B releases a 6‐tetramethylrhodamine (TMR) labeled nona‐ᴅ‐arginine which mediates lysosomal rupture and cell death upon irradiation.
Moritz List, Eva‐Maria Jülke, Annette G. Beck‐Sickinger   +2 more
wiley   +1 more source

Physiological media and oxygen levels modulate cancer hallmarks in cultured breast cancer cells

The FEBS Journal, EarlyView.
MCF7 breast cancer cells were cultured in standard conditions (18% O2 and DMEM) and physiological conditions (5% O2 and Plasmax, a medium modeled after the human plasma metabolome). A combined transcriptomic and proteomic approach was used to identify gene expression signatures at the mRNA and protein levels upstream of functional enrichment analyses ...
Jacob E. Wiebe, Ricardo Alva, Fereshteh Moradi, Sarah Alderman, Ping Liang, Jeffrey A. Stuart   +5 more
wiley   +1 more source

Enzymatic measurement of phosphatidic acid in cultured cells

Journal of Lipid Research, 2009
In this work, we developed a novel enzymatic method for measuring phosphatidic acid (PA) in cultured cells. The enzymatic reaction sequence of the method involves hydrolysis of PA to produce glycerol-3-phosphate (G3P), which is then oxidized by G3P ...
Shin-ya Morita, Kazumitsu Ueda, Shuji Kitagawa   +2 more
doaj   +1 more source

Neurotrophic extracellular matrix proteins promote neuronal and iPSC astrocyte progenitor cell‐ and nano‐scale process extension for neural repair applications

Journal of Anatomy, Volume 246, Issue 4, Page 585-601, April 2025.
The extracellular matrix plays a critical role in modulating cell behaviour in the central nervous system influencing neural cell morphology and growth. However, a better understanding of the impact of individual matrix proteins on both neurons and astrocytes is critical for advancing the development of matrix‐based neural repair strategies.
Cian O'Connor, Rena E. Mullally, Sarah F. McComish, Julia O'Sullivan, Ian Woods, Ingmar Schoen, Massimiliano Garre, Maeve A. Caldwell, Adrian Dervan, Fergal J. O'Brien   +9 more
wiley   +1 more source

Dyes in Diagnostics

CHIMIA, 1991
Dyes and dye precursors are often essential components in reagents for medical diagnostics. The type and the attributes of suitable dyes depend on the basis of the technology used for the analysis of the diagnostic parameter to be determined. To
Rupert Herrmann
doaj   +2 more sources

Riboflavin Salvage Supports Glycolysis in Borrelia burgdorferi Through Flavin‐Dependent NAD+ Regeneration

Molecular Microbiology, EarlyView.
The Lyme disease agent Borrelia burgdorferi has limited metabolic capabilities and depends on salvage of riboflavin via the BB0316‐BB0319 transport system for generation of critical flavin cofactors FMN and FAD. Riboflavin is important for NAD+ regeneration to fuel glycolysis.
Anna M. Schulz, Grace E. Easterling, Aaditya Balasubramaniyan, Mollie W. Jewett   +3 more
wiley   +1 more source

Mitochondrial physiology in cardiac muscle of deer mice native to high altitude

The Journal of Physiology, EarlyView.
Abstract figure legend High‐altitude deer mice exhibited evolved changes in mitochondrial energy metabolism and reactive oxygen species (ROS) management that may support cardiac performance under cold hypoxic conditions. High‐altitude mice had increased activity of lactate dehydrogenase (LDH) in the heart, probably enhancing the capacity for lactate ...
Ranim Saleem, Samuel G. Robichaud, Simon G. Lamarre, Graham R. Scott   +3 more
wiley   +1 more source