Results 151 to 160 of about 8,183 (298)

In Vivo Cytoskeletal AMPA Receptor Transport Imaging in C. elegans

open access: yesCytoskeleton, EarlyView.
ABSTRACT Long‐distance intracellular transport of ionotropic glutamate receptors (iGluRs) is essential for proper excitatory synaptic function underlying learning and memory. Many neuropsychiatric and neurodegenerative conditions have abnormal iGluR transport and trafficking, leading to an intense interest in the mechanisms and factors regulating these
Michaelis A. K., Hoerndli F. J.
wiley   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

open access: yesCytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

open access: yesCytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham   +3 more
wiley   +1 more source

Labeling Strategies for Monitoring Cytoskeleton Dynamics in Cultured Epithelial Cells

open access: yesCytoskeleton, EarlyView.
ABSTRACT Collective cell migration relies on coordinated cytoskeletal remodeling, yet the impact of live‐cell actin filament probes on these dynamics remains poorly characterized. Here, we systematically compared the performance and cellular effects of fluorogenic jasplakinolide‐based probes, SiR‐actin and SiR‐XActin, with the genetically encoded ...
Victoria Levario‐Diaz   +6 more
wiley   +1 more source

Hyperactive KIF5A in Neurodegeneration

open access: yesCytoskeleton, EarlyView.
ABSTRACT The highly polarised morphology of neurons and the sheer length of their axons make transport of cargoes throughout the cell a formidable task. Decades of evidence obtained from genetic studies on patients and animal models highlight deficits in axonal transport as a recurrent cause, or early contributing factor, in a plethora of ...
David Villarroel‐Campos   +1 more
wiley   +1 more source

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