Reverse Genetics System for Shuni Virus, an Emerging Orthobunyavirus with Zoonotic Potential [PDF]
, 2020 Judith Oymans +7 more
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Survivin and Aurora Kinase A control cell fate decisions during mitosis
Molecular Oncology, EarlyView.Aurora A interacts with survivin during mitosis and regulates its centromeric role. Loss of Aurora A activity mislocalises survivin, the CPC and BubR1, leading to disruption of the spindle checkpoint and triggering premature mitotic exit, which we refer to as ‘mitotic slippage’.
Hana Abdelkabir +2 more
wiley +1 more source
THE USE OF REVERSE GENETICS APPROACH IN PLANT GENOMICS [PDF]
Poljoprivreda, 2012 Mutations are origin of novel variabilities. With developing new methods there are new possibilities for plant breeding for desirable characteristics. The aim of this paper is to introduce a method of reverse genetics, particularly TILLING method, and ...
Ruđer Šimek, Dario Novoselović
doaj
Phenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses [PDF]
, 2001 Vira Bitko, Sailen Barik
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Molecular Oncology, EarlyView.CDK11 inhibition stabilises the tumour suppressor p53 and triggers the production of an alternative p21WAF1 splice variant p21L, through the inactivation of the spliceosomal protein SF3B1. Unlike the canonical p21WAF1 protein, p21L is localised in the cytoplasm and has reduced cell cycle‐blocking activity.
Radovan Krejcir +12 more
wiley +1 more source
Molecular Oncology, EarlyView.A mouse model for vascular normalization and a human breast cancer cohort were studied to understand the relationship between vascular leakage and tumor immune suppression. For this, endothelial and immune cell RNAseq, staining for vascular function, and immune cell profiling were employed.
Liqun He +8 more
wiley +1 more source
Introducing undergraduate students to reverse genetics in the laboratory: a case study of a project-oriented learning (POL) system in Taishan Medical University, China [PDF]
, 2020 Lijuan Yu, Henglong Zhang, Jing Zhai
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Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA
Molecular Oncology, EarlyView.Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson +2 more
wiley +1 more source
Identification of Host Genes Involved in Geminivirus Infection Using a Reverse Genetics Approach
, 2011 Rosa Lozano‐Durán +3 more
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