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Reverse Genetics for Mammalian Orthoreovirus

2017
Reverse genetics allows introduction of specific alterations into a viral genome. Studies performed with mutant viruses generated using reverse genetics approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors.
Johnasha D, Stuart   +2 more
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Chloroplast Transformation and Reverse Genetics

2006
For chloroplast transformation, the most efficient method to introduce DNA is particle bombardment. To then select the cells which harbor a transformed plastid, two classes of markers are available. With one class, selection is based on the rescue of a non-photosynthetic mutant with the wild-type chloroplast gene.
openaire   +2 more sources

Reverse Genetics of Influenza Virus

2014
Reverse genetics is the creation of a virus from a full-length cDNA copy of the viral genome, referred to as an "infectious clone," and is one of the most powerful genetic tools in modern virology. Since its development in 1999, plasmid-based reverse genetics has been effectively applied to numerous aspects of influenza studies which include ...
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Forward Genetics/Reverse Genetics

2010
Joris C Verster   +70 more
openaire   +1 more source

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