Results 11 to 20 of about 973,723 (161)

Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription

Viruses, 2009
There is ample evidence that synthesis of HIV-1 proviral DNA from the viral RNA genome during reverse transcription requires host factors. However, only a few cellular proteins have been described in detail that affect reverse transcription and interact ...
David Harrich, Luke Meredith, Kylie Warren, David Warrilow   +3 more
doaj   +1 more source

Blocking premature reverse transcription fails to rescue the HIV-1 nucleocapsid-mutant replication defect

Retrovirology, 2011
Background The nucleocapsid (NC) protein of HIV-1 is critical for viral replication. Mutational analyses have demonstrated its involvement in viral assembly, genome packaging, budding, maturation, reverse transcription, and integration.
Gorelick Robert J, Shatzer Teresa L, Thomas James A   +2 more
doaj   +1 more source

Strand transfer and elongation of HIV-1 reverse transcription is facilitated by cell factors in vitro.

PLoS ONE, 2010
Recent work suggests a role for multiple host factors in facilitating HIV-1 reverse transcription. Previously, we identified a cellular activity which increases the efficiency of HIV-1 reverse transcription in vitro.
David Warrilow, Kylie Warren, David Harrich   +2 more
doaj   +1 more source

Assessment of SARS-CoV-2 infection by Reverse transcription-PCR and serology in the Paris area: a cross-sectional study

BMJ Paediatrics Open, 2020
Background Several studies indicated that children seem to be less frequently infected with SARS-CoV-2 and are potentially less contagious than adults. To examine the spread of SARS-CoV-2, we combined both Reverse transcription-PCR testing and serology ...
Emmanuelle Varon, Stéphane Béchet, Corinne Levy, Robert Cohen, Camille Jung, Naim Ouldali, Odile Launay, Aurelie Sellam, Christophe Batard, Fabienne Cahn-Sellem, Annie Elbez, Alain Wollner, Olivier Romain, François Corrard, Said Aberrane, Nathalie Soismier, Rita Creidy, Mounira Smati-Lafarge   +17 more
doaj   +1 more source

Detection of SARS-CoV-2 Using Reverse Transcription Helicase Dependent Amplification and Reverse Transcription Loop-Mediated Amplification Combined with Lateral Flow Assay

Biomedicines, 2022
Rapid and accurate detection and identification of pathogens in clinical samples is essential for all infection diseases. However, in the case of epidemics, it plays a key role not only in the implementation of effective therapy but also in limiting the ...
Aleksandra Anna Zasada, Ewa Mosiej, Marta Prygiel, Maciej Polak, Karol Wdowiak, Kamila Formińska, Robert Ziółkowski, Kamil Żukowski, Kasper Marchlewicz, Adam Nowiński, Julia Nowińska, Waldemar Rastawicki, Elżbieta Malinowska   +12 more
doaj   +1 more source

Thermal Effects on Reverse Transcription: Improvement of Accuracy and Processivity in cDNA Synthesis

BioTechniques, 2001
Reverse transcription, coupled with DNA amplification, has been widely used for molecular analysis of RNAs. Reverse transcriptases are retroviral DNA polymerases that can synthesize DNA from both RNA and DNA.
Christine M. Malboeuf, Seth J. Isaacs, Nancy H. Tran, Baek Kim   +3 more
doaj   +1 more source

Magnetic quantitative reverse transcription PCR: A high-throughput method for mRNA extraction and quantitative reverse transcription PCR

BioTechniques, 2007
Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable.
Ricarda Jost, Oliver Berkowitz, Josette Masle   +2 more
doaj   +1 more source

Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells

Retrovirology, 2007
We have shown previously that HIV actively and selectively packages the spliced HIV RNAs into progeny virions. In the present study, by using a RT-QPCR and QPCR strategies, we show that spliced viral RNAs are present in infectious particles and ...
Morichaud Zakia, Houzet Laurent, Mougel Marylène   +2 more
doaj   +1 more source

Host SAMHD1 protein restricts endogenous reverse transcription of HIV-1 in nondividing macrophages

Retrovirology, 2018
Background SAM domain and HD domain containing protein 1 (SAMHD1) is a host anti-HIV-1 restriction factor known to suppress viral reverse transcription in nondividing myeloid cells by its dNTP triphosphorylase activity that depletes cellular dNTPs ...
Bijan Mahboubi, Christina Gavegnano, Dong-Hyun Kim, Raymond F. Schinazi, Baek Kim   +4 more
doaj   +1 more source

Augmentation of reverse transcription by integrase through an interaction with host factor, SIP1/Gemin2 Is critical for HIV-1 infection. [PDF]

PLoS ONE, 2009
There has been accumulating evidence for the involvement of retroviral integrase (IN) in the reverse transcription of viral RNA. We previously identified a host factor, survival motor neuron-interacting protein 1 (SIP1/Gemin2) that binds to human ...
Hironori Nishitsuji, Takaya Hayashi, Takuya Takahashi, Masashi Miyano, Mari Kannagi, Takao Masuda   +5 more
doaj   +1 more source