Results 21 to 30 of about 973,723 (161)

Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions

Viruses, 2021
The human immunodeficiency virus type 1 (HIV-1) capsid and its disassembly, or capsid uncoating, has remained an active area of study over the past several decades. Our understanding of the HIV-1 capsid as solely a protective shell has since shifted with
Zachary Ingram, Douglas K. Fischer, Zandrea Ambrose   +2 more
doaj   +1 more source

Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription

BioTechniques, 2012
Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses.
Lin Feng, Susanna Lintula, Tho Huu Ho, Maria Anastasina, Annukka Paju, Caj Haglund, Ulf-Håkan Stenman, Kristina Hotakainen, Arto Orpana, Denis Kainov, Jakob Stenman   +10 more
doaj   +1 more source

The Role of Capsid in the Early Steps of HIV-1 Infection: New Insights into the Core of the Matter

Viruses, 2021
In recent years, major advances in research and experimental approaches have significantly increased our knowledge on the role of the HIV-1 capsid in the virus life cycle, from reverse transcription to integration and gene expression.
Nawal AlBurtamani, Alwin Paul, Ariberto Fassati   +2 more
doaj   +1 more source

Initiation of HIV Reverse Transcription: Is Enzyme Flipping Required?

Viruses, 2011
Liu and colleagues have recently studied dynamic changes in the orientation of HIV reverse transcriptase (RT) on its nucleic acid substrate during initiation of DNA synthesis.
Matthias Götte
doaj   +1 more source

Tutorial: Guidelines for Single-Cell RT-qPCR

Cells, 2021
Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha, Mikael Kubista, Lukas Valihrach   +2 more
doaj   +1 more source

Reverse transcription of tRNA

Nucleic Acids Research, 1978
The 3' terminus of tRNA was enzymatically elongated by an oligo(A) tail. A fragment of DNA polymerase I (E. coli) was used in the presence of manganese to phase and synthesize a cleavable primer at the oligo(A)-tRNA template. When the threedimensional structure of oligo(A)-tRNA is being unfolded under conditions where the primer is still hybridized at ...
B, Wittig, S, Wittig
openaire   +3 more sources

HIV-1 Reverse Transcriptase Inhibition by Major Compounds in a Kenyan Multi-Herbal Composition (CareVid™): In Vitro and In Silico Contrast

Pharmaceuticals, 2021
CareVid is a multi-herbal product used in southwest Kenya as an immune booster and health tonic and has been anecdotally described as improving the condition of HIV-positive patients.
Winnie Rotich, Nicholas J. Sadgrove, Eduard Mas-Claret, Guillermo F. Padilla-González, Anastasia Guantai, Moses K. Langat   +5 more
doaj   +1 more source

When is it time for reverse transcription to start and go?

Retrovirology, 2009
Upon cell infection by a retrovirus, the viral DNA polymerase, called reverse transcriptase (RT), copies the genomic RNA to generate the proviral DNA flanked by two long terminal repeats (LTR).
Darlix Jean-Luc, Houzet Laurent, Mougel Marylène   +2 more
doaj   +1 more source

HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells

Retrovirology, 2008
Reverse transcription of the genetic material of human immunodeficiency virus type 1 (HIV-1) is a critical step in the replication cycle of this virus. This process, catalyzed by reverse transcriptase (RT), is well characterized at the biochemical level.
Ennifar Eric, Bader Thomas, Maidou-Peindara Priscilla, Lemay Julie, Rain Jean-Christophe, Benarous Richard, Liu Lang   +6 more
doaj   +1 more source

Primer tRNAs for reverse transcription [PDF]

Journal of Virology, 1997
Both retroviruses and long terminal repeat (LTR) retrotransposons use cellular tRNAs as primers for reverse transcription during their replication cycles. In retroviruses, primer tRNA is selectively packaged into the virion, where it is placed onto the primer binding site (PBS) of the viral RNA genome and used to prime the reverse transcriptase (RT ...
J, Mak, L, Kleiman
openaire   +2 more sources