Results 61 to 70 of about 16,252 (205)

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion [PDF]

, 2017
Recombinant protein production coopts the host cell machinery to provide high protein yields of industrial enzymes or biotherapeutics. However, since protein translation is energetically expensive and tightly controlled, it is unclear if highly expressed
Andersen, Mikael Rørdam, Beuchert Kallehauge, Thomas, Kildegaard, Helene Faustrup, Kwang Ha, Tae, Lewis, Nathan E., Ley, Daniel, Li, Shangzhong, Min Lee, Gyun, Pedersen, Lasse Ebdrup   +8 more
core   +3 more sources

A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling [PDF]

, 2019
Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3.
Atkinson, Gemma C, Graf, Michael, Hauryliuk, Vasili, Johansson, Marcus J O, Kasari, Villu, Margus, Tõnu, Murina, Victoriia, Nissan, Tracy, Nováček, Jiří, Pochopien, Agnieszka A, Turnbull, Kathryn, Wilson, Daniel N, Zhou, Yang   +12 more
core   +1 more source

Unraveling the Molecular Mechanisms of Glioma Recurrence: A Study Integrating Single‐Cell and Spatial Transcriptomics

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Glioma recurrence severely impacts patient prognosis, with current treatments showing limited efficacy. Traditional methods struggle to analyze recurrence mechanisms due to challenges in assessing tumor heterogeneity, spatial dynamics, and gene networks.
Lei Qiu, Yinjiao Fei, Jiaxuan Ding, Kexin Shi, Jinyan Luo, Yuchen Zhu, Xingjian Sun, Gefei Jiang, Yuandong Cao, Weilin Xu, Shu Zhou   +10 more
wiley   +1 more source

Atrophic Skeletal Muscle‐Derived Extracellular Vesicles Transfer miR‐125a‐5p to Inhibit Bone Formation in Osteoporosis during Aging

Advanced Science, EarlyView.
A muscle‐bone endocrine pathway in aging is revealed in which extracellular vesicles released from atrophic skeletal muscle (Aged‐SKM‐EVs) inhibit bone formation. These EVs deliver miR‐125a‐5p to osteoblasts, thereby suppressing the SIRT7‐Sp7 signaling axis and osteogenic differentiation.
Xiaoyan Shao, Pan Zhang, Zhidan Fan, Jiaquan Lin, Xiang Chen, Na Liu, Wang Gong, Yi He, Yining Zhou, Tianshu Shi, Yong Shi, Yuze Ma, Wentian Gao, Haosheng Wang, Depeng Fang, Chengzhi Wang, Wenshu Wu, Wenjin Yan, Jianghui Qin, Dongyang Chen, Haiguo Yu, Qing Jiang, Baosheng Guo   +22 more
wiley   +1 more source

Rocaglates induce gain-of-function alterations to eIF4A and eIF4F [PDF]

, 2020
Rocaglates are a diverse family of biologically active molecules that have gained tremendous interest in recent years due to their promising activities in pre-clinical cancer studies.
Baranov, Pavel V., Brown, Lauren E., Cencic, Regina, Chu, Jennifer, Devine, William G., Henkel, Thomas, Merrick, William C., O'Connor, Patrick B. F., Pelletier, Jerry, Porco, Jr., John A., Robert, Francis, Selznick, Asher, Zhang, Wenhan   +12 more
core   +1 more source

Circular RNA circNrip1 Interacts with SYNCRIP to Promote Neuropathic Pain by Stabilizing Tlr2 mRNA in Primary Sensory Neurons

Advanced Science, EarlyView.
Model of circNrip1 (cNrip1) upregulation driving neuropathic pain mechanisms. After peripheral nerve injury, increased FUS triggers the formation and upregulation of cNrip1 in injured DRG neurons. Upregulated cNrip1 recruits SYNCRIP to the 3′‐UTR of Tlr2 mRNA by binding to both, thereby promoting SYNCRIP‐triggered Tlr2 mRNA stability and increasing ...
Xiaozhou Feng, Jifang Xiao, Congcong Huang, Bao‐Chun Jiang, Bing Wang, Xu Li, Shibin Du, Tolga Berkman, Chun‐Hsien Wen, Weihua Cai, Dilip Sharma, Kun Wang, Longfei Ma, Huijuan Hu, Yuan‐Xiang Tao   +14 more
wiley   +1 more source

The ribosome profiling landscape of yeast reveals a high diversity in pervasive translation

Genome Biology
Background Pervasive translation is a widespread phenomenon that plays a critical role in the emergence of novel microproteins, but the diversity of translation patterns contributing to their generation remains unclear.
Chris Papadopoulos, Hugo Arbes, David Cornu, Nicolas Chevrollier, Sandra Blanchet, Paul Roginski, Camille Rabier, Safiya Atia, Olivier Lespinet, Olivier Namy, Anne Lopes   +10 more
doaj   +1 more source

Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii

Microbial Cell Factories, 2021
Background Eukaryotes use distinct networks of biogenesis factors to synthesize, fold, monitor, traffic, and secrete proteins. During heterologous expression, saturation of any of these networks may bottleneck titer and yield.
Troy R. Alva, Melanie Riera, Justin W. Chartron   +2 more
doaj   +1 more source

Translation inhibitors cause abnormalities in ribosome profiling experiments [PDF]

, 2014
Ribosome profiling and high-throughput sequencing provide unprecedented opportunities for the analysis of mRNA translation. Using this novel method, several studies have demonstrated the widespread role of short upstream reading frames in translational ...
Gerashchenko, Maxim V., Gladyshev, Vadim N.   +1 more
core   +1 more source

N4‐acetylcytidine in LncRNA Gm26917 Promotes Translation in Female Germline Stem Cells by Recruiting Ribosomal Protein mRNA via EEF1A1

Advanced Science, EarlyView.
This work establishes that ac4C modification on lncRNA Gm26917 governs its spatial interactions with Rpl10 mRNA, and RBP EEF1A1 mediates the interaction between Gm26917 and Rpl10. It elucidates a novel ac4C‐Gm26917‐EEF1A1‐Rpl10 axis in FGSC maintenance both in vitro and in vivo, and provides a potential molecular target for modulating germ cell ...
Xinyue Li, Xiaopeng Hu, Ji Wu
wiley   +1 more source