Results 81 to 90 of about 185,351 (165)

RiboSeq.Org:resources for the alignment, analysis and exploration of ribo-seq data

open access: yes, 2016
The ribosome profiling (ribo-seq) technique uses high-throughput sequencing to reveal the global locations and densities of ribosome protected mRNA fragments. On RiboSeq.Org (http://riboseq.org/) we provide freely available resources to help researchers analyse and explore ribo-seq data without having to use command-line tools.
Michel, Audrey   +9 more
openaire   +1 more source

nRibo-Seq

open access: yes, 2021
Measuring the time required for polysome assembly by Ribo-Seq of nascent ...
Johanna Schott
core   +2 more sources

Novel rRNA-depletion methods for total RNA sequencing and ribosome profiling developed for avian species

open access: yesPoultry Science, 2021
: Deep sequencing of RNAs has greatly aided the study of the transcriptome, enabling comprehensive gene expression profiling and the identification of novel transcripts. While messenger RNAs (mRNAs) are of the greatest interest in gene expression studies
Hanwen Gu, Yu H. Sun, Xin Zhiguo Li
doaj   +1 more source

Spatial Gene Expression in Fungi: mRNA Localization, Condensates, and Beyond

open access: yesYeast, EarlyView.
ABSTRACT Polarized growth and cellular asymmetry are broadly conserved properties of fungal life, from transient bud‐site selection in unicellular yeasts to sustained hyphal extension in filamentous and coenocytic species. While vesicle trafficking, cytoskeletal organization, and cell‐wall remodeling are well‐established contributors to these processes,
Sander van Otterdijk   +3 more
wiley   +1 more source

Derivation of Mouse Dome‐Shaped Trophoblast Stem Cells With Low Heterogeneity From Blastocysts

open access: yesCell Proliferation, EarlyView.
Here, Liu et al. report the generation of mouse dome‐shaped trophoblast stem cells (dTSCs) with low heterogeneity and autonomous differentiation potential. dTSCs robustly generate all trophoblast lineages in vitro and contribute to the placenta in vivo, providing a refined model for studying trophoblast development and related disorders.
Minli Liu   +8 more
wiley   +1 more source

Quality control analysis of Ribo-seq data.

open access: yes, 2023
(A) PCA of gene expression in Ribo-seq biological replicates, n = 3; see S1 Data. (B) Length distribution of RPFs; see S1 Data. All downstream analysis was restricted to the dominant fragment size of 26–29 nt.
Andrew A. Smith (17041553)   +9 more
core   +1 more source

Abridged Ribosome Profiling for Accurate Bacterial Translation Measurements

open access: yesMethods and Protocols
Ribosome profiling, or Ribo-Seq, is a powerful tool for studying translation. It maps the positions of translating ribosomes on mRNAs, providing insights into actively expressed genes. Unlike mass spectrometry, Ribo-Seq is not affected by the same biases
Marc Follmer   +2 more
doaj   +1 more source

Physiological media and oxygen levels modulate cancer hallmarks in cultured breast cancer cells

open access: yesThe FEBS Journal, EarlyView.
MCF7 breast cancer cells were cultured in standard conditions (18% O2 and DMEM) and physiological conditions (5% O2 and Plasmax, a medium modeled after the human plasma metabolome). A combined transcriptomic and proteomic approach was used to identify gene expression signatures at the mRNA and protein levels upstream of functional enrichment analyses ...
Jacob E. Wiebe   +5 more
wiley   +1 more source

gmtong/Ribo-seq-Tool-Comparison-Scripts: Ribo-seq-Tool-Comparison-Scripts

open access: yes
<p>Scripts for recreating analysis for the paper "Comparison of software packages for detecting unannotated small open reading frame translation by Ribo-seq"</p ...
gmtong
core   +1 more source

Point-Wise Ribosome Translation Speed Prediction with Recurrent Neural Networks

open access: yesMathematics
Escherichia coli is a benchmark organism, which has been deeply studied by the scientific community for decades, obtaining a vast amount of metabolic and genetic data.
Pietro Bongini   +6 more
doaj   +1 more source

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