Results 1 to 10 of about 98,629 (294)
Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni
eLife, 2019 CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma mansoni as proof of principle.
W. Ittiprasert +20 more
semanticscholar +3 more sources
ACS Central Science, 2020 COVID-19 is a global pandemic, thus requiring multiple strategies to develop modalities against it. Herein, we designed multiple bioactive small molecules that target a functional structure within the SARS-CoV-2’s RNA genome, the causative agent of COVID-
Hafeez S Haniff +10 more
semanticscholar +1 more source
Structural insight into precursor ribosomal RNA processing by ribonuclease MRP
Science, 2020 Adapted to the task at hand RNA-based catalysts perform fundamental tasks in cellular RNA metabolism, especially in eukaryotes, where RNAs are cut by specialized ribonucleoproteins (RNPs) as part of ribosome assembly or messenger RNA regulation or ...
P. Lan +6 more
semanticscholar +1 more source
Horse Pancreatic Ribonuclease [PDF]
European Journal of Biochemistry, 1974 Horse ribonuclease was purified after acid extraction of pancreas by ammonium sulfate fractionation and chromatography on CM‐cellulose. It is a glycoprotein with an average molecular weight of about 18000. The pure enzyme was chromatographically and electrophoretically heterogeneous due to the heterogeneity of the carbohydrate moiety.The amino‐acid ...
SCHEFFER, AJ, BEINTEMA, JJ
openaire +3 more sources
Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d
bioRxiv, 2018 CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications.
Cheng Zhang +9 more
semanticscholar +1 more source
A Type III CRISPR Ancillary Ribonuclease Degrades Its Cyclic Oligoadenylate Activator
bioRxiv, 2019 Cyclic oligoadenylate (cOA) secondary messengers are generated by type III CRISPR systems in response to viral infection. cOA allosterically activates the CRISPR ancillary ribonucleases Csx1/Csm6, which degrade RNA non-specifically using a HEPN (Higher ...
Januka S. Athukoralage +4 more
semanticscholar +1 more source
Philosophical Transactions of the Royal Society B: Biological Sciences, 2011 The gene coding for the RNA subunit of ribonuclease P (RNase P) is essential in all free-living organisms. The RNA subunit, itself, is an enzyme and, from its evolutionary tree, we can infer that it is a very ancient molecule. The specificity of this enzyme is that it cleaves other RNA molecules at the junction of single-stranded and the 5′ end of ...
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Ribonuclease H2 mutations induce a cGAS/STING‐dependent innate immune response
EMBO Journal, 2016 Aicardi–Goutières syndrome (AGS) provides a monogenic model of nucleic acid‐mediated inflammation relevant to the pathogenesis of systemic autoimmunity.
K. Mackenzie +16 more
semanticscholar +1 more source
CRYSTALLINE RIBONUCLEASE [PDF]
Journal of General Physiology, 1940 1. A crystalline enzyme capable of digesting yeast nucleic acid has been isolated from fresh beef pancreas. 2. The enzyme called "ribonuclease" is a soluble protein of albumin type. Its molecular weight is about 15,000. Its isoelectric point is in the region of pH 8.0. 3.
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The Immunomodulatory and Antimicrobial Properties of the Vertebrate Ribonuclease A Superfamily
Vaccines, 2018 The Ribonuclease A Superfamily is composed of cationic peptides that are secreted by immune cells and epithelial tissues. Although their physiological roles are unclear, several members of the vertebrate Ribonuclease A Superfamily demonstrate ...
L. Schwartz +3 more
semanticscholar +1 more source