Results 241 to 250 of about 2,736,514 (278)

Processing of RNA in Escherichia coli is limited in the absence of ribonuclease III, ribonuclease E and ribonuclease P

Journal of Molecular Biology, 1981
A strain of Escherichia coli lacking RNAase III and containing thermolabile RNAase E and RNAase P was labeled with 32Pi at a non-permissive temperature. RNA molecules were separated by two-dimensional polyacrylamide gel electrophoresis. Most of the small RNA species were isolated and analyzed for the presence of 5′ nucleoside triphosphates.
G, Plautz, D, Apirion
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Relaxation Spectra of Ribonuclease. III. Further Investigation of the Interaction of Ribonuclease and Cytidine 3'-Phosphate

Journal of the American Chemical Society, 1965
Gordon G Hammes, Renata E Cathou
exaly   +2 more sources

Mutational analysis of a ribonuclease III processing signal

Biochemistry, 1993
A mutational approach was employed to identify sequence and structural elements in a ribonuclease III processing signal that are important for in vitro enzymatic cleavage reactivity and selectivity. The substrate analyzed was the bacteriophage T7 R1.1 processing signal, a 60 nucleotide irregular RNA hairpin exhibiting an upper and lower dsRNA stem ...
B, Chelladurai, H, Li, K, Zhang, A W, Nicholson   +3 more
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Structural basis for non-catalytic and catalytic activities of ribonuclease III

Acta Crystallographica Section D: Biological Crystallography, 2006
Ribonuclease III (RNase III) represents a highly conserved family of double-stranded (ds) RNA-specific endoribonucleases, exemplified by bacterial RNase III and eukaryotic Rnt1p, Drosha and Dicer. Bacterial RNase III, containing an endonuclease domain followed by a dsRNA-binding domain, is the most extensively studied member of the family.
Xinhua Ji, Ji X
exaly   +3 more sources

Intermediate states of ribonuclease III in complex with double-stranded RNA. [PDF]

Structure, 2005
Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures ...
J. Gan, J. Tropea, B. P. Austin, D. Court, D. Waugh, X. Ji   +5 more
semanticscholar   +3 more sources

Processing of bacteriophage T4 primary transcripts with ribonuclease III

Journal of Molecular Biology, 1982
Abstract The two promoters (P 1 and P 2 ) of the cluster of transfer RNA genes of bacteriophage T4 are situated at distances of 0.95 × 10 3 and 1.3 × 10 3 bases, respectively, from the first tRNA gene. Isolated in vitro primary transcripts initiated at these promoters were incubated with highly purified RNase III. The resulting cleavage products
T, Barkay, A, Goldfarb
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Inhibitiory effects of gold(III) ions on ribonuclease and deoxyribonuclease

Journal of Inorganic Biochemistry, 2007
Inhibitory effects of gold(III) ions (Au(III)) on ribonuclease A (RNase A) and deoxyribonuclease I (DNase I) were investigated at neutral pH. RNase A was completely inhibited by 3 molar equivalents of Au(III) ions. DNase I was inhibited by 10 molar equivalents of Au(III) ions.
Tatsuo, Maruyama, Saori, Sonokawa, Hironari, Matsushita, Masahiro, Goto   +3 more
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Consequences of losing ribonuclease III on theEscherichia coli cell

Molecular and General Genetics MGG, 1976
An isogenic pair of Escherichia coli strains, one carrying an rnc+ and the other an rnc- allele (a mutation which reduces the level of ribonuclease III), was compared. The rnc- strain fails to grow at very elevated temperatures (for E. coli) while the rnc+ strain does grow exponentially.
D, Apirion, J, Neil, N, Watson
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A study of the mechanism of action of E. coli ribonuclease III

Biochimie, 1971
Summary The action of ribonuclease III, extracted from E. coli by the technique of Robertson et al., has been studied on synthetic and natural polyribonucleotides. An exhaustive hydrolysis of poly (G + C [3H]) and poly (I + C) [3H]) by RNase III, followed by an analysis of the hydrolysate on a DEAE-cellulose-urea column, showed that 80 p.
H. Schweitz, J. Ebel
semanticscholar   +2 more sources

Escherichia coli ribonuclease III

1990
Publisher Summary This chapter considers methods for the assay of RNase III, preparation of the substrates required, and a purification scheme for the enzyme that has been tested on strains of E. coli carrying cloned copies of the gene for RNase III (the rnc gene), which has recently been identified and sequenced.
openaire   +2 more sources