Results 211 to 220 of about 139,231 (236)

Isofocusing of antigenic small nuclear ribonucleoproteins

Analytical Biochemistry, 1985
In a companion report (T.B. Okarma, W.S. Schrier, and R. Feinbaum, 1985, Anal. Biochem. 147, 27-37) the behavior of small nuclear ribonucleoproteins (snRNPs) in native isofocusing gels was characterized. This communication extends those findings and describes a gentle procedure for the preparative isolation of snRNPs in native form from cultured murine
Rhonda Feinbaum, Thomas B. Okarma, Wayne H. Schrier   +2 more
openaire   +5 more sources

The use of immunoblotting and immunoprecipitation of (U) small nuclear ribonucleoproteins in the analysis of sera of patients with mixed connective tissue disease and systemic lupus erythematosus. A cross-sectional, longitudinal study.

Arthritis & Rheumatism, 1986
Antibodies to ribonucleoproteins (RNP) and to the Sm antigen in sera from patients with mixed connective tissue disease (MCTD) and systemic lupus erythematosus were studied using the techniques of immunoblotting and immunoprecipitation of U small nuclear
Ingvar Pettersson, Grace Wang, Elaine I. Smith, Hans Wigzell, Eva Hedfors, John Horn, Gordon C. Sharp   +6 more
semanticscholar   +1 more source

Structure and assembly of the spliceosomal small nuclear ribonucleoprotein particles

Current Opinion in Structural Biology, 1999
The spliceosome is a macromolecular assembly that carries out the excision of introns from nuclear pre-mRNAs. It consists of four large RNA-protein complexes, called the U1, U2, U4/U6 and U5 small nuclear ribonucleoproteins (snRNPs), and many protein factors.
Kiyoshi Nagai, C. Kambach, Stefan Walket
openaire   +3 more sources

Autoantibodies to small nuclear ribonucleoproteins. A strong association between anti-SS-B(La), HLA-B8, and Sjögren's syndrome.

Australian and New Zealand Journal of Medicine, 1983
The heterogeneity within multisystem autoimmune disease was evaluated according to the presence of antinuclear antibodies to ribonucleoproteins and the HLA-A1, B8, DR3 phenotype.
S. Whittingham, I. Mackay, B. Tait
semanticscholar   +1 more source

A subset of small nuclear ribonucleoprotein particle antigens is a component of the nuclear matrix

Biochemical and Biophysical Research Communications, 1982
Abstract We have assessed whether antigenic proteins associated with small nuclear ribonucleoprotein particles (snRNP) are associated with the nuclear matrix. Immunofluorescence studies showed that a subset of these particles (those reactive with anti-Sm antisera) were associated with the nuclear matrix, while a different set of particles (those ...
Vogelstein, B, Hunt, B F
openaire   +3 more sources

Autoantigens contained in the U1 small nuclear ribonucleoprotein complex [PDF]

, 1994
The U1 snRNP particle contains the following antigenic components: the specific proteins U1-A, U1–70K and U1-C, of which the U1–70K and the Ul-A polypeptides are the major targets of human autoantibodies (reviewed in [1]) Ul snRNA [2, 3].
Walther J. van Venrooij, Jacqueline M. T. Klein Gunnewiek   +1 more
openaire   +1 more source

Magnesium cations are required for the association of U small nuclear ribonucleoproteins and SR proteins with pre-mRNA in 200 S large nuclear ribonucleoprotein particles.

Journal of Molecular Biology, 1995
In previous studies we have shown that specific nuclear pre-mRNAs and their splicing products, as well as the general population of nuclear poly(A)+ RNA, are found packaged in 200 S large nuclear ribonucleoprotein (lnRNP) particles that represent the ...
E. Miriami, M. Angenitzki, R. Sperling, J. Sperling   +3 more
semanticscholar   +1 more source

Isolation of small nuclear ribonucleoprotein polypeptides

1990
Publisher Summary This chapter describes the biochemical and immunochemical isolation of the small nuclear ribonucleoprotein (snRNP) particles involved in RNA processing. While the snRNPs have been readily amenable to isolation, the individual protein species have not.
openaire   +3 more sources

A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro

Cell, 1986
RNAs containing the polyadenylation sites for adenovirus L3 or E2a mRNA or for SV40 early or late mRNA are substrates for cleavage and poly(A) addition in an extract of HeLa cell nuclei. When polyadenylation reactions are probed with ribonuclease T1 and antibodies directed against either the Sm protein determinant or the trimethylguanosine cap ...
Joan A. Steitz, Carl Hashimoto
openaire   +3 more sources

The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing

Nature, 1987
A small set of distinctive short RNA molecules are found in the nuclei of all higher eukaryotic cells and yeast, in protein complexes known as 'small nuclear ribonucleoprotein particles', or snRNPs. Recent work has confirmed early suggestions that these particles form part of the machinery by which primary RNA transcripts are processed to their mature,
Tom Maniatis, Robin Reed
openaire   +3 more sources