Results 221 to 230 of about 139,231 (236)

A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.

European Journal of Biochemistry, 1987
A hybridoma secreting a monoclonal antibody (H-20) that recognizes the 2,2,7-trimethylguanosine(m3G)-containing cap structure of U snRNAs was derived from a mouse which was immunized with a m3G-containing human serum albumin conjugate.
Peter Bochnig, Rolf Reuter, Peter Bringmann, Reinhard Lührmann   +3 more
semanticscholar   +1 more source

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

Cell, 1987
Electrophoretic separation of ribonucleoprotein particles in a nondenaturing gel was used to analyze the splicing of mRNA precursors. Early in the reaction, a complex formed consisting of the U2 small nuclear ribonucleoprotein particle (snRNP) bound to sequences upstream of the 3' splice site. This complex is modeled as a precursor of a larger complex,
Phillip A. Sharp, Maria M. Konarska
openaire   +3 more sources

The small nuclear ribonucleoproteins, SmB and B', are products of a single gene

Gene, 1991
Nucleotide sequencing of HeLa genomic DNA amplified by the polymerase chain reaction revealed 696-bp and 841-bp introns within the gene encoding the proteins SmB and SmB', respectively. Since splice consensus sequences were observed flanking the 145-bp insertion detected in the cDNA encoding SmB, we conclude that SmB and SmB' are derived from ...
Chu Jia-Li, Keith B. Elkon
openaire   +3 more sources

Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs

1990
Publisher Summary The snRNAs exist in the cell as ribonucleoprotein (RNP) complexes. While the snRNAs U1, U2, and U5 are organized in separate RNP particles, the majority of the snRNAs U4 and U6 reside in a single RNP complex. Several proteins in the molecular weight range of 25K to 200K were recently identified as possible U5-specific proteins ...
Montserrat Bach, Reinhard Lührmann, Peter Bringmann   +2 more
openaire   +3 more sources

Saccharomyces cerevisiae telomerase is an Sm small nuclear ribonucleoprotein particle

Nature, 1999
Activation of the chromosome end-replicating enzyme telomerase can greatly extend the lifespan of normal human cells and is associated with most human cancers. In all eukaryotes examined, telomerase has an RNA subunit, a conserved reverse transcriptase subunit and additional proteins, but little is known about the assembly of these components.
Sandra L. Wolin, Arthur J. Zaug, Anita G. Seto, Suzanne G. Sobel, Thomas R. Cech   +4 more
openaire   +3 more sources

The status of small nuclear RNA in the ribonucleoprotein fibrils containing heterogeneous nuclear RNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
hnRNP are made of two classes of unit, monoparticles and heterogeneous complexes. The monoparticles are much more easily dissociated by salt than the heterogeneous complexes. We made use of this differential salt sensitivity to determine the localization of snRNA in hnRNP.
Monique Jacob, Hélène Gallinaro
openaire   +2 more sources

Structural analysis of U small nuclear ribonucleoproteins by in vitro assembly

1990
Publisher Summary U small nuclear ribonucleoproteins (snRNPs) are essential cofactors in the splicing process of lower as well as higher eukaryotes. The major U snRNPs (U1, U2, U4-U6) of higher eukaryotes and the yeast counterparts are required to remove introns from messenger RNA precursors.
Jörg Hamm, Iain W. Mattaj
openaire   +3 more sources

[17] A guide for probing native small nuclear RNA and ribonucleoprotein structures

1989
Publisher Summary This chapter describes two methods that can be used for probing snRNA and small nuclear ribonucleoprotein (snRNP) structures, with special emphasis on the difficulties that may be encountered because of their content in highly structured regions. Both the methods involve structure-specific modifications or cleavages along RNA.
Alain Krol, Philippe Carbon
openaire   +3 more sources

Purification of small nuclear ribonucleoprotein particles active in RNA processing

1990
Publisher Summary The snRNPs are localized in the nucleoplasm, except for U3 and possibly U8 snRNPs which reside in the nucleolus. Each particle consists of a uridine-rich RNA, which is associated with several proteins. Except for U3, the snRNPs are immunoprecipitated with antibodies from patients suffering from connective tissue diseases.
openaire   +3 more sources

Purification of U Small Nuclear Ribonucleoprotein Particles

2003
Reinhard Lührmann, Berthold Kastner
openaire   +2 more sources