Results 51 to 60 of about 26,288 (294)

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes

STAR Protocols
Summary: The use of CRISPR-Cas9 ribonucleoproteins has revolutionized manipulation of genomes. Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.
Fernando H. Biase, Gustavo Schettini
doaj   +1 more source

Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles

Nature Communications, 2017
Transport of neo-synthesized influenza A virus viral ribonucleoproteins (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the mechanism is unclear.
Isabel Fernández de Castro Martin, Guillaume Fournier, Martin Sachse, Javier Pizarro-Cerda, Cristina Risco, Nadia Naffakh   +5 more
doaj   +1 more source

RNA-Seq of the nucleolus reveals abundant SNORD44-derived small RNAs. [PDF]

PLoS ONE, 2014
Small non-coding RNAs represent RNA species that are not translated to proteins, but which have diverse and broad functional activities in physiological and pathophysiological states. The knowledge of these small RNAs is rapidly expanding in part through
Baoyan Bai, Srinivasan Yegnasubramanian, Sarah J Wheelan, Marikki Laiho   +3 more
doaj   +1 more source

Double-gene targeting with preassembled Cas9 ribonucleoprotein for safe genome editing in the edible mushroomPleurotus ostreatus [PDF]

, 2023
Tatpong Boontawon, Takehito Nakazawa, Yeon-Jae Choi, Hyeon‐Su Ro, Minji Oh, Moriyuki Kawauchi, Masahiro Sakamoto, Yoichi Honda   +7 more
openalex   +1 more source

Membrane Fusion‐Inspired Nanomaterials: Emerging Strategies for Infectious Disease and Cancer Diagnostics

Advanced Healthcare Materials, EarlyView.
Membrane fusion‐inspired nanomaterials offer transformative potential in diagnostics by mimicking natural fusion processes to achieve highly sensitive and specific detection of disease biomarkers. This review highlights recent advancements in nanomaterial functionalization strategies, signal amplification systems, and stimuli‐responsive fusion designs,
Sojeong Lee, Khulan Nyamzaya, Jueun Han, Yejin Song, Jihee Lee, Jung Hyun Jo, Seungmin Bang, Eun‐Kyung Lim, Seungjoo Haam, Eunjung Kim   +9 more
wiley   +1 more source

Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 Ribonucleoproteins

Frontiers in Immunology, 2020
Natural killer (NK) cells are an attractive cell-type for adoptive immunotherapy, but challenges in preparation of therapeutic primary NK cells restrict patient accessibility to NK cell immunotherapy. NK-92 is a well-characterized human NK cell line that
Rih-Sheng Huang, Hsin-An Shih, Hsin-An Shih, Min-Chi Lai, Min-Chi Lai, Yao-Jen Chang, Steven Lin, Steven Lin   +7 more
doaj   +1 more source

NIR is degraded by the anaphase-promoting complex proteasome pathway [PDF]

Archives of Biological Sciences, 2014
Novel INHAT Repressor (NIR) is a histone acetylation inhibitor that can directly bind histone complexes and the tumor suppressors p53 and p63.
Jeong Ho Myong, Kang Kook Min, Kim Yang-Hee, Han Jin Seung   +3 more
doaj   +1 more source

Enhancing Magnetic Hyperthermia at the Cell Membrane by Anchoring 92R‐Functionalized Magnetic Nanoparticles to Low‐Endocytic CCR9 Surface Receptors

Advanced Healthcare Materials, EarlyView.
We present a strategy to enhance magnetic hyperthermia therapy by modulating nanoparticle–cell interactions. Antibody‐functionalized magnetic nanoparticles targeting the low‐internalizing CCR9 receptor enable spatially controlled membrane anchoring, reducing aggregation and maximizing heat generation under alternating magnetic fields.
David Egea‐Benavente, Isabel Corraliza‐Gorjón, Thomas S. van Zanten, María del Puerto Morales, Leonor Kremer, Domingo F. Barber   +5 more
wiley   +1 more source

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes

Nature Communications, 2017
Stress granules (SG) comprise aggregates of cellular messenger ribonucleoproteins (mRNPs) but how they form is unclear. Here, the authors identify NEDD4, an E3 ubiquitin ligase, as regulating the RNA binding protein NANOS2 and turnover of mRNP components,
Zhi Zhou, Hiroshi Kawabe, Atsushi Suzuki, Kaori Shinmyozu, Yumiko Saga   +4 more
doaj   +1 more source