Results 51 to 60 of about 122,430 (279)

TRPM8 levels determine tumor vulnerability to channel agonists

Molecular Oncology, EarlyView.
TRPM8 is a Ca2+ permissive channel. Regardless of the amount of its transcript, high levels of TRPM8 protein mark different tumors, including prostate, breast, colorectal, and lung carcinomas. Targeting TRPM8 with channel agonists stimulates inward calcium currents followed by emptying of cytosolic Ca2+ stores in cancer cells.
Alessandro Alaimo, Francesco Giuseppe Carbone, Kristi Buzo, Nicole Annesi, Sacha Genovesi, Annalisa Lorenzato, Karen Widmann, Michela Libergoli, Elisa Marmocchi, Giovanni Bertalot, Alberto Brolese, Mauro Giulio Papotti, Luca Molinaro, Orazio Caffo, Mattia Barbareschi, Alberto Bardelli, Alessandro Romanel, Sabrina Arena, Andrea Lunardi   +18 more
wiley   +1 more source

Association between semen microbiome disorder and sperm DNA damage

Microbiology Spectrum
DNA fragmentation index (DFI), a new biomarker to diagnose male infertility, is closely associated with poor reproductive outcomes. Previous research reported that seminal microbiome correlated with sperm DNA integrity, suggesting that the microbiome may
Junxian He, Menghui Ma, Zhenhan Xu, Jintao Guo, Haicheng Chen, Xing Yang, Peigen Chen, Guihua Liu   +7 more
doaj   +1 more source

Influence of 16S Ribosomal RNA Gene Polymerase Chain Reaction and Sequencing on Antibiotic Management of Bone and Joint Infections

Canadian Journal of Infectious Diseases and Medical Microbiology, 2013
INTRODUCTION: Amplification of the 16S ribosomal RNA gene by polymerase chain reaction (PCR) followed by analysis of generated sequences can be an important adjunct to conventional cultures.
B Alraddadi, S Al-Azri, KR Forward
doaj   +1 more source

Tick-associated diseases identified from hunting dogs during the COVID-19 pandemic in a Mayan community in Yucatan, Mexico

Open Veterinary Journal, 2023
Background: Hunting activity in the Mayan communities has increased due to COVID-19 and domestic dogs have gained more importance. Due to its proximity to humans, domestic dogs are a bridge between tick-borne diseases and humans and their peri-domestic ...
Karla Rossanet Dzul-Rosado, Karla Alejandra Arroyo-Solis, Adan Javier Torres-Monroy, Juan Jose Arias-Leon, Gaspar Fernando Peniche-Lara, Fernando Isaias Puerto-Manzano, Magaly Guadalupe Landa-Flores, Juan Carlos del Mazo-Lopez, Beatriz Salceda-Sanchez   +8 more
doaj   +1 more source

Use of RNA secondary structure for evolutionary relationships : investigating RNase P and RNase MRP : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University, New Zealand [PDF]

, 1998
Bioinformatics is applied here to examine whether RNA secondary structure data can reflect distant evolutionary relationships. This is important when there is little confidence in sequence data such as when looking at the evolution of RNase MRP (MRP ...
Collins, Lesley Joan
core  

Optimization of an in vitro transcription/translation system based on Sulfolobus solfataricus cell lysate. [PDF]

, 2018
A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for ...
Anna La Teana,, Benelli, Dario, Giuseppe, Perugino, LO GULLO, Giada, Londei, Paola, Rosanna, Mattossovich   +5 more
core   +2 more sources

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons [PDF]

Journal of Visualized Experiments, 2014
One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA.
Sanschagrin, Sylvie, Yergeau, Étienne
openaire   +3 more sources

The subcellular distribution of phosphorylated Y‐box‐binding protein‐1 at S102 in colorectal cancer patients, stratified by KRAS mutational status and clinicopathological features

Molecular Oncology, EarlyView.
This study identifies nuclear YB‐1 S102 phosphorylation as a marker associated with KRAS and FBXW7 mutations in colorectal cancer. Mutated KRAS correlates specifically with nuclear, not cytoplasmic, S102 YB‐1. These findings provide the first ex vivo evidence of this link in CRC and suggest future studies should assess the prognostic and therapeutic ...
Konstanze Lettau, Stephan Forchhammer, Birgit Fehrenbacher, Lejla Mahmutovic, Marcus Scharpf, Gunnar Blumenstock, Martin Schaller, Irina Bonzheim, Shayan Khozooei, Mahmoud Toulany   +9 more
wiley   +1 more source

The complete mitochondrial genome of Glossaulax reiniana (Littorinimorpha: Naticidae)

Mitochondrial DNA. Part B. Resources, 2018
In the present study, the complete mitochondrial genome of Glossaulax reiniana was determined using the next-generation sequencing. The circular genome was found to be 15,254 bp in length and had an overall nucleotide composition of 30.6% A, 14.1% C, 15 ...
Peng-Yu Li, Yi Yang, Yong-Guo Li, Shao-E Sun   +3 more
doaj   +1 more source

16S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring

Microbiology Indonesia, 2010
Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies.
AGUSTINA LULUSTYANINGATI NURUL AMININ1, FIDA MADAYANTI, PINGKAN ADITIAWATI, . AKHMALOKA   +3 more
doaj   +1 more source