Results 1 to 10 of about 1,834,577 (295)

GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins. [PDF]

Nature, 2022
Argonaute proteins use nucleic acid guides to find and bind specific DNA or RNA target sequences. Argonaute proteins have diverse biological functions and many retain their ancestral endoribonuclease activity, cleaving the phosphodiester bond between ...
Arif A, Bailey S, Izumi N, Anzelon TA, Ozata DM, Andersson C, Gainetdinov I, MacRae IJ, Tomari Y, Zamore PD.   +9 more
europepmc   +2 more sources

RNA polymerase I (Pol I) lobe-binding subunit Rpa12.2 promotes RNA cleavage and proofreading. [PDF]

J Biol Chem, 2022
Elongating nuclear RNA polymerases (Pols) frequently pause, backtrack, and are then reactivated by endonucleolytic cleavage. Pol backtracking and RNA cleavage are also crucial for proofreading, which contributes to transcription fidelity.
Schwank K, Schmid C, Fremter T, Milkereit P, Griesenbeck J, Tschochner H.   +5 more
europepmc   +2 more sources

Identification of human mitochondrial RNA cleavage sites and candidate RNA processing factors [PDF]

BMC Biology, 2022
Background The human mitochondrial genome is transcribed as long strands of RNA containing multiple genes, which require post-transcriptional cleavage and processing to release functional gene products that play vital roles in cellular energy production.
Guillermo Carbajosa, Aminah T. Ali, Alan Hodgkinson   +2 more
doaj   +2 more sources

Feature selection for RNA cleavage efficiency at specific sites using the LASSO regression model in Arabidopsis thaliana [PDF]

BMC Bioinformatics, 2021
Background RNA degradation is important for the regulation of gene expression. Despite the identification of proteins and sequences related to deadenylation-dependent RNA degradation in plants, endonucleolytic cleavage-dependent RNA degradation has not ...
Daishin Ueno, Harunori Kawabe, Shotaro Yamasaki, Taku Demura, Ko Kato   +4 more
doaj   +2 more sources

SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs: viral mRNAs are resistant to nsp1-induced RNA cleavage. [PDF]

PLoS Pathogens, 2011
SARS coronavirus (SCoV) nonstructural protein (nsp) 1, a potent inhibitor of host gene expression, possesses a unique mode of action: it binds to 40S ribosomes to inactivate their translation functions and induces host mRNA degradation.
Cheng Huang, Kumari G Lokugamage, Janet M Rozovics, Krishna Narayanan, Bert L Semler, Shinji Makino   +5 more
doaj   +2 more sources

Abrogation of PRRSV infectivity by CRISPR-Cas13b-mediated viral RNA cleavage in mammalian cells. [PDF]

Sci Rep, 2020
CRISPR/Cas9 enables dsDNA viral genome engineering. However, the lack of RNA targeting activities limits the ability of CRISPR/Cas9 to combat RNA viruses.
Cui J, Techakriengkrai N, Nedumpun T, Suradhat S.   +3 more
europepmc   +2 more sources

Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells [PDF]

Communications Biology
CRISPR-Cas13 exclusively targets RNA. In prokaryotes, Cas13 cleaves both target and non-target RNA indiscriminately upon activation by a specific target RNA, but in eukaryotic cells collateral cleavage activity has been limited.
Jorik Frederik Bot, Zhihan Zhao, Mengyuan Li, Darnell Kammeron, Peng Shang, Niels Geijsen   +5 more
doaj   +2 more sources

RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. [PDF]

Cell, 2009
Compelling evidence indicates that the CRISPR-Cas system protects prokaryotes from viruses and other potential genome invaders. This adaptive prokaryotic immune system arises from the clustered regularly interspaced short palindromic repeats (CRISPRs) found in prokaryotic genomes, which harbor short invader-derived sequences, and the CRISPR-associated (
Caryn R. Hale, Peng Zhao, S. Olson, M. Duff, B. Graveley, L. Wells, R. Terns, M. Terns   +7 more
semanticscholar   +3 more sources

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. [PDF]

Cell, 2017
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM ...
Liang Liu, Xueyan Li, Jun Ma, Z. Li, L. You, Jiuyu Wang, Min Wang, Xinzheng Zhang, Yanli Wang   +8 more
semanticscholar   +3 more sources

Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications [PDF]

Molecular Therapy: Nucleic Acids
The CRISPR-Cas13 system exhibits potent RNA cleavage activity and has been widely utilized for RNA-targeting applications. However, its collateral cleavage of bystander RNAs limits in vivo therapeutic applications. In this study, we generated a series of
Wenxia Zhang, Haili Wang, Dongzi Liu, Xi Mao, Yi Zhang, Yimu Yang, Zehui Liu, Tong Pan, Yingle Liu, Qi Zhang   +9 more
doaj   +2 more sources