A detailed protocol for RNA cleavage assay in sympathetic neurons [PDF]
STAR Protocols, 2021 Summary: This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons.
Catia Andreassi, Antonella Riccio
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Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity. [PDF]
Mol Cell, 2019 Type ΙΙΙ CRISPR-Cas systems provide robust immunity against foreign RNA and DNA by sequence-specific RNase and target RNA-activated sequence-nonspecific DNase and RNase activities.
Jia N +5 more
europepmc +2 more sources
Global analysis of RNA cleavage by 5′-hydroxyl RNA sequencing [PDF]
Nucleic Acids Research, 2015 RNA cleavage by some endoribonucleases and self-cleaving ribozymes produces RNA fragments with 5′-hydroxyl (5′-OH) and 2′,3′-cyclic phosphate termini.
S. Peach, Kerri T. York, J. Hesselberth
semanticscholar +3 more sources
An Ex Vivo Protocol to Assess IRE1α-Dependent RNA Cleavage Using Total RNA Isolated from Mouse Tissues [PDF]
Bio-ProtocolRegulated IRE1-dependent decay (RIDD) is a critical cellular mechanism mediated by the endoplasmic reticulum (ER) stress sensor IRE1α, which cleaves a variety of RNA targets to regulate ER homeostasis.
Mohammad Rashid +3 more
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Site specific target binding controls RNA cleavage efficiency by the Kaposi's sarcoma-associated herpesvirus endonuclease SOX. [PDF]
Nucleic Acids Res, 2018 During lytic replication of Kaposi’s sarcoma-associated herpesvirus (KSHV), the gene expression landscape of a cell is remodeled to evade the immune response and create an environment favorable to viral replication.
Mendez AS +3 more
europepmc +2 more sources
Mechanisms for ribotoxin-induced ribosomal RNA cleavage. [PDF]
Toxicology and Applied Pharmacology, 2012 The Type B trichothecene deoxynivalenol (DON), a ribotoxic mycotoxin known to contaminate cereal-based foods, induces ribosomal RNA (rRNA) cleavage in the macrophage via p38-directed activation of caspases. Here we employed the RAW 264.7 murine macrophage model to test the hypothesis that this rRNA cleavage pathway is similarly induced by other ...
Kaiyu He, Hui-Ren Zhou, J. Pestka
semanticscholar +3 more sources
In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay [PDF]
Bio-Protocol, 2019 The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube.
G. Karagöz +3 more
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Endonucleolytic RNA cleavage by a eukaryotic exosome [PDF]
Nature, 2008 The exosome is a major eukaryotic nuclease located in both the nucleus and the cytoplasm that contributes to the processing, quality control and/or turnover of a large number of cellular RNAs. This large macromolecular assembly has been described as a 3'-->5' exonuclease and shown to contain a nine-subunit ring structure evolutionarily related to ...
Alice Lebreton +3 more
semanticscholar +4 more sources
Critical factors for precise and efficient RNA cleavage by RNase Y in Staphylococcus aureus. [PDF]
PLoS GeneticsCellular processes require precise and specific gene regulation, in which continuous mRNA degradation is a major element. The mRNA degradation mechanisms should be able to degrade a wide range of different RNA substrates with high efficiency, but should ...
Alexandre Le Scornet +14 more
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Downstream sequence-dependent RNA cleavage and pausing by RNA polymerase I. [PDF]
J Biol Chem, 2020 The sequence of the DNA template has long been thought to influence the rate of transcription by DNA-dependent RNA polymerases, but the influence of DNA sequence on transcription elongation properties of eukaryotic RNA polymerase I (Pol I) from Saccharomyces cerevisiae has not been defined.
Scull CE +3 more
europepmc +3 more sources