Results 21 to 30 of about 1,834,577 (295)
Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis. [PDF]
Mol Cell, 2018 Rousseau BA +3 more
europepmc +2 more sources
Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. [PDF]
Proc Natl Acad Sci U S A, 2017 Pillon MC +4 more
europepmc +2 more sources
A bacterial Argonaute with efficient DNA and RNA cleavage activity guided by small DNA and RNA
bioRxiv, 2021 Argonaute proteins are widespread in prokaryotes and eukaryotes. Most prokaryotic Argonaute proteins (pAgos) use 5’P-gDNA to target complementary DNA.
Longyu Wang +11 more
semanticscholar +1 more source
RNA modifications can affect RNase H1-mediated PS-ASO activity
Molecular Therapy: Nucleic Acids, 2022 Phosphorothioate modified antisense oligonucleotides (PS-ASOs) can reduce gene expression through hybridization to target RNAs and subsequent cleavage by RNase H1.
Katelyn A. Doxtader Lacy +3 more
doaj +1 more source
RNase P RNA‐mediated cleavage [PDF]
IUBMB Life, 2009 AbstractMetal(II)‐induced hydrolysis of RNA produce products with 5′‐hydroxyls and 2′;3′‐cyclic phosphates at the ends. Ribozymes are RNA molecules that act as catalysts. Some ribozymes that cleave RNA also generate 5′‐hydroxyls and 2′;3′‐cyclic phosphates whereas others produces 5′‐phosphates and 3′‐hydroxyls at the ends of the cleavage products ...
Leif A, Kirsebom, Stefan, Trobro
openaire +3 more sources
Креативная хирургия и онкология, 2020 Background. With numerous extraction protocols for total RNA and RNA fractions, like microRNA (miRNA) and long non-coding RNA (lncRNA), available for various cell and tissue types, obtaining a high quantitative and qualitative yield from some special ...
I. F. Gareev +3 more
doaj +1 more source
STAR Protocols, 2022 Summary: Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation
Yajie Gao +3 more
doaj +1 more source
Biomolecules, 2022 Prokaryotic Argonautes (pAgos) from mesophilic bacteria are attracting increasing attention for their genome editing potential. So far, it has been reported that KmAgo from Kurthia massiliensis can utilize DNA and RNA guide of any sequence to effectively
Xiaoman Jiang +3 more
doaj +1 more source
Transfer RNA Cleavages by Onconase Reveal Unusual Cleavage Sites [PDF]
Journal of Biological Chemistry, 2006 Onconase, a protein from amphibian eggs and a homologue of pancreatic ribonuclease (RNase) superfamily, is cytotoxic, exhibits antitumor and antiviral activity, and is in phase III clinical trials. It has been shown to predominantly target cellular tRNA on its entry into mammalian cells (Saxena, S. K., Sirdeshmukh, R., Ardelt, W., Mikulski, S.
Avvaru N, Suhasini, Ravi, Sirdeshmukh
openaire +2 more sources
RNA secondary structure and nucleotide composition of the conserved hallmark sequence of Leishmania SIDER2 retroposons are essential for endonucleolytic cleavage and mRNA degradation. [PDF]
PLoS ONE, 2017 We have reported previously that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily, largely located within 3'UTRs of Leishmania transcripts, promote rapid turnover of mRNAs through endonucleolytic cleavage within the highly conserved ...
Hiva Azizi +6 more
doaj +1 more source