The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
Cell Reports, 2018 Summary: The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that
Cian J. Lynch +16 more
doaj +1 more source
iScience, 2023 Summary: THOC5, a member of the THO complex, is essential for the 3′processing of some inducible genes, the export of a subset of mRNAs and stem cell survival.
Mareike Polenkowski +10 more
doaj +1 more source
Impact of template backbone heterogeneity on RNA polymerase II transcription. [PDF]
, 2014 Variations in the sugar component (ribose or deoxyribose) and the nature of the phosphodiester linkage (3'-5' or 2'-5' orientation) have been a challenge for genetic information transfer from the very beginning of evolution.
Chong, Jenny +5 more
core +1 more source
Nucleolar RNA polymerase II drives ribosome biogenesis
Nature, 2020 Proteins are manufactured by ribosomes—macromolecular complexes of protein and RNA molecules that are assembled within major nuclear compartments called nucleoli 1 , 2 .
K. Abraham +24 more
semanticscholar +1 more source
Cap-specific terminal N6-methylation of RNA by an RNA polymerase II–associated methyltransferase
Science, 2019 A cap-specific m6A writer N6,2′-O-dimethyladenosine (m6Am) is present at the transcription start nucleotide of capped mRNAs in vertebrates. Akichika et al.
Shinichiro Akichika +10 more
semanticscholar +1 more source
Modulation of RNA primer formation by Mn(II)-substituted T7 DNA primase
Scientific Reports, 2017 Lagging strand DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase. The N-terminal primase domain of the gene 4 protein of phage T7 comprises a zinc-binding domain that recognizes a specific DNA sequence and an RNA polymerase ...
Stefan Ilic +7 more
doaj +1 more source
SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. [PDF]
PLoS ONE, 2011 BACKGROUND: Scaffold attachment factor A (SAF-A) participates in the regulation of gene expression by organizing chromatin into transcriptionally active domains and by interacting directly with RNA polymerase II. METHODOLOGY: Here we use co-localization,
Dzeneta Vizlin-Hodzic +4 more
doaj +1 more source
Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control [PDF]
, 2019 Processing and maturation of precursor RNA species is coupled to RNA polymerase II transcription. Co-transcriptional RNA processing helps to ensure efficient and proper capping, splicing, and 3' end processing of different RNA species to help ensure ...
Hughes, Katlyn D. +3 more
core +1 more source
Actively transcribing RNA polymerase II concentrates on spliced leader genes in the nucleus of Trypanosoma cruzi [PDF]
, 2005 RNA polymerase II of trypanosomes, early diverging eukaryotes, transcribes long polycistronic messages, which are not capped but are processed by trans-splicing and polyadenylation to form mature mRNAs.
Dossin, Fernando de Macedo +1 more
core +3 more sources
Structure of transcribing RNA polymerase II-nucleosome complex
Nature Communications, 2018 Eukaryotic transcription requires passage of RNA polymerase II (Pol II) through chromatin, which is impaired by nucleosomes. Here the authors report the cryo-EM structure of transcribing Pol II engaged with a downstream nucleosome core particle at an ...
Lucas Farnung +2 more
doaj +1 more source