Results 51 to 60 of about 495,103 (267)

Nanostructured RNA for RNA Intereference

YAKUGAKU ZASSHI, 2013
RNA interference (RNAi) is a potent and highly specific gene-silencing phenomenon which is initiated or triggered by double-stranded RNAs (dsRNAs). Shortly after the development of RNAi, small interfering RNAs (siRNAs) that are 21 nucleotides in length with a 3' nucleotide overhang were shown to be very effective in mammalian cells.
openaire   +3 more sources

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

RAID: a comprehensive resource for human RNA-associated (RNA–RNA/RNA–protein) interaction [PDF]

RNA, 2014
Transcriptomic analyses have revealed an unexpected complexity in the eukaryote transcriptome, which includes not only protein-coding transcripts but also an expanding catalog of noncoding RNAs (ncRNAs). Diverse coding and noncoding RNAs (ncRNAs) perform functions through interaction with each other in various cellular processes.
Zhang, Xiaomeng, Wu, Deng, Chen, Liqun, Li, Xiang, Yang, Jinxurong, Fan, Dandan, Dong, Tingting, Liu, Mingyue, Tan, Puwen, Xu, Jintian, Yi, Ying, Wang, Yuting, Zou, Hua, Hu, Yongfei, Fan, Kaili, Kang, Juanjuan, Huang, Yan, Miao, Zhengqiang, Bi, Miaoman, Jin, Nana, Li, Kongning, Li, Xia, Xu, Jianzhen, Wang, Dong   +23 more
openaire   +2 more sources

Cyclooxygenase-2 negatively regulates osteogenic differentiation in murine bone marrow mesenchymal stem cells via the FOXO3a/p27kip1 pathway

Bone & Joint Research
Aims: Cyclooxygenase-2 (COX-2) is an enzyme that synthesizes prostaglandins from arachidonic acid. Previous reports have indicated that COX-2 is constitutively expressed in osteogenic cells instead of being expressed only after pathogenic induction, and
Shu-Chun Chuang, Ya-Shuan Chou, Yi-Shan Lin, Je-Ken Chang, Chung-Hwan Chen, Mei-Ling Ho   +5 more
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Diversity and Versatility in Small RNA-Mediated Regulation in Bacterial Pathogens

Frontiers in Microbiology, 2021
Bacterial gene expression is under the control of a large set of molecules acting at multiple levels. In addition to the transcription factors (TFs) already known to be involved in global regulation of gene expression, small regulatory RNAs (sRNAs) are ...
Brice Felden, Yoann Augagneur
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

New insights into neurodevelopmental disorders by whole genome sequencing of 100 families from Italy

npj Genomic Medicine
Neurodevelopmental disorders (NDDs) have a strong but largely unexplained genetic basis. Moreover, the genetic architecture of these complex disorders in under-represented communities is poorly studied.
Giovanni Spirito, Sara Trova, Gaia Treves, Khudayar Farmanli, Mariacristina Franzese Canonico, Agata Fant, Stefano Marangoni, Federica Furia, Debora Charrance, Nicola Locci, Sara Gottardo, Francesca Groppo, Vittoria Perseghin, Martina Toscano, Erika Bibbò, Serena Donetti Dontin, Cecilia Cargnelutti, Mélody Colliard, Alessandro Rosina, Anna Maria Beoni, Cristina Bérard, Alessandro Coppe, Paolo Serravalle, Fabio Landuzzi, Francesco Musacchia, Antonio Amoroso, Remo Sanges, Andrea Cavalli, Manuela Vecchi, Laure Obino, Stefano Gustincich   +30 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source