Results 111 to 120 of about 365,508 (294)
Action of A RNase and AS RNase on growth of cells in vitro.
Folia biologica, 1977 The effect of bovine pancreatic A RNase and bull seminal vesicle AS RNase on proliferation of HeLa cells and LEP cells in vitro was studied. The ribonucleases were used in doses of 0.1, 1.0, 10.0, and 100.0 microgram per ml of medium and 100 000 inoculated cells. Proliferation was evaluated by the growth curves. Both in single and long-term experiments.
J, Cinátl, J, Matousek, R, Stanĕk
openaire +1 more source
Structural aspects of covalent and non-covalent dimers of pancreaticribonucleases
, 2010 The ability to retain ribonucleolytic activity in the presence of the ribonuclease inhibitor (RI) is one of the determinant of ribonucleases (RNases) cytotoxicity.
SICA, FILOMENA, MERLINO, ANTONELLO
core
Advanced Science, EarlyView.Glaucoma, a major cause of blindness, involves retinal ganglion cell (RGC) degeneration. This study shows growth hormone‐releasing hormone receptor (GHRHR) deficiency preserves RGC survival and restores vision, unlike activation which only aids survival.
Yan Tong +24 more
wiley +1 more source
Advanced Science, EarlyView.ABSTRACT The demand for off‐the‐shelf biocompatible bone substitutes has driven the development of numerous independent in vitro technologies to generate products resembling physiological tissues. Due to technical challenges and overly simplified cultivation approaches/niches, the end‐products are often uniformly shaped and inferior to native bone ...
Franziska Braun +10 more
wiley +1 more source
Structural insights into protein-only RNase P complexed with tRNA
, 2013 RNase P is the essential activity removing 5'-leader sequences from transfer RNA precursors. RNase P was always associated with ribonucleoprotein complexes before the discovery of protein-only RNase P enzymes called PRORPs (PROteinaceous RNase P) in ...
Gobert, Anthony +7 more
core +1 more source
Advanced Science, EarlyView.This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang +13 more
wiley +1 more source
Advanced Science, EarlyView.The present study shows that Nrf2 directly binds to the Gm26550 promoter, thereby activating Gm26550 transcription and increasing its expression. Mechanistically, Gm26550 promotes IGF1 expression by functionally antagonizing miR‐26a‐5p‐mediated repression and sequestering the RBP KHSRP, thereby enhancing hippocampal neuronal synaptic plasticity and ...
Hongfang Wang +12 more
wiley +1 more source
Unexpected DNA loss mediated by the DNA binding activity of ribonuclease A.
PLoS ONE, 2014 Ribonuclease A (RNase A) is widely used in molecular biology research both for analytical assays and for nucleic acid preparation. The catalytic mechanism of RNase A is well understood and absolutely precludes activity on DNA; however anecdotal reports ...
Federico Donà, Jonathan Houseley
doaj +1 more source
, 2017 RNase II is the most active exoribonuclease in Escherichia coli cell extracts. Yet, its removal appears to have no deleterious effect on growing cells. Here, we show that RNase II is required for cell survival during prolonged stationary phase and upon ...
Ernesto Quesada +5 more
core +1 more source
Advanced Science, EarlyView.Molecular editing of bilobalide uncovers BB56, a PREP‐engaging chemotype with enhanced anti‐inflammatory activity in microglia. Integrated CETSA‐MS, SAR, and pathway analyses link BB56 to attenuation of p38 MAPK/NF‐κB and NLRP3‐associated inflammatory signaling, highlighting bilobalide editing as a strategy for developing neuroinflammation‐modulating ...
Chanin Sillapachaiyaporn +20 more
wiley +1 more source