Results 31 to 40 of about 437,487 (317)

The Unfolding Behavior of RNase H Under Force [PDF]

Biophysical Journal, 2010
We have used optical tweezers to revisit the energy landscape of E. coli RNase H under mechanical force. This protein's equilibrium energetics and folding pathway have been studied in bulk and in single-molecule mechanical denaturation experiments, which showed the presence of a collapsed folding intermediate that is on-pathway to the native state (1 ...
Susan Marqusee, Jesse Dill, Carlos Bustamante   +2 more
openaire   +2 more sources

Biochemical characterization of a multi-drug resistant HIV-1 subtype AG reverse transcriptase: antagonism of AZT discrimination and excision pathways and sensitivity to RNase H inhibitors [PDF]

, 2016
We analyzed a multi-drug resistant (MR) HIV-1 re- verse transcriptase (RT), subcloned from a patient- derived subtype CRF02 AG, harboring 45 amino acid exchanges, amongst them four thymidine analog mutations (TAMs) relevant for high-level AZT (azi-
Bodem, Jochen, Buchholz, Bernd, Corona, Angela, Di Santo, Roberto, Jordan, Mareike, Maccioni, Elias, Schneider, Anna, Spã¶ring, Imke, Tramontano, Enzo, Wã¶hrl, Birgitta M.   +9 more
core   +1 more source

The Basic Loop of the RNase H Domain of MLV RT Is Important Both for RNase H and for Polymerase Activity

Virology, 2001
Escherichia coli RNase H has a basic extension that is involved in binding nucleic acid substrates. This basic extension is present in the RNase H of Moloney murine leukemia virus reverse transcriptase (MLV RT), but has been deleted from the RNase H of HIV-1 RT. Previous work showed that removing the basic loop from MLV RT (the mutant is called DeltaC)
Stephen H. Hughes, Paul L. Boyer, Hong-Qiang Gao, Patrick K. Clark, Peter Frank   +4 more
openaire   +3 more sources

Inhibitory effect of 2,3,5,6-tetrafluoro-4-[4-(Aryl)-1H-1,2,3-triazol-1-yl]benzenesulfonamide derivatives on HIV reverse transcriptase associated rnase H activities [PDF]

, 2016
The HIV-1 ribonuclease H (RNase H) function of the reverse transcriptase (RT) enzyme catalyzes the selective hydrolysis of the RNA strand of the RNA:DNA heteroduplex replication intermediate, and represents a suitable target for drug development.
Carcelli, Mauro, Corona, Angela, Esposito, Francesca, Grandi, Nicole, Naesens, Lieve, Pala, Nicolino, Palomba, Michele, Rogolino, Dominga, Sanna, Vanna, Sechi, Mario, Tramontano, Enzo   +10 more
core   +2 more sources

Selective inhibition of RNase H by dextran.

Journal of Biological Chemistry, 1981
Ordinarily, ribonuclease H hydrolyzes poly(rA) . poly(dT) and phiX174DNA-RNA at equal rates. Here we show that in the presence of dextran, the degradation of poly(rA) . poly(dT) is inhibited, while that of phi 174DNA-RNA is not. A similar inhibition by sucrose is found to be due to trace contamination of dextran in the sucrose. Ribose, deoxyribose, and
M L Dirksen, R J Crouch
openaire   +3 more sources

Polypurine Tract Formation by Ty1 RNase H [PDF]

Journal of Biological Chemistry, 2001
To better understand the mechanism by which Ty1 RNase H creates the polypurine tract (PPT) primer, we have demonstrated the polymerase-dependent hydrolytic activity of Ty1 reverse transcriptase (RT) during minus-strand synthesis. Using RNase H and polymerase mutants of the recombinant Ty1 RT protein, we show that the two domains of Ty1 RT can act ...
Ozcan Uzun, F.X. Wilhelm, Emilie H. Mules, M.L. Wilhelm, Abram Gabriel   +4 more
openaire   +3 more sources

Structure of HIV-1 reverse transcriptase cleaving RNA in an RNA/DNA hybrid [PDF]

, 2018
HIV-1 reverse transcriptase (RT) contains both DNA polymerase and RNase H activities to convert the viral genomic RNA to dsDNA in infected host cells. Here we report the 2.65-angstrom resolution structure of HIV-1 RT engaging in cleaving RNA in an RNA ...
KIM, MIN SUNG, Li, Hongzhi, Tian, Lan, Wang, Jimin, Yang, Wei   +4 more
core   +1 more source

Scaffold hopping and optimisation of 3’,4’-dihydroxyphenyl- containing thienopyrimidinones: synthesis of quinazolinone derivatives as novel allosteric inhibitors of HIV-1 reverse transcriptase-associated ribonuclease H

Journal of Enzyme Inhibition and Medicinal Chemistry, 2020
Bioisosteric replacement and scaffold hopping are powerful strategies in drug design useful for rationally modifying a hit compound towards novel lead therapeutic agents.
Graziella Tocco, Francesca Esposito, Pierluigi Caboni, Antonio Laus, John A. Beutler, Jennifer A. Wilson, Angela Corona, Stuart F. J. Le Grice, Enzo Tramontano   +8 more
doaj   +1 more source

Crystal structure of xenotropic murine leukaemia virus-related virus (XMRV) ribonuclease H [PDF]

, 2014
RNase H (retroviral ribonuclease H) cleaves the phosphate backbone of the RNA template within an RNA/DNA hybrid to complete the synthesis of double-stranded viral DNA.
Chung, Bong Hyun, Chung, Sang J., Erikson, Raymond Leo, Jung, Suk-Kyeong, Kang, Sunghyun, Kim, Bo Yeon, Kim, Ju Hee, Kim, Seung Jun, Yu, Keum Ran   +8 more
core   +1 more source

Silencing Antibiotic Resistance with Antisense Oligonucleotides

Biomedicines, 2021
Antisense technologies consist of the utilization of oligonucleotides or oligonucleotide analogs to interfere with undesirable biological processes, commonly through inhibition of expression of selected genes.
Saumya Jani, Maria Soledad Ramirez, Marcelo E. Tolmasky   +2 more
doaj   +1 more source