Results 31 to 40 of about 37,763 (207)

Clinical Validation and Evaluation of a Colorimetric SARS-CoV-2 RT-LAMP Assay Against RT-PCR [PDF]

, 2023
Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymer- ase chain reaction (RT-PCR).
ALP, ALPASLAN, Andac, Ayse, Erdem, Murat, Karahan, Gizem, Palaz, Fahreddin, Tozluyurt, Abdullah, Özketen, Ahmet Çağlar, ÜNAL, Serhat   +7 more
core   +1 more source

Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification.

PLoS Neglected Tropical Diseases, 2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak. Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a ...
Rokusuke Yoshikawa, Haruka Abe, Yui Igasaki, Saeki Negishi, Hiroaki Goto, Jiro Yasuda   +5 more
doaj   +1 more source

Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples

Bio-Protocol, 2021
During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse ...
Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Simon Anders, Michael Knop, Viet Dao Thi   +5 more
doaj   +1 more source

Compact highly sensitive photothermal RT-LAMP chip for simultaneous multidisease detection. [PDF]

Sci Adv
Developing instant detection systems with disease diagnostic capabilities holds immense importance for remote or resource-limited areas. However, the task of creating these systems—which are simultaneously easy to operate, rapid in detection, and cost-effective—remains a challenge.
Guo W, Tao Y, Yang R, Mao K, Zhou H, Xu M, Sun T, Li X, Shi C, Ge Z, Xue R, Zhou H, Ren Y.   +12 more
europepmc   +3 more sources

Nanomolecular detection of human influenza virus type A using reverse transcription loop-mediated isothermal amplification assisted with rod-shaped gold nanoparticles [PDF]

, 2014
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) and rod-shaped gold nanoparticles (gold nanorods; GNRs) were employed for nanomolecular detection of human influenza virus type A RNA.
Gill, P., Niazi, A., Nikbakht, H., Tabarraei, A.   +3 more
core   +1 more source

Evaluation of two lyophilized molecular assays to rapidly detect foot-and-mouth disease virus directly from clinical samples in field settings [PDF]

, 2017
Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot‐and‐mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories.
Armson, B., Chepkwony, E., Cleaveland, S., Fowler, V.L., Howson, E.L.A., Kandusi, S., Kasanga, C.J., King, D.P., Lembo, T., Madi, M., Martin, P., Mioulet, V., Sallu, R., Siddle, A., Wood, J.   +14 more
core   +1 more source

Comparative Diagnostic Performance of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Kit for the Rapid Detection of SARS-CoV-2

Pathogens, 2021
Although the reverse transcription-polymerase chain reaction (RT-PCR) is considered a standard-of-care assay for the laboratory diagnosis of SARS-CoV-2, several limitations of this method have been described.
Alexander Domnich, Andrea Orsi, Donatella Panatto, Vanessa De Pace, Valentina Ricucci, Patrizia Caligiuri, Giulia Guarona, Valerio Chessa, Diego Ferone, Simona Boccotti, Bianca Bruzzone, Giancarlo Icardi   +11 more
doaj   +1 more source

Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone [PDF]

, 2015
Ebola virus (EBOV) can lead to severe hemorrhagic fever with a high risk of death in humans and other primates. To guide treatment and prevent spread of the viral infection, a rapid and sensitive detection method is required for clinical samples.
Boxing Li, Chao Liu, Derong Dong, Erna Li, Huan Li, Jing Yuan, Lifei Cui, Puyuan Li, Wei Liu, Weishi Lin, Xiangna Zhao, Xiao Wei, Xuan Hu, Xuesong Wang, Yanyan Ma   +14 more
core   +2 more sources

Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) [PDF]

, 2018
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.).
AO Eni, AP Njukeng, AT Keizerweerd, Chukwuemeka K. Nkere, D Thomson, G Silva, Gabriel I. Atiri, Gonçalo Silva, HM Amer, J Thouvenel, Joseph Onyeka, Joshua O. Oyekanmi, M Parida, Moritz Bömer, N Maroya, Norbert G. Maroya, P Chomczynski, P. Lava Kumar, R Asiedu, RA Mumford, S Fukuta, Susan E. Seal, T Notomi, X Nie, Y Mori   +24 more
core   +2 more sources

Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using Reverse Transcription Loop-Mediated Isothermal Amplification Method

Animals, 2022
Porcine epidemic diarrhea virus (PEDV) can cause severe infectious porcine epidemic diarrhea (PED) and infect different ages of pigs, resulting in sickness and death among suckling pigs.
Chunhua Li, Jieling Liang, Dan Yang, Qi Zhang, Denian Miao, Xizhong He, Yanan Du, Wanjing Zhang, Jianping Ni, Kai Zhao   +9 more
doaj   +1 more source