Results 31 to 40 of about 37,268 (281)

Measures for diagnosing and treating infections by a novel coronavirus responsible for a pneumonia outbreak originating in Wuhan, China. [PDF]

, 2020
On 10 January 2020, a new coronavirus causing a pneumonia outbreak in Wuhan City in central China was denoted as 2019-nCoV by the World Health Organization (WHO).
Du, Lanying, Jiang, Shibo, Ojcius, David M, Pan, Chungen, Yu, Fei   +4 more
core   +1 more source

Development of a Saliva-Optimized RT-LAMP Assay for SARS-CoV-2 [PDF]

Journal of Biomolecular Techniques : JBT, 2021
AbstractConventional reverse transcription quantitative polymerase chain reaction (RT-qPCR) technology has struggled to fulfill the unprecedented need for diagnostic testing created by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic.
Albert D, Yu, Kristina, Galatsis, Jian, Zheng, Jasmine Quynh, Le, Dingbang, Ma, Stanley, Perlman, Michael, Rosbash   +6 more
openaire   +3 more sources

Rapid and sensitive detection of novel avian-origin influenza A (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device. [PDF]

PLoS ONE, 2013
A severe disease in humans caused by a novel avian-origin influenza A (H7N9) virus emerged in China recently, which has caused at least 128 cases and 26 deaths.
Yiyue Ge, Bin Wu, Xian Qi, Kangchen Zhao, Xiling Guo, Yefei Zhu, Yuhua Qi, Zhiyang Shi, Minghao Zhou, Hua Wang, Lunbiao Cui   +10 more
doaj   +1 more source

Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples

Bio-Protocol, 2021
During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse ...
Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Simon Anders, Michael Knop, Viet Dao Thi   +5 more
doaj   +1 more source

Nanomolecular detection of human influenza virus type A using reverse transcription loop-mediated isothermal amplification assisted with rod-shaped gold nanoparticles [PDF]

, 2014
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) and rod-shaped gold nanoparticles (gold nanorods; GNRs) were employed for nanomolecular detection of human influenza virus type A RNA.
Gill, P., Niazi, A., Nikbakht, H., Tabarraei, A.   +3 more
core   +1 more source

Evaluation of two lyophilized molecular assays to rapidly detect foot-and-mouth disease virus directly from clinical samples in field settings [PDF]

, 2017
Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot‐and‐mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories.
Armson, B., Chepkwony, E., Cleaveland, S., Fowler, V.L., Howson, E.L.A., Kandusi, S., Kasanga, C.J., King, D.P., Lembo, T., Madi, M., Martin, P., Mioulet, V., Sallu, R., Siddle, A., Wood, J.   +14 more
core   +1 more source

Survey and Visual Detection of Zaire ebolavirus in Clinical Samples Targeting the Nucleoprotein Gene in Sierra Leone [PDF]

, 2015
Ebola virus (EBOV) can lead to severe hemorrhagic fever with a high risk of death in humans and other primates. To guide treatment and prevent spread of the viral infection, a rapid and sensitive detection method is required for clinical samples.
Boxing Li, Chao Liu, Derong Dong, Erna Li, Huan Li, Jing Yuan, Lifei Cui, Puyuan Li, Wei Liu, Weishi Lin, Xiangna Zhao, Xiao Wei, Xuan Hu, Xuesong Wang, Yanyan Ma   +14 more
core   +2 more sources

Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) [PDF]

, 2018
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.).
AO Eni, AP Njukeng, AT Keizerweerd, Chukwuemeka K. Nkere, D Thomson, G Silva, Gabriel I. Atiri, Gonçalo Silva, HM Amer, J Thouvenel, Joseph Onyeka, Joshua O. Oyekanmi, M Parida, Moritz Bömer, N Maroya, Norbert G. Maroya, P Chomczynski, P. Lava Kumar, R Asiedu, RA Mumford, S Fukuta, Susan E. Seal, T Notomi, X Nie, Y Mori   +24 more
core   +2 more sources

Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using Reverse Transcription Loop-Mediated Isothermal Amplification Method

Animals, 2022
Porcine epidemic diarrhea virus (PEDV) can cause severe infectious porcine epidemic diarrhea (PED) and infect different ages of pigs, resulting in sickness and death among suckling pigs.
Chunhua Li, Jieling Liang, Dan Yang, Qi Zhang, Denian Miao, Xizhong He, Yanan Du, Wanjing Zhang, Jianping Ni, Kai Zhao   +9 more
doaj   +1 more source

Digital Loop-Mediated Isothermal Amplification on a Commercial Membrane [PDF]

, 2019
In this work, we report digital loop-mediated isothermal amplification (LAMP) or reverse-transcription LAMP (RT-LAMP) on a commercial membrane, without the need for complex chip fabrication or use of specialized equipment.
Hoffmann, Michael R., Huang, Xiao, Lin, Xingyu, Urmann, Katharina, Xie, Xing   +4 more
core   +4 more sources